BANDELIN SONOCOOL 255, User Instructions, Page: 24 / 46

24 / 46 51346-007 GB/2020-08
1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com
4.3 Ultrasound operation
For optimal di ff usion of the ultrasound and protection of the oscillating tank, nothing should
be in contact with the bottom of the bath during sonication. Laboratory fl asks and other
sample vessels must be stored in the supplied beaker holder or in a similar accessory.
• It is possible to treat multiple sample vessels with di
ff erent fl uids at the same time.
• When treating small quantities of combustible fl uids in sample vessels, the country-
speci fi c guidelines/regulations that are currently in e ff ect are to be observed.
The ultrasonic bath is turned o ff for inserting the beakers.
 Insert the inset beakers into the beaker holder, making sure that:
When using the SONOCOOL for sample
preparation for analysis, we recommend using
only the inner drilling holes marked green and
orange for the sample vessels, in order to achieve
a uniform sonication.
• The inset beakers are immersed a minimum of
2 cm in the contact liquid.
• Possible air bubbles are completely removed from
below the vessels.
• When using sample containers, e.g. sample cassettes
for decalci fi cation of bone tissue, make sure of the
following:
- All samples must be fully covered with fl uid.
- If sample cassettes are stacked in the glass, this
may signi fi cantly impair the distribution of sound.
- There may not be any air bubbles in hollow areas (e.g. blind holes).
After inserting the beakers and beaker holders, the fi ll level of the oscillating tank must
always be at or slightly above the fi lling level mark. A fi ll level that is too low will
damage the ultrasonic bath.
 Set the desired sonication time and start the ultrasound, see chapters 3.4 and 3.5.
 Following sonication, remove the beaker holders with inset beakers from the tank and
set down on a level surface.
 Before the next sonication, verify the service life of the contact fl uid. Heed the
speci fi cations of the preparation manufacturer. If the fl uid is heavily contaminated, it
should be replaced even earlier as it can reduce the e ffi cacy of the ultrasound.

Power o ff the ultrasonic bath ( mains switch on the rear side).
The last-selected parameters ( runtime, target temperature, target power) will be saved
and accessed the next time the ultrasonic bath is started.
PH 255-11 for analysis
2/
3
≥
2c
m
Inset beakers SD 01.2 made of glass
Contact liquid Sample
(reagent)