Beckman Coulter Microfuge 20 Series Instructions For Use Manual Download Page 1

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B30162AA
May 2013

Instructions For Use

Microfuge 20 Series

Centrifuges

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Summary of Contents for Microfuge 20 Series

Page 1: ...B30162AA May 2013 Instructions For Use Microfuge 20 Series Centrifuges Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A ...

Page 2: ...prior written permission from Beckman Coulter Inc Beckman Coulter and the stylized logo are trademarks of Beckman Coulter Inc and are registered in the USPTO All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Find us on the World Wide Web at www beckmancoulter com Beckman Coulter Ireland Inc Mervue Business Park Mervue Galway...

Page 3: ...B30162AA iii Revision Status Initial Issue 05 2013 Microfuge 20 Series Centrifuges Instructions for Use version B30162AA ...

Page 4: ...B30162AA iv Revision Status ...

Page 5: ...es IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety During Installation and or Maintenance The ce...

Page 6: ...y Safety Against Risk of Fire This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in this centrifuge nor handle or store them within the 30 cm 12 inches clearance envelope surrounding the centrifuge Do not use the centrifuge within hazardous locations Mechanical Safety For sa...

Page 7: ...st virulent Hepatitis Band C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive m...

Page 8: ...B30162AA viii Safety Notice Chemical and Biological Safety ...

Page 9: ...otor Chamber 1 4 Drive 1 4 Temperature Sensing and Control refrigerated models only 1 4 Controls and Indicators 1 5 Power Switch 1 5 Control Panel 1 5 System Keys 1 5 Program Keys 1 6 Digital Displays 1 6 Name Rating Plate 1 7 Microfuge 20 Nonrefrigerated Model Specifications 1 8 Microfuge 20R Refrigerated Model Specifications 1 9 Available Rotors 1 10 CHAPTER 2 Operation 2 1 Introduction 2 1 Run ...

Page 10: ...d Soft stop functions 2 10 Starting a Run 2 10 To start a run 2 10 Short Run Quick Run Function 2 11 To perform a quick run on the non refrigerated Microfuge 20 2 11 To perform a quick run on the refrigerated Microfuge 20R 2 12 Changing Parameters During a Run 2 12 To change the speed during a run 2 12 To change the run time during a run 2 13 To change the temperature during a run Microfuge 20R on...

Page 11: ...age and Transport 4 4 Storage 4 4 Notes on Transport 4 4 Returning a Centrifuge 4 5 Supply List 4 5 Replacement Parts 4 5 Other 4 5 APPENDIX A Installation A 1 Introduction A 1 Installing the Instrument A 1 To install the centrifuge A 2 Electrical Requirements A 3 Test Run A 5 APPENDIX B Table of Hazardous Substance s Name and Concentration B 1 Beckman Coulter Inc Microfuge 20 Series Centrifuge Wa...

Page 12: ...l Panels 1 5 1 5 Digital Display showing all possible displays Microfuge 20R shown 1 7 2 1 Tapered Sleeve Side View 2 3 2 2 Default Parameters on First Time Use Microfuge 20R shown 2 4 3 1 Error message example 3 2 3 2 Removing the Emergency Access Plug 3 7 3 3 Manually releasing the door lock with the Allen wrench 3 7 A 1 Dimensions of the Non Refrigerated Centrifuge A 3 A 2 Dimensions of the Ref...

Page 13: ...xiii Tables Tables 1 1 Display Definitions 1 7 2 1 Acceleration and Deceleration Rates by Rotor in minutes seconds 2 9 3 1 Error Message Chart 3 3 3 2 Troubleshooting Chart 3 5 ...

Page 14: ...xiv Tables ...

Page 15: ...centrifuge performance A list of available rotors is also included CHAPTER 1 Description provides a brief physical and functional description of the centrifuge the operating controls and indicators and system specifications CHAPTER 2 Operation contains centrifuge operating procedures CHAPTER 3 Troubleshooting lists diagnostic messages and other possible malfunctions together with probable causes a...

Page 16: ...bold type Cursorkeys used to increment values up or downwhen setting parameters are shownas up and down arrows or CFC Free Centrifugation To ensure minimal environmental impact no CFCs are used in the manufacture or operation of Microfuge 20 series centrifuges Symbols and Labels This section provides information for some labels and symbols appearing on the Microfuge 20 instrument housing These lab...

Page 17: ...hazardous parts could occur possibly resulting in electric shock should only be performed by your Beckman Coulter representative Protective Ground This symbol is used to indicate a protective ground This instrument must be properly grounded Do not under any circumstances operate the instrument unless it is properly grounded Recycling Label This symbol is required in accordance with the Waste Elect...

Page 18: ...take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device Risk of Fire Warning Before replacing fuses shut off power and disconnect the power cord Failure to do so can cause electric shock and or equipment damage Replace fuses only with approved type and rating replacement fuse Electric Shock Warning Operation replacement or servicing...

Page 19: ...indicates the environmental protection use period for the product The outer circle indicates that the product can be recycled The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired The date on the label indicates the date of manufacture Australian EMC Compliance Label The C Tick mark is intended for use on products that com...

Page 20: ...e should be referred to for more information RoHS Conform Label This label indicates that the product is compliant with the EU RoHS directive 2002 95 EC pertaining to the Restriction of Hazardous Substances in Electrical and Electronic Equipment EEE TUV NRTL Label This label indicates recognition by a Nationally Recognized Test Laboratory NRTL that the instrument has met the relevant product safet...

Page 21: ...e also described instructions for their use are in CHAPTER 2 Operation Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances publication IN 175 Refer to the applicable rotor manuals for rotor descriptions Centrifuge Function and Safety Features Centrifuge Function Figure 1 1 Microfuge 20 A and Microfuge 20R B Front View 1 Door 2 Display 3 Emergency Door R...

Page 22: ...phase separations and receptor binding Rapid sedimentation of protein precipitates particulates and cell debris The centrifuges are microprocessor controlled providing interactive operation The instrument design features a brushless three phase drive system and a choice of acceleration deceleration rates The refrigerated models also have temperature control systems Models Microfuge 20 series centr...

Page 23: ... sensor on the drive motor Throughout the run checks are made to ensure that the rotor does not exceed set speed The centrifuge feet made of rubber have been designed to minimize possible rotation in the event of a rotor mishap An internal system continually monitors the sensor signals to confirm that they are within the expected values Malfunctions are indicated by error messages witha number in ...

Page 24: ...toreducedecelerationtime allowing fast processing of samples alternately delicate gradients may be preserved using slower deceleration Temperature Sensing and Control refrigerated models only With the power on the temperature control system is activated when the door is closed The run temperature can be set between 10 and 40 C in refrigerated models If no set temperature is entered the centrifuge ...

Page 25: ...trols electrical power to the centrifuge NOTE The power must be turned on before the chamber door can be opened Control Panel The control panel is mounted at an angle on the front of the centrifuge and includes system keys programming keys and digital displays see Figure 1 4 Figure 1 4 Microfuge 20 and Microfuge 20R Control Panels System Keys The centrifuge operation is controlled through the syst...

Page 26: ...STOP key can be pressed to end a run The centrifuge decelerates to a complete stop according to the preselected deceleration curve Deceleration can be terminated and the centrifuge restarted by pressing START again OPEN Pressing the OPEN key unlatches the centrifuge door lock and allows the door to be opened The centrifuge accepts this command only when the rotor is completely stopped QUICK RUN Ma...

Page 27: ...own when corresponding with Beckman Coulter regarding your centrifuge Table 1 1 Display Definitions Display Description Soft Stop Start Off Acceleration and Deceleration settings Short Run Displays while the Quick Run button Microfuge 20 only is pressed or when the Start button is pressed and held Microfuge 20R only Prog Displays to show the saved program number Set Displayed when the speed run ti...

Page 28: ... times Deceleration Fast soft See Acceleration and Deceleration Rates by Rotor in minutes seconds for times Programs 10 Ambient temperature range 5 to 40 C Humidity restrictions 80 noncondensing Maximum altitude 2000 m 6562 ft Dimensions Width Depth Height door closed Height door open 31 cm 12 2 in 42 cm 16 5 in 27 cm 10 7 in 53 cm 20 7 in Weight 13 kg 28 6 lb Clearances sides 30 cm 11 8 in Electr...

Page 29: ...Acceleration and Deceleration Rates by Rotor in minutes seconds for times Deceleration Fast soft See Acceleration and Deceleration Rates by Rotor in minutes seconds for times Programs 10 Ambient temperature range 5 to 35 C Humidity restrictions 80 noncondensing Maximum altitude 2000 m 6562 ft Dimensions Width Depth Height door closed Height door open 31 0 cm 12 2 in 66 0 cm 26 0 in 29 1 cm 11 5 in...

Page 30: ... FA4 8 2P Fixed Angle 44 Angle rmax 66 0 mm Tube 1 8 at rmax 66 0 mm Tube 2 7 at rmax 63 0 mm Tube 3 6 at rmax 60 0 mm Tube 4 5 at rmax 59 0 mm Rotor code 4 16 602 15 847 15 093 14 841 15 000 32 200 mL B31059 B33057 FA361 5b Fixed Angle Inner row 50 Angle Outer row 30 Angle Inner row rmax 80 mm Outer row rmax 80 mm Rotor code 5 20 124 15 000 36 2 0 B31057 B33026 a Refer to the individual manuals f...

Page 31: ...e failure may generate aerosols Do not run potentially hazardous materials in this centrifuge unless all appropriate safety precautions are taken Always use the appropriate rotors and adapters Handle all infectious samples according to good laboratory practices and methods to prevent the spread of disease Ask your laboratory safety officer to advise you about the level of containment required for ...

Page 32: ...ge while it is operating Run Procedure The following detailed operating procedures are summarized at the end of this section If you are an experienced user of this centrifuge you can turn to the summary for a quick review of operating steps Preparation and Loading NOTE Before installing the rotor lubricate it following the instructions in the rotor manual 1 Check the name rating plate for the corr...

Page 33: ...ice or visit us at www beckmancoulter com CAUTION Do not drop the rotor onto the drive shaft The shaft can be damaged if the rotor is forced sideways or dropped onto it Install the rotor by centering it over the shaft and carefully lowering it straight down 7 Install the rotor according to the instructions in the rotor manual CAUTION Always run the rotor with a balanced load 8 Close the centrifuge...

Page 34: ...uired See Saving Settings Figure 2 2 Default Parameters on First Time Use Microfuge 20R shown Menu Key Sequence Pressing the MENU key starts the parameter change sequence Repeatedly pressing the MENU key steps through parameter sequence When the parameter units rpm rcf m s soft off soft stop start on soft stopon flash press or to change that parameter Theentire parameter change sequence follows th...

Page 35: ...layed The corresponding RCF is automatically calculated by the centrifuge but the RPM value is displayed during the run You can check the RCF during the run by pressing the or key while the centrifuge is running 3 Press the MENU key until all screen functions stop flashing to confirm the selection The speed setting will be saved after approximately 20 seconds if no other key is pressed within this...

Page 36: ...e setting will be saved after approximately 15 seconds if no other key is pressed within this time period To start a continuous run 1 Press the MENU key until the time unit flashes on the display Program mode ends if no other keys are pressed within 15 seconds 2 Press the cursor key until the display switches from 00 10 to After 99 minutes and 59 seconds any additional run time will no longer be d...

Page 37: ...hing of the actual temperature with the menu unit that also flashes NOTE Temperatures may vary slightly between instruments If sample temperature is crucial test temperature settings on your instrument using water samples To set the run temperature 1 Press the MENU key until the temperature unit flashes on the display The TEMP C display flashes indicating that the temperature can be entered with t...

Page 38: ... 4 Install the rotor See the specific rotor manual for complete information Precooling the centrifuge 20R refrigerated models only To maintain sample integrity during low temperature runs it is recommended to precool the empty rotor and instrument prior to the run This centrifuge has a special program that precools the centrifuge under defined conditions NOTE Once the precooling program is loaded ...

Page 39: ...r actual times of fast and soft acceleration and deceleration by rotor To activate both Soft start and Soft stop functions 1 Press the MENU key until the Soft stop start function flashes on the display Program mode ends if no other keys are pressed within 15 seconds 2 Press the or cursor key until the Soft stop start on is displayed Both soft start and soft stop functions are now activated 3 Press...

Page 40: ... display Program mode ends if no other keys are pressed within 15 seconds 2 Press the or cursor key until the Soft off is displayed Both soft functions are now deactivated 3 Press the MENU key to confirm the selection Starting a Run The run can be started using the parameters in memory from a previous run or using new or changed parameters that you enter using the procedures described above To sta...

Page 41: ...K RUN button but pressing and holding the START STOP button serves the same function When the QUICK RUN key is pressed the rotor accelerates at a maximum rate to the set speed and continues to spin as long as long as the QUICK RUN key is pressed The current run time acceleration and deceleration settings are overridden by the short run function When the QUICK RUN keyisreleased therotorbeginsdecele...

Page 42: ...uge decelerates at maximum rate to a standstill After the quick run and the centrifuge has come to a complete stop the door unlocks automatically and the program that was set beforehand is displayed again Changing Parameters During a Run While a run is in progress speed time and temperature Microfuge 20R only run parameters can be altered without stopping the run Run duration can also be changed f...

Page 43: ...ge 20R only 1 Press the MENU key until the temperature function flashes on the display The last digit on the TEMPERATURE display flashes indicating that the temperature can be raised or lowered with the cursor keys 2 Press the or cursor key until the required temperature is displayed The new parameter takes effect immediately Stopping a Run A timed run ends automatically when the TIME display coun...

Page 44: ...rifuge and accessories Summary of Run Procedures For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibrium For low temperature runs precool the centrifuge by running a 30 minute cycle with a precooled rotor installed at the required temperature with the speed set at 2000 rpm 1 Press the POWER switch to on I a Open the centrifuge door press the OPEN key a...

Page 45: ...parameters are correct and the door is shut and latched then press the START STOP key WARNING Never attempt to override the door interlock system while the rotor is spinning CAUTION Do not lift or move the centrifuge while the rotor is spinning 7 Wait for the set time to count down to zero or end the run by pressing or holding the START STOP key 8 After the rotor stops spinning press the OPEN key ...

Page 46: ... only Acceleration Deceleration Rotor type code To save settings into a program 1 Press the PROG key 2 Press the or cursor key to scroll and select an unused program number The display Prog flashes represents numbers 01 through 10 NOTE Scrolling through the programs causes the parameters of each program to display NOTE Program numbers that are already occupied will be overwritten with the current ...

Page 47: ...hing sound stops 5 Press the START STOP key To change a saved program 1 Press the PROG key 2 Press the or cursor key to scroll and select the desired program number to be changed 3 Using the MENU key and the or cursor keys change speed time temperature and or acceleration parameters 4 Press the MENU key to confirm the selections and the program The program is changed 5 Close the centrifuge door an...

Page 48: ...B30162AA 2 18 Operation Saving and Using Programs ...

Page 49: ...e SPEED display Such problems may result from incorrect input or from an equipment malfunction Refer to Table 3 1 to determine the cause of the problem and recommended actions If you are unable to correct the problem call Beckman Coulter Field Service To help diagnose and correct the problem gather as much information about the situation as you can Write down the error number that appears on the d...

Page 50: ...his time ERR and SAFE flash alternately on the display When the time is up OFF will be displayed CAUTION Do not switch the centrifuge off until the word OFF is displayed This is necessary to ensure that the rotor is at a complete standstill To clear the problem or error 1 Eliminate the source of the problem see Table 3 1 and Table 3 2 below 2 Press the door key to acknowledge and clear the error m...

Page 51: ...on I to reset Errors 34 35 and 36 will cause the centrifuge to stop Errors 37 and 38 will not cause the centrifuge to stop these errors will only returnerrormessages 40 through 45 Temperature error applies only to Microfuge 20R refrigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation 46 through 49 Imbalance error Allow to slow down Power off Allow to cool...

Page 52: ...ter error Press door key Close door Remove foreign matter from the opening of the door lock device With error 83 error message only 90 through 99 Other errors Check connections Provide sufficient water throughput only water cooled centrifuges Table 3 1 Error Message Chart Continued Error Number Problem Recommended Action Explanation ...

Page 53: ...ot closed correctly 1 Check the fuse in the mains supply 2 Plug in the power cord correctly 3 Switch the mains power on 4 Close the door Centrifuge cannot be started The displayed set speed value cannot be changed 1 Several 2 The door lock is not closed correctly 1 Cycle power If the error persists contact Beckman Coulter Field Service 2 Open and close the door If the error persists contact Beckma...

Page 54: ... exposes the operator to the possibility of electrical shock and or mechanical injury Therefore turn the power off and disconnect the instrument from the main power source by removing the Mains power plug from the outlet receptacle and refer such maintenance to qualified service personnel To gain emergency access the centrifuge 1 Turn the power switch to off O and disconnect the power cord from th...

Page 55: ...stops You should hear the door lock release See Figure 3 3 NOTE If the door is opened via the emergency door release system during a centrifuge run the centrifuge will be switched off immediately and decelerate with no brake Figure 3 3 Manually releasing the door lock with the Allen wrench 5 Reinsert the emergency access plug 1 Emergency access plug and position of the opening for the emergency do...

Page 56: ...B30162AA 3 8 Troubleshooting Accessing the Centrifuge in Case of Power Failure ...

Page 57: ...tacle and refer such servicing to qualified service personnel Do not use alcohol or other flammable substances in or near operating centrifuges WARNING Risk of personal injury or contamination If you do not properly shield yourself while performing service maintenance and troubleshooting procedures residual fluids in the instrument could injure or contaminate you Beckman Coulter recommends barrier...

Page 58: ... cleaning isrecommendedto prolong the life ofthe centrifuge Alwayscleanup spillswhen they occur to prevent corrosives or contaminants from drying on component surfaces NOTE Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed methods will not damage the equipment 1 To prevent accumulations of sa...

Page 59: ...e of service maintenance and troubleshooting procedures to avoid contact with cleaners and or residual fluids in the instrument Immediately rinse off the rotor buckets or accessories under running water if they have come into contact with any liquids that may cause corrosion Use a brush for test tubes to clean the bores of angle rotors Turn the rotor upside down and allow it to dry completely Clea...

Page 60: ...consult your laboratory safety officer regarding proper methods to use Storage and Transport Storage Before storing a centrifuge for an extended period return it to its original shipping container to protect it from dust and dirt Reinsert the shipping foam removed at time of centrifuge installation into the chamber making sure the drive shaft is stabilized in the hole in the foam Temperature and h...

Page 61: ...that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Supply List Contact Beckman Customer Service or visit www beckmancoulter com for information about ordering parts supplies and publications For your convenience a partial list is giv...

Page 62: ...B30162AA 4 6 Care and Maintenance Supply List ...

Page 63: ...r combustible fluids Vapors from these materials could enter the centrifuge air system and be ignited by the motor WARNING Maintain a 30 cm 12 in clearance envelope around the centrifuge while it is running No persons should be within this clearance boundary while the centrifuge is operating Do not handle or store hazardous materials within the 30 cm 12 in area surrounding the centrifuge Considera...

Page 64: ...ifuge weight 2 Position the centrifuge on a level surface such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge and resist vibration Referto Microfuge20NonrefrigeratedModelSpecificationsorMicrofuge20RRefrigeratedModel Specifications in CHAPTER 1 for weight a Make sure that the centrifuge front feet are fully supported on the table b Locate the centrifuge i...

Page 65: ... outlet used Refrigeration does not function properly if the frequency Hz does not match the name rating plate Plug in both ends of the centrifuge power cord If there is any question about voltage have a qualified service person measure it under load while the drive is operating A 1 8 m 6 ft power cord with grounded plug is supplied with the centrifuge Make sure that the matching wall outlet is lo...

Page 66: ...nnect the centrifuge to earth ground To preserve this safety feature Make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple outlet re...

Page 67: ...st run to ensure that the centrifuge is in proper operating condition following shipment See CHAPTER 2 Operation for instructions on operating the centrifuge After completing the test run log on to www beckmancoulter com to register the centrifuge This validates the centrifuge warranty and ensures that you receive any further information regarding new accessories and or modifications as they becom...

Page 68: ...B30162AA A 6 Installation Test Run ...

Page 69: ...terials declaration table the Table of Hazardous Susbtance s Name and Concentration and the China RoHS Caution Label are to meet People s Republic of China Electronic Industry Standard SJ T11364 2006 Marking for Control of Pollution Caused by Electronic Information Products requirements ...

Page 70: ... Assemblies O O O O O O 塑料部件 Plastic Parts O O O O O O 钣金部件 Sheet Metal O O O O O O 连接部件螺丝螺栓等 Hardware screws bolts etc O O O O O O 电源组件 Power Supply Sub Assembly O O O O O O O 表示该有毒有害物质在该部件所有均质材料中的含量均在 SJ T 11363 2006 标准规定的限量要求以下 X 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出 SJ T 11363 2006 标准规定的限量要求企业可在此处根据实际情况对上表中打 X 的技术原因进行进一步说明 O Indicates that the toxic or hazardous substances contained in all of the h...

Page 71: ...lace such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter Replacement Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be...

Page 72: ...B30162AA Warranty 2 Beckman Coulter Inc Microfuge 20 Series Centrifuge Warranty ...

Page 73: ...开电源ン電源 에 힘 Off power Aus Netzverbindung Apagado Arrêt mise sous tension Spento fuori tensione Fora de poder С сила 电源ン電源 떨어져 힘 Protective earth ground Schutzleiteranschluß Puesta a tierra de protección Liaison à la terre Collegamento di protezione a terra Terra de proteção terra Защитное заземление земля 保护接地保護アース接地 방어적인 지구 지상 Earth ground Erde Masse La tierra suelo Terre sol Scarica a terr...

Page 74: ...c All Rights Reserved www beckmancoulter com Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN 175 Available in hard copy or electronic pdf by request Available at www beckmancoulter com ...

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