BANDELIN SONOCOOL 255, User Instructions, Page: 22 / 46

22 / 46 51346-007 GB/2020-08
1 electronic GmbH & Co. KG • Heinrichstraße 3-4 • 12207 Berlin • Deutschland • info@bandelin.com
4 Use
Depending on the application, the samples are sonicated indirectly in laboratory fl asks or
other sample vessels, which are placed on special holders and inserted into the contact
fl uid in the oscillating tank.
Direct sonication of objects in the bath fl uid is not permitted with
the SONOCOOL.
The intended process sequence is described step by step in the
following chapters.
4.1 Fill the oscillating tank and de-air it
In order to transmit the ultrasound between the oscillating tank and the laboratory fl asks,
the tank must be fi lled with a contact liquid composed of normal municipal or deionised
water together with a surfactant, e.g. TICKOPUR TR 3 . For additional information, see
chapter 4.5.1.
The ultrasonic bath is turned o ff for fi lling.
CAUTION!
In order to achieve full de-airing and thus proper liquid circulation, the following
steps must absolutely be adhered to in the given sequence.
 Checking that the emptying shut-o ff valve is closed.
 For initial fi lling, remove the rubber stoppers from
the tank's outlet (bottom). Leave the upper stoppers
(in the inlet) in place without fail.
 First fi ll the oscillating tank approx. 1/3 full with
water , then add 50 ml TICKOPUR TR 3. Next, add
water up to 2 cm above the
fi lling level mark.
 Power on the ultrasonic bath using the mains switch
on the rear side. After it is turned on, the LC display
must light up. The internal pump starts operating.
 Remove the rubber stoppers from the inlet.
 The contact liquid must be visibly and palpably fl owing into the tank through the inlet.
The temporary appearance of air bubbles after the liquid has been fi lled is normal.
Note:
In rare cases in which liquid is not conveyed, it is possible that an air bubble has
formed in the system and is preventing the conveyance of liquid through the pump. In
such a case, a portion of the liquid must be drained. The ultrasonic bath must be turned
on during this process. To do so, remove the protective cap from the drain, attach the
hose supplied with delivery, and open the shut-o
ff valve. Here, the water level must
still be above the inlet. Circulation gets underway and it is possible to clearly see how
the liquid
fl ows out at the inlet. Afterwards, fully re fi ll the ultrasonic bath (with the liquid
collected) .
 If the level of the liquid drops signi fi cantly below the fi lling level mark, water must be
Inset beakers in the beaker holder
Contact liquid Sonication fl uid
Rubber stoppers in the outlet
Rubber stoppers in the inlet