Operator’s Manual
Rev 5/21/18
pg. 19
Hydrolysis step-by-step procedure using the
ANKOM
HCl
Hydrolysis System
To perform hydrolysis on prepared samples, follow the procedure detailed below.
1.
Turn the instrument power switch ON.
2.
Turn the cold water supply on.
3.
Pull the Lid Latch up to open the Vessel Lid.
4.
Place the Multi-bag Holder into the Hydrolysis
Vessel.
5.
Pour 500ml of 3N HCl into the Hydrolysis
Vessel to cover the ports in the back.
6.
Roll up a piece of paper towel and place it on
the Vessel Lid just above the Hinge as shown
to the right. This will help prevent drips of
liquid from getting on the Hinge after a run.
7.
Push the Lid Latch down to close the Vessel Lid.
8.
Press ENTER on the Keypad.
9.
Use the UP/DOWN arrows on the Keypad to enter the hydrolysis time (suggested time is 60 minutes).
10.
Press ENTER on the Keypad.
11.
Use the UP/DOWN arrows on the Keypad to enter the hydrolysis temperature (suggested temperature is
90°C).
12.
Press ENTER on the Keypad.
13.
Use the UP/DOWN arrows on the Keypad to enter the rinse time (suggested time is 20 minutes).
14.
Press ENTER on the Keypad.
15.
Make sure the Vessel Lid is closed (a message on the Display will remind you to do this).
Display
Keypad
Lid Latch
Vessel Lid
Hinge on
Vessel Lid
Hydrolysis
Vessel
Rolled-up
Paper Towel
Ports in back
of Vessel
Summary of Contents for XT4
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