Ankom XT4, Operator'S Manual

Page: 20 / 28

Operator’s Manual
pg. 20 Rev 5/21/18
16. Press START on the Keypad. The ANKOM
HCl
Hydrolysis System will automatically heat and maintain
the set temperature. After hydrolysis is complete, the samples are automatically rinsed with cold water.
When the instrument has completed the automated process, the Display will show “Process Complete”.
17. When the automated process is complete, open the Lid Latch and immediately wipe the PTFE pad on the
underside of the lid with paper towel.
This step is extremely important to prevent acid from corroding the hinge
on the Vessel Lid.
18. Remove the Multi-bag Holder from the Hydrolysis Vessel.
19. Remove the individual filter bags from the Multi-bag Holder and place
them on four layers of paper towel.
20. Place two layers of paper towel on top of the wet filter bags. Using the
ANKOM Blotter, apply uniform pressure to the top of the paper towels
for two minutes to gently press the water out of the filter bags. Blotting
helps to reduce the drying time and the amount of acid residue in the
bags.
21. Repeat the blotting process for two more minutes before drying.
22.
Completely dry the samples in an ANKOM
RD
Dryer set to 110
°
C for three hours. This will ensure that the
temperature inside the bags is at 100 - 105°C. (An oven resistant to HCl fumes can be used if it can ensure
that the temperature inside the bags is at 100 - 105°C.)
Residual acid left in the samples due to incomplete drying will cause
corrosion in the extractor and artificially high fat values.
23. Remove samples from the dryer/oven and place them directly into a Desiccant Pouch containing pH
paper.
24. Allow samples to cool to room temperature and check the pH paper. If the presence of acid shows on the
paper, continue drying. If the pH paper is neutral, record weight (W
2
) of samples.
If your hydrolysis results are low, it is possible that the acid was not able to
completely saturate and hydrolyze the sample. In this case, it may be
necessary to use no more than 1.0g of the combination of sample and DE in
your filter bags. Depending on the sample content, a possible combination
could be 0.5g of sample and 0.5g of DE.
Your samples are now ready for fat extraction in an ANKOM
XT15/XT10
Extractor. See the specific extractor
Operator’s Manual for instructions.