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09

I

A120/130

A120/130

pH

Calibration and Measurement

This section is applicable to models A120 and A130 meters

Prior to Use

Connecting the pH Electrode

1. Take out the pH electrode from packaging. Remove the protective

cap from the bottom of electrode. If tiny air bubbles are present

inside the pH-sensitive glass membrane, gently shake the

electrode downward to remove air bubbles.

2. Place electrode into the left or right side of the electrode arm.

3. Insert BNC connector into the connector socket labeled pH/ISE.

Rotate and push the connector clockwise until it locks.

Selecting the Measurement Mode

Press the

Mode

key and the



key to select the

pH

, press the

Enter

key to confirm.

pH-sensitive glass
membrane

3M KCl solution

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Summary of Contents for A Series

Page 1: ...A series Laboratory pH ORP Ion Conductivity DO Meters USER MANUAL Bante Instruments Inc ...

Page 2: ...tion 10 Automatic Temperature Compensation 10 Manual Temperature Compensation 10 pH Calibration 11 Single Point Calibration 11 Multipoint Calibration 11 pH Calibration with Custom Buffers 12 Viewing the Calibration Log 12 pH Measurement 13 Electrode Maintenance 13 Cleaning the pH Electrode 13 Reactivating the pH Electrode 13 Storing the pH Electrode 13 Appendix 14 Preparation of pH Buffer Solution...

Page 3: ...Calibration 29 Multipoint Calibration 30 Viewing the Calibration Log 30 Measurements 31 Conductivity TDS Salinity Resistivity Measurement 31 Conductivity Ash Measurement 31 Electrode Maintenance 31 Appendix 32 Calculating the Temperature Coefficient 32 Calculating the Cell Constant 32 Calculating the TDS Conversion Factor 32 Conductivity to TDS Conversion Factors 32 Optional Accessories 32 Dissolv...

Page 4: ...This user manual provides a step by step guide to help you operate the meter please carefully read the following instructions according to the model you have purchased Environmental Conditions Before unpacking ensure that current environmental conditions meet the following requirements Relative humidity is less than 80 Ambient temperature between 0 C 32 F and 50 C 122 F No potential electromagneti...

Page 5: ... mm jack 3 Socket for 4 pole conductivity electrode 6 pin DIN 4 Socket for 2 pole conductivity electrode or dissolved oxygen electrode 6 pin DIN 5 Socket for temperature probe 3 5 mm jack 6 USB B interface to the computer or printer 7 Socket for power adapter Keypad Key Function Switch the meter on or off Return to measurement mode Select the measurement mode Press and hold the key to set the temp...

Page 6: ... swivel the electrode arm 90 Electrode holder is now ready to swing into desired position Adjusting the Electrode Arm After installation if the electrode arm automatically rises or falls you are able to adjust the screw until arm locate at any position 2 1 Remove the plastic cover from the right side of the electrode arm 2 2 Use the screwdriver to tighten the screw moderately 2 3 Insert the plasti...

Page 7: ...e user must enter a 4 digit password to access above modes If the setting value is 0000 the password protection will invalid Enable Disable Default Brightness Set the brightness level of backlight Low mid high Clear Stored Data Delete all data logs in the memory Enable Disable Default Factory Reset Reset the meter to factory default settings If enabled all of the meter settings and calibration log...

Page 8: ...word protection screen when pressing the Cal key Press the key to enter the password press the Enter key to confirm If password is correct the meter will unlock immediately If you forgot your password please contact the supplier and providing the serial number of meter Factory Reset 4 1 In the measurement mode press and hold the key to enter the setup menu 4 2 Press the key to highlight Factory Re...

Page 9: ...ting the Temperature Probe 1 1 Place the temperature probe into the circular hole located at the center of the electrode arm 1 2 Insert the jack plug to the connector socket labeled C Ensure the connector is fully seated Calibrating the Temperature Probe 2 1 Place the temperature probe in a solution with a known accurate temperature and wait for the reading to stabilize 2 2 Press and hold the C ke...

Page 10: ...air bubbles are present inside the pH sensitive glass membrane gently shake the electrode downward to remove air bubbles 2 Place electrode into the left or right side of the electrode arm 3 Insert BNC connector into the connector socket labeled pH ISE Rotate and push the connector clockwise until it locks Selecting the Measurement Mode Press the Mode key and the key to select the pH press the Ente...

Page 11: ...e current settings press and hold the key to enter the setup menu press the key to select an option or set a value press the Enter key to confirm During the setting process press and hold the key will make the value change faster To exit the setting without saving changes press the ESC key Temperature Compensation For better accuracy we recommend the use of either a sensor with a built in or a sep...

Page 12: ... entered the correct temperature value to the meter Stir the standards and samples at a uniform rate that will help you get most accurate readings Setting the Number of Calibration Points 1 Press and hold the key to enter the setup menu 2 Press the key to highlight Calibration Points press the Enter key to confirm 3 Press the key to select the number of calibration points press the Enter key to sa...

Page 13: ...to the buffer solution stir gently and wait until the measurement is stable 3 3 Press the Cal key the status bar shows Setting the calibration value 3 4 Press the key to set the value press the Enter key to begin the calibration 3 5 When the mV reading has stabilized the status bar will show Setting the calibration value again the meter prompts you to continue with second point calibration 3 6 Rep...

Page 14: ...distilled water and soak in 3M KCl solution Salt Deposits Dissolve the deposit by immersing the electrode in warm tap water Rinse the electrode with distilled water and soak in 3M KCl solution Oil or Grease Place the electrode in the detergent or ethanol solution for 15 minutes Rinse the electrode with distilled water and soak in 3M KCl solution Protein 1 Add 1 pepsin to 0 1M HCl solution 2 Place ...

Page 15: ...scription E201 BNC For general purpose applications E202 BNC For measuring the flat surface samples P11 For measuring the non high temperature liquids P11 LiCl For measuring the non aqueous samples P11 NA For measuring the biofuels P13 For measuring the micro volume samples P15 For measuring the low conductivity samples P16 For measuring the liquids with Tris buffers P18 For measuring the slurries...

Page 16: ... with strong redox potential 502 For measuring the sample with weak redox potential 504 For measuring the high temperature samples 100 C 1 Take outtheORPelectrodefrompackaging Removethe protective cap from the bottom of electrode 2 Place electrode into the left or right side of the electrode arm 3 Insert BNC connector into the connector socket labeled pH ISE Rotate and push the connector clockwise...

Page 17: ...rode into the standard solution stir gently and wait until the measurement is stable 1 2 Press the Cal key the status bar shows Setting the calibration value 1 3 Press the key to set the value Press the Enter key the meter begins the calibration 1 4 Wait for the mV reading to stabilize the meter will automatically show Calibration is completed and return to the measurement mode To exit the calibra...

Page 18: ...page 42 Electrode Maintenance Rinse the ORP electrode thoroughly with distilled water after use In the corrosive chemicals viscous solutions and solutions with heavy metalsor proteins take readingsquicklyand rinseelectrode immediately If the electrode response becomes sluggish refer to the instructions below to clean the electrode 1 Inorganic Deposits Place the electrode in 0 1M HCl solution for 1...

Page 19: ...olve 3 grams of quinhydrone reagent in 500 ml of the pH 7 00 buffer solution stir the solution for 10 minutes Undissolved quinhydrone reagent must be present If necessary add the reagent Temperature Potential 10 mV 20 C 94 mV 25 C 87 mV 30 C 80 mV Due to the quinhydrone solution is susceptible to air oxidation in storage make sure to prepare the fresh solution before use Preparation of Electrode S...

Page 20: ...of electrode Soak the electrode in a 100 ppm standard solution water hardness electrode in a 10 mmol L standard solution for about 10 minutes 1 2 Place electrode into the left or right side of the electrode arm 1 3 Insert BNC connector into the connector socket labeled pH ISE Rotate and push the connector clockwise until it locks Selecting the Measurement Mode 2 1 Press the Mode key and the key to...

Page 21: ...gree f French degree mg L CaCO3 mg L CaO mg L Ca2 mmol L Calibration Points Set the number of calibration points 2 to 5 points Default 2 points Ionic Valency Set the valence of the ion selective electrode Monovalent Default Divalent Alarm Limits Set the high and low limit values to activate alarm Enable setting range 0 to 30000 Disable Default Calibration Due Set the calibration interval to activa...

Page 22: ...owing options Measurement Unit Calibration Points ppm mg L 0 001 0 01 0 1 1 10 100 1000 10000 mol L mmol L 0 001 0 01 0 1 Before beginning the calibration ensure that the ionic valency option in the setup menu matches connected electrode All of the standards and samples should be at the same temperature and calibration points cover the anticipated range of the samples For the low concentration or ...

Page 23: ...illed water then rinse with a small amount of standard solution Place the electrode and temperature probe into the next standard solution and stir gently 8 Press the Enter key the meter begins the calibration 9 When the mV reading has stabilized the screen will show the next calibration point 10 Repeat the steps 7 and 8 above until the meter returns to the measurement mode Calibration is completed...

Page 24: ...n rinse with a small amount of standard solution Place the electrode and temperature probe into the standard solution stir gently to create a homogeneous solution 5 Press the Enter key the status bar shows Calibrating 6 When the mV reading has stabilized the screen will show Calibration Point 100 mmol L the meter prompts you to continue with second point calibration 7 Repeat the steps 4 and 5 abov...

Page 25: ...electrode with distilled water During the measurement process never wipe the ion sensitive membrane blot dry with a lint free tissue to remove waterdrops on electrode To record the measurement at the predefined time intervals refer to the Interval Readings section on page 42 Known Addition 2 1 Press the Mode key and the key to select the Ion Known Addition press the Enter key to confirm 2 2 Press ...

Page 26: ... L 4 7 Press the key to enter the value press the Enter key to confirm When the setting is completed the meter will calculate and show the sample addition result 4 8 Press the ESC key to take a measurement again Sample Subtraction This method is similar to the known subtraction method except that the sample is added to the standard solution 5 1 Press the Mode key and the key to select the Ion Samp...

Page 27: ...ammonium standard 480 ml ION Br 1000 ppm bromide standard 480 ml ION Cd 1000 ppm cadmium standard 480 ml ION Ca 1000 ppm calcium standard 480 ml ION Cl 1000 ppm chloride standard 480 ml ION Cu 1000 ppm cupric standard 480 ml ION F 1000 ppm fluoride standard 480 ml ION I 1000 ppm iodide standard 480 ml ION Pb 1000 ppm lead standard 480 ml ION NO3 1000 ppm nitrate standard 480 ml ION K 1000 ppm pota...

Page 28: ...e electrode for about 10 minutes in tap water to remove dirt and oil stains on the sensor surface 2 Place electrode into the left or right side of the electrode arm 3 Insert 6 pin connector into the connector socket labeled EC DO Ensure the connector is fully seated Selecting the Measurement Mode Press the Mode key and the key to select the conductivity TDS salinity or resistivity mode press Enter...

Page 29: ...ing range 0 0 to 10 0 C Default 2 1 C Non linear Pure Water Coefficient The pure water coefficient is used to correct the sample solution with a conductivity of less than 5 µ S cm If enabled the meter will be automatically calculated and applied coefficient for ultra pure water measurement Enable Disable Default Reference Temperature Set the normalization temperature for measurement the readings w...

Page 30: ...conductivity electrodes Before the calibration and measurement ensure that you have selected a suitable electrode according to the anticipated sample conductivity The following table lists the selectable electrode and its effective measurement ranges Electrode Measurement Range Cell Constant CON 0 1 0 5 to 100 µ S cm K 0 1 CON 1 10 µ S cm to 20 mS cm K 1 CON 10 100 µ S cm to 200 mS cm K 10 If the ...

Page 31: ...ion in the setup menu When the first calibration point is completed the screen will show Calibration Point 2 the meter prompts you to continue with second point calibration 2 2 Rinse the conductivity electrode with distilled water then rinse with a small amount of standard solution 2 3 Repeat the steps 1 4 and 1 5 above until the meter returns to the measurement mode Calibration is completed Perfo...

Page 32: ...lected ICUMSA method 2 2 Press the Mode key and the key to select the either Refined Sugar or Raw Sugar measurement mode 2 3 Press the Enter key the screen shows an input window and waits for entering the conductivity of the used water for preparing sugar solution range 0 0 to 100 0 µ S cm 2 4 Press the key to set the value press the Enter key to confirm When the setting is completed the meter wil...

Page 33: ... the reading 3 Calculate the cell constant using the following formula K Cstd Cmeas G Where K Cell constant Cstd Value of conductivity standard solution Cmeas Measured value G Raw cell constant 0 1 1 or 10 Calculating the TDS Conversion Factor To determine the TDS factor of sample solution use the formula below Factor Actual TDS Actual Conductivity 25 C Where Actual TDS value from the high purity ...

Page 34: ... membrane cap halfway with electrolyte solution 1 3 Screw membrane cap back onto the electrode Some electrolyte solution will overflow during this process 1 4 Check the electrode ensure that no air bubbles are trapped in the electrolyte solution and the membrane is not creased or damaged Connecting and Polarizing the Electrode 2 1 Place electrode into the left or right side of the electrode arm 2 ...

Page 35: ...rm Enable setting range 0 00 to 20 00 mg L Disable Default Calibration Due Set the calibration interval to activate alarm Enable setting range 1 to 31 days Disable Default If you want to change the current settings press and hold the key to enter the setup menu press the key to select an option or set a value press the Enter key to confirm During the setting process press and hold the key will mak...

Page 36: ...ration in mg L or ppm Mode 1 1 Ensure that the meter is in DO mg L mode and you have select the 1 point calibration in the setup menu 1 2 Press the Cal key the screen shows Calibration point 8 26 mg L 1 3 Place the dissolved oxygen electrode into the air saturated water for 10 minutes and stir gently Press the Enter key to begin the calibration 1 4 Wait for the dissolved oxygen reading to stabiliz...

Page 37: ...p menu 4 2 Press the Cal key and the key the meter shows Calibration Point 0 0 4 3 Place the electrode into the zero oxygen solution for 10 minutes and stir gently Press the Enter key to begin the calibration 4 4 When the dissolved oxygen reading has stabilized the screen will show Calibration Point 100 0 the meter prompts you to continue with second point calibration 4 5 Place the electrode into ...

Page 38: ...enabled the meter will automatically sense a stable reading and lock measurement the Hold icon appears on the screen Press the key to resume measuring If the option is disabled the meter will continuously measure and update the readings To record the measurement at the predefined time intervals refer to the Interval Readings section on page 42 BOD Measurement The A180 meter contains a BOD measurem...

Page 39: ...g with the data log 0000 to 9999 Default 0000 Sample Volume Set the sample volume Default 100 ml Total Volume Set the total volume of sample Default 100 ml Minimum Testing Time Set the minimum time of measurement When the minimum time is reached the measurement will start Default 1 minute Maximum Testing Time Set the maximum time of measurement When the maximum time is reached the measurement will...

Page 40: ...t to correct value In the OUR mode this parameter must be set to 1 0 If you want to exit the OUR and SOUR measurements press the Mode key to convert the measurement mode Electrode Maintenance Rinse the dissolved oxygen electrode thoroughly with distilled water after use Do not touch the membrane and always keep it is clean and wet If you do not use the electrode for long periods screw off the memb...

Page 41: ...A180 181 I 40 Communication and Power Supply Order Code Description USB 2303B USB connector A to B 1 m 3 3 ft cable DCPA 12V 12V DC power adapter european standard plug ...

Page 42: ...ent press the MI key to store the reading into the memory the screen shows Measured value has stored Viewing the Data Logs 1 1 Press the MR key and the key to select the Stored Data 1 2 Press the Enter key the screen shows a measurement data list 1 3 If necessary press the key to switch pages 1 4 Press the ESC key to return to the measurement mode If the meter is not store any data the screen will...

Page 43: ...t on your computer 3 Click the OK the application starts 4 Click the Connect the screen shows Port is connected 5 Click the OK then click the Receive the stored data will transfer to computer automatically If your computer can not find a communication port click the PL2303_ Prolific_DriverInstaller_V1190 exe to update the drive program Creating an Excel File When transfer is completed click the Sa...

Page 44: ...tion 0 1 mV Accuracy 0 2 mV Calibration Point 1 point only for ORP mode Ion Concentration Range 0 001 to 30000 ppm mg L mol L mmol L Resolution 0 001 0 01 0 1 1 Accuracy 0 5 F S monovalent 1 F S divalent Calibration Points 2 to 5 points Calibration Solutions 0 001 0 01 0 1 1 10 100 1000 10000 Temperature Compensation 0 to 100 C 32 to 212 F Water Hardness Range 0 05 to 200 mmol L Resolution 0 01 0 ...

Page 45: ... Conductivity Ash Range 0 00 to 100 Resolution 0 01 0 1 1 Accuracy 1 F S Measurement Modes Refined sugar or raw sugar Dissolved Oxygen Range 0 00 to 20 00 mg L 0 0 to 200 0 saturation Resolution 0 01 Accuracy 0 2 mg L 2 0 Calibration Points 1 or 2 points Temperature Compensation 0 to 50 C 32 to 122 F Pressure Correction 60 0 to 112 5 kPa 450 to 850 mmHg Salinity Correction 0 0 to 50 0 g L Temperat...

Page 46: ...for meter is one year from the date of shipment Above warranty does not cover the electrodes and standard solutions Out of warranty products will be repaired on a charged basis The warranty on your meter shall not apply to defects resulting from Improper or inadequate maintenance by customer Unauthorized modification or misuse Operation outside of the environment specifications of the products For...

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