C 150 (E2) 06/2011
page 55/78
Examples of general rules to reduce the contamination risks
•
Reduce hand germ count (wash hands with antimicrobial soap, dry with paper tissues, and rub dry
hands with alcoholic solution).
•
Wear appropriate clothing (work coat, shoes, face mask)
•
Keep as few personnel as possible in the cell culture lab.
Examples of sterile working method
•
Work "clean-to-dirty", i.e., handle confirmed uncontaminated cells first, unknown or untested cells
next, and lastly, if necessary, cells suspected to be contaminated.
•
Perform daily microscopic observations of cultures and specific tests for the bacteria and fungi as part
of a controlled routine. Test cultures for sterility before starting work.
•
Keep working surfaces clean. Immediately wipe spilled liquids with alcohol solutions.
•
No mouth contact on pipettes.
•
Never work on top of open sterile containers.
14.4 Chamber design and equipment of the CO
2
incubator C 150
The design concepts behind the CO
2
incubator C 150 considerably reduce the risk of contamination.
Among them are:
Even surfaces for easy manual cleaning
•
The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from a
single piece of steel, polished (suitable for pharmaceutical work) and has no welds or inaccessible
corners. The hinges and the seal of the inner glass door are glued from the outside, which also aids
cleaning of the inner chamber.
Removable parts for cleaning and autoclaving
•
The shelves are easily removed without screws. It is possible to autoclave the shelves. But this is
generally not necessary because they can remain inside the incubator during sterilization.
Door gasket
•
The inner door gasket is removable and autoclaveable.
Gas fine filter
•
The incoming gas used in the operation passes through a fine filter (aseptic filter, filtration efficiency
99.99%, particle size 0.45
μ
m) with a high filtration efficiency, which can also filter the smallest
particles (chap. 16.3).
CO
2
measuring system in the inner chamber
•
You can remove the CO
2
sensor from the inner chamber by hand for disinfection (chap. 15.3).
Condensation prevention
•
Condensation in the inner chamber represents a particular risk of contamination. The humidifying
system with water pan with integrated condensation point developed by BINDER is an effective and
easy way to ensure high humidity (95 ± 2% r.H.) inside the incubator without any condensation
forming on the inner surfaces.
Hot air sterilization at 180 °C /
356 °F
•
The heating system of the C 150 permits hot-air auto-sterilization (chap. 15.4). Thus, a temperature of
180 °C /
356°F
is maintained for a 30-minute period on all internal surfaces during its cycle, resulting
in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines
regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.
Summary of Contents for APT.line C 150
Page 75: ...C 150 E2 06 2011 page 75 78 18 9 Dimensions C 150...
Page 79: ......
