Binder APT.line C 150 Operating Manual Download

Page: 16 / 79

C 150 (E2) 06/2011

page 16/78

2.3 Connection panel at the rear of the unit

(11)

(12)

(13)

(14)

(15)

Figure 8: Rear connection panel C 150

(11) Power cable

(12) Miniature fuse

(13) Connection socket for zero-voltage relay alarm contact

(14) Quick acting closure socket for CO

gas bottle

(15) Ethernet interface for computer communication (option)

2.4

Triangle instrument panel C 150

Figure 9: Triangle instrument panel with RP1 controller

(2) Main power switch with protruding plastic shield (protection against turning off unintentionally)

(2)

Summary of Contents for APT.line C 150

Page 1: ...0080 9140 0080 right 100 V C150 9040 0083 9140 0083 left 100 V BINDER GmbH Address Post office box 102 D 78502 Tuttlingen Tel 49 7462 2005 0 Fax 49 7462 2005 100 Internet http www binder world com E...

Page 2: ...laments und des Rates vom 15 Dezember 2004 zur Angleichung der Rechtsvorschriften der Mitglied staaten ber die elektromagnetische Vertr glichkeit und zur Aufhebung der Richtlinie 89 336 EWG Directive...

Page 3: ...commande et de laboratoire Exigences relatives la CEM Partie 1 Exigences g n rales CEI 61326 1 2005 AC1 2008 NF EN 61326 1 2006 mod EN 61326 2 2 2006 Elektrische Mess Steuer Regel und Laborger te EMV...

Page 4: ...f delivery 17 3 2 Guidelines for safe lifting and transportation 18 3 3 Storage 19 3 4 Location of installation and ambient conditions 19 4 INSTALLATION AND CONNECTIONS 20 4 1 Shelves 20 4 2 CO2 senso...

Page 5: ...12 1 2Measuring CO2 directly via chemical indicator tubes 51 12 1 3Measuring CO2 directly with an electronic infrared measuring device 52 12 2 Temperature reference measurement 52 13 OPTIONS 52 13 1 S...

Page 6: ...al Republic of Germany 67 17 4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 68 17 5 Disposal of the unit in non member states of the EC 69 18 TECHNICA...

Page 7: ...ites for hazard free use and safety during operation and maintenance In no event shall BINDER be held liable for any dam ages direct or incidental arising out of or related to the use of this manual T...

Page 8: ...duct and or its functions or to property in its proximity 1 2 2 Safety alert symbol Use of the safety alert symbol indicates a risk of injury Observe all measures that are marked with the safety alert...

Page 9: ...he hazard mandatory action Observe all other notes and information not necessarily emphasized in the same way in order to avoid disruptions that could result in direct or indirect injury or property d...

Page 10: ...50 UL door hinged right Figure 4 Position of labels on the CO2 incubator C 150 UL door hinged left Keep safety labels complete and legible Replace safety labels that are no longer legible Contact BIND...

Page 11: ...arking Electrical and electronic equipment manufactured placed on the market in the EC after 13 August 2005 and to be disposed of in a separate collection according to directive 2002 96 EC on waste el...

Page 12: ...er of death Do NOT operate the unit in potentially explosive areas KEEP explosive dust or air solvent mixtures AWAY from the unit The CO2 incubator C 150 does not dispose of any measures of explosion...

Page 13: ...nded use Series C 150 incubators are suitable for the cultivation of mammal cells under typical conditions of approx 37 C 98 6 F The incubator permits setting defined pH conditions by common NaHCO3 bu...

Page 14: ...ng system in combination with the permanent mixture of CO2 gas through a special proprietary gas mixing head developed by BINDER allows precise and con stant CO2 concentrations for long periods This c...

Page 15: ...hinged right 1 Triangle instrument panel with RP1 controller for temperature and CO2 and main power switch 2 3 Gas mixing head CO2 4 CO2 sensor 5 Pt 100 temperature sensor 6 Shelves 7 Lower housing co...

Page 16: ...e 13 Connection socket for zero voltage relay alarm contact 14 Quick acting closure socket for CO2 gas bottle 15 Ethernet interface for computer communication option 2 4 Triangle instrument panel C 15...

Page 17: ...facturer may have caused traces of the shelves on the inner surfaces This has no impact on the function and performance of the unit Please remove any transportation protection devices and adhesives in...

Page 18: ...riginal packaging only Secure the CO2 incubator with transport straps for transport Do NOT lift or transport the unit using the door handle the door or the lower housing Lift the unit at the four lowe...

Page 19: ...id contamination never place the unit directly on the floor CAUTION Danger of overheating Damage to the unit Do NOT set up units in non ventilated recesses Ensure sufficient ventilation for dispersal...

Page 20: ...al pressure CO2 lost during 12h at 5 vol without door opening approx 2 g i e 0 001 cubic meters 0 035 cubic feet under normal pressure 1013 mbar 14 7 psi An example of how to evaluate laboratory volum...

Page 21: ...sor Do NOT expose the CO2 sensor to hot air sterilization The CO2 sensor head was especially adjusted for the specific chamber To avoid confusion an adhesive label with a serial number is adhered to t...

Page 22: ...ives is excluded from the liability agreement If required you can add microbiologically inhibiting substances such as copper chips copper sulphate or ethylene diamine tetra vinegar acid EDTA in a conc...

Page 23: ...a hose connection to the incubator The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data chap 18 4 and refer to a connection press...

Page 24: ...hose nozzle off the quick acting closure socket 4 4 3 Gas bottle connection kit option The following parts for connecting one bottle with the input connectors of the incubator are included Pressure r...

Page 25: ...d refer to a connection pressure of 2 0 bar 29 psi Decreasing supply pressure will result in longer recovery times 4 5 Electrical connection The CO2 incubator C 150 has a fixed power supply connection...

Page 26: ...day and in a well ventilated location 6 Function overview of the RP1 controller The RP1 controller controls the following values inside the CO2 incubator Temperature in C Range 7 C 12 6 F above ambien...

Page 27: ...m 1 0 vol Delay time tolerance range alarm CO2 after opening the door 15 min Password to lock unlock the HAND operating functions 0 Password to access operating mode USER 1 6 2 Performance after turni...

Page 28: ...bration time is approx 2 hours CO2 After approx 5 minutes the CO2 concentration equilibrates automatically to the preset value If there is no accordance between the actual and set value shown in the d...

Page 29: ...function i e the C 150 will from now on regulate at the new value If you do not want to accept the modification of a value wait 30 seconds without pressing any button The controller reverts to Normal...

Page 30: ...en exceeded the safety controller triggers an alarm For technical reasons the offset value should be at least 2 C factory setting 0 to 999 Max 3 digit numeric password for locking unlocking the operat...

Page 31: ...l the value OP U operating mode USER appears in the upper display Press MODE The display to enter the password appears The operating mode USER is password protected by a number The password is preset...

Page 32: ...99 min Temperature alarm delay time Period until the main controller triggers a tolerance range alarm after closing the door This prevents alarms constantly being triggered during the unstable operati...

Page 33: ...t required to operate the C 150 Its value should always be zero Set point type for safety controller Limit Lit In operating modes USER and HAND you can define the limit value Al S Set point type for s...

Page 34: ...djustment serves to adjust the temperature sensor OFF or 10 0 vol to 110 0 vol CO2 final value adjustment serves to adjust the CO2 sensor The operating function At S specifies the safety controller se...

Page 35: ...n the upper display OP L operating mode LOCK is now displayed It is visible only if a password was set in operating mode HAND via operating function PA H Confirm the selected operating mode with the M...

Page 36: ...all controller functions are taken out of operation The gas inlet valves are closed so that no gas can escape into the ambient air The zero voltage relay alarm output 13 chap 10 3 is switched to alarm...

Page 37: ...R with the arrow keys Password request Password entry factory setting 1 4 x Entry of altitude Entry of the current altitude km above sea level with arrow keys After 30 seconds the controller reverts t...

Page 38: ...oint Entry of CO2 set point with arrow keys After 30 seconds the controller reverts to Normal Display automatically When setting a lower temperature set point in order to save time we recommend coolin...

Page 39: ...s safety controller activity The LED ALARM flashes the LED is lit At the same time there is an additional audible alert buzzer Pressing the EXIT button turns off the audible alarm When the temperature...

Page 40: ...ER Operating mode HAND Operating mode USER If operating mode HAND appears toggle to operating mode USER with the arrow keys Password request Password entry factory setting 1 12 x Selecting the set poi...

Page 41: ...AND and USER You can set a limit value With set point type Offset OFS operating function Ot S safety controller set point as offset value is displayed in operating modes HAND and USER You can set an o...

Page 42: ...p 9 2 1 a different display appears Entry of limit value or offset value With set point type Limit Enter a limit value Entry of limit value using the arrow keys or With set point type Offset Enter an...

Page 43: ...sensor for interior heating CO2 sensor not connected If more than one alarm signal is sent simultaneously they are displayed in a cycle except for alarms 995 to 999 These take priority over all other...

Page 44: ...nd connection socket Do NOT exceed the maximum switching load of 24V AC DC 2 0 Amp Do NOT connect any devices with a higher loading capacity The alarm message on the controller display remains display...

Page 45: ...ure sufficient ventilation around the installation location to prevent any buildup of heat in the unit Check whether the sterilization cycle was aborted and the unit has returned to its standard opera...

Page 46: ...contact BINDER Service To decrease the temperature proceed as follows Turn off the unit Open both unit doors for approx 5 minutes Turn on the unit again You can restart normal operation as soon as th...

Page 47: ...heck that the outer door is closed properly Check the door s seals for any damage Replace any damaged seals If the above points do not reveal the source of the fault it may be that the unit is faulty...

Page 48: ...sure reducer of each bottle The recovery times of the gas concentrations inside the chamber following the door being opened which are indicated in the technical data chap 18 4 refer to a connection pr...

Page 49: ...BINDER Service 11 Error messages While the controller is operating or when you turn it on or change the operation mode fault signals may be emitted that are caused by the controller malfunctioning int...

Page 50: ...d base equilibrium of the buffer system in the culture media NaHCO3 buffers the common media From the pH value of the medium it is possible to conclude its CO2 concentration Figure 27 shows the relati...

Page 51: ...of the inner chamber volume by pressing the pump fully together and releasing it afterwards 5 The standardized volume is suctioned through the glass tube and the chemical indicator changes its color b...

Page 52: ...vice it is important to use a device traceable to an acknowledged standards calibration institu tion DKD PTB for Germany with valid calibration certificate Note The cable of the sensor must be thin en...

Page 53: ...acking adapter for direct thermal de coupled stacking of two incubators The stacking adapter ensures the exact maintenance of the set incubation parameters also during sterili zation of the other unit...

Page 54: ...ing fea tures and the lab personnel Keep pipettes and instruments sterile after autoclaving Bio safety cabinets laminar air flow should have a minimum of items apart from aspirator tube and burner Ite...

Page 55: ...ass door are glued from the outside which also aids cleaning of the inner chamber Removable parts for cleaning and autoclaving The shelves are easily removed without screws It is possible to autoclave...

Page 56: ...light If the temperature distribution inside the chamber becomes uneven condensation may form on the cooler surfaces Maintain distances from the wall rear 100 mm 3 94 in sides 50 mm 1 97 in Do not pla...

Page 57: ...sing the neutral cleaning agent Art No 1002 0016 CO2 sensor Alcohol based solutions Do not immerse the CO2 sensor into the solution Disinfection with alcohol or an alcohol based surface disinfectant w...

Page 58: ...y dry and ventilated as explosive gases may form during the sterilization process 2 You can sterilize the shelves in a sterilizer or autoclave With every decontamination disinfection method always use...

Page 59: ...the CO2 sensor during operation Damage to the CO2 sensor Connect or remove the CO2 sensor only with the unit turned off Recommended procedure 1 Turn off the unit 2 Pull out the sensor 3 Spray the sens...

Page 60: ...e to the CO2 sensor Do NOT expose the CO2 sensor to hot air sterilization The CO2 sensor head was especially adjusted for your specific chamber To avoid confusion an adhe sive label with a serial numb...

Page 61: ...ode HAND is locked This is displayed by an error message If this happens unlock the operating mode HAND chap 7 4 Figure 33 Error message if operating mode HAND is lo cked or if the CO2 sensor is still...

Page 62: ...mum temperature of 60 C 140 F Turn on the unit chap 5 The unit is now ready to operate 15 5 Aborting the hot air sterilization If the sterilization procedure is aborted prematurely whether effective s...

Page 63: ...When the inner chamber has cooled down to a value below 60 C 140 F turn off the unit and plug in the CO2 sensor The CO2 sensor is temperature resistant up to a maximum temperature of 60 C 140 F Turn o...

Page 64: ...mperature alarm the C 150 will trigger alarm signals You can ignore this alarm and turn off the buzzer of the safety controller by pressing EXIT If necessary repeat the sterilization When the inner ch...

Page 65: ...hotline Asia Pacific 852 39070500 or 852 39070503 BINDER service hotline Russia and CIS 7 495 98815 17 BINDER Internet website http www binder world com BINDER address BINDER GmbH post office box 102...

Page 66: ...ete address contact person and availability of that person Exact location of the BINDER product in your facility A contamination clearance certificate chap 19 must be faxed in advance The authorizatio...

Page 67: ...indications for appropriate storage chap 3 3 Final decommissioning Dispose of the unit as described in chap 17 3 to 17 5 When restarting the unit please pay attention to the corresponding information...

Page 68: ...tion clearance certificate chap 19 and enclose it with the unit WARNING Contamination of the device with toxic infectious or radioactive substances Danger of intoxication Danger of infection NEVER tak...

Page 69: ...rom the unit Prior to disposal disinfect the unit from all sources of infection Be aware that sources of infection may also be located outside the inner chamber If you cannot safely remove all sources...

Page 70: ...y bearing a valid calibration certificate During factory calibration and adjustment test gases with an analyzed concentration and with adapted flow quantity serve to calibrate the sensor system for CO...

Page 71: ...erature data Temperature range 7 C 12 6 F above ambient up to C F 50 122 Temperature fluctuation K 0 1 Temperature uniformity variation at 37 C 98 6 F K 0 4 Recovery time 1 after door open for 30 sec...

Page 72: ...es 2 Use this value for sizing air condition systems All technical data is specified for units with standard equipment at an ambient temperature of 25 C 77 F and a power supply voltage fluctuation of...

Page 73: ...auto diagnosis system with zero voltage relay output Temperature safety device class 3 1 acc to DIN 12880 Lockable door Tightly closing inner glass door 3 perforated shelves stainless steel 1 4301 V2...

Page 74: ...03 Manual for primary human cell culture 7001 0079 Petri dish incubation rack rack for 6 x 6 Petri dishes stainless steel 6006 0167 Petri dish incubation rack rack for 6 Petri dishes stainless steel 6...

Page 75: ...C 150 E2 06 2011 page 75 78 18 9 Dimensions C 150...

Page 76: ...t Bauteil eintrifft Eine weitere Ko pie soll dem Ger t Bauteil beigef gt sein Ggf ist auch die Spedition zu informieren Incomplete information or non conformity with this procedure will inevitably lea...

Page 77: ...substances weder giftige noch sonstige gef hrliche Stoffe enth lt oder solche anhaften That eventually generated reaction products are non toxic and also do not represent a hazard auch evtl entstande...

Page 78: ...en entsteht haften und BINDER gegen eventuell entstehende Schadenanspr che Dritter freistellen We are aware that in accordance with Article 823 of the German Civil Code BGB we are directly liable with...

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