Analytik Jena qTOWER3 84 Operating Manual Download Page 1

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Operating manual

qTOWER

84 / qTOWER

84 G

Real-Time PCR Thermal Cycler

Summary of Contents for qTOWER3 84

Page 1: ...Operating manual qTOWER3 84 qTOWER3 84 G Real Time PCR Thermal Cycler ...

Page 2: ... Str 1 07745 Jena Germany Phone 49 3641 77 9460 Fax 49 3641 77 9279 Email bio application analytik jena de http www analytik jena com Microsoft and Windows are registered trademarks of Microsoft Corp The identification with or TM is omitted in this manual 10 3107 072 23 02 19 Analytik Jena AG Copyright 2019 Analytik Jena AG Manufacturer Technical Service Application Support General Information Cop...

Page 3: ...plosion and fire 11 2 6 3 Safety instructions electrical equipment 11 2 6 4 Handling of samples auxiliary and operating materials 12 2 6 5 Safety instructions service and repair 13 2 7 Behavior during emergencies 14 2 8 Standards and directives 15 3 Technical data 17 4 Installation conditions 19 4 1 Environmental conditions 19 4 2 Energy supply 19 4 3 Space requirement 20 5 Function and setup 21 5...

Page 4: ...rn 41 10 4 Moving the qTOWER 84 in the laboratory 41 10 5 Storage 42 11 Disposal 42 12 Index 43 Figures Fig 1 Layout of the qTOWER 84 21 Fig 2 Schematic structure of the Epi fluorescence photometer 22 Fig 3 qTOWER 84 front view 24 Fig 4 qTOWER 84 open 24 Fig 5 qTOWER 84 rear view 25 Fig 6 Type plate 26 Fig 7 Voltage selector switch on the bottom of the qTOWER 84 26 Fig 8 Mains and connection cable...

Page 5: ...as itemized lists sub listings as bullet points Safety notes are indicated by pictographs and signal words The type and source of the danger are stated together with notes on preventing the danger The meaning of the pictographs and signal words used is explained in section Symbols and signal words used p 6 The elements of the control and analysis program are indicated as follows Program terms are ...

Page 6: ...vice control and monitoring User administration Context sensitive help functions Design of real time PCR experiments and their evaluation Storage of methods templates and measuring results projects Planning and evaluation of Absolute quantifications Relative quantifications ΔΔCt analyses DNA melting curves Genotyping End point analyses Results printout Results export to MS EXCEL or as CSV file Res...

Page 7: ...d liability claims are excluded for personal injury and property damage due to one or several of the following causes use of the qTOWER 84 other than intended improper commissioning operation and servicing of the device modifications of the equipment without prior consultation with Analytik Jena AG unauthorized intervention in the equipment operation of the device with faulty safety equipment use ...

Page 8: ...death or most serious injuries incapacitation can result CAUTION Indicates a potentially hazardous situation Unless avoided light or minor injuries may result CAUTION Hot surface Touching the hot surface can cause burns WARNING Danger of electric shock if touched Electrical voltages present a danger of electrical shock BIOLOGICAL HAZARD This symbol indicates that precautions must be taken when wor...

Page 9: ... of which language they speak CE marking Manufacturer of the product including address Manufacturing date of the product Order number of the product Temperature limit Serial number of the device Observe operating instructions Caution In combination with the symbol above Attention Note the accompanying documents Symbol for waste disposal in accordance with WEEE directive Do not dispose of in househ...

Page 10: ...following notice sign is attached to the rear of the qTOWER3 84 2 3 Technical condition The qTOWER 84 corresponds in its design and construction to the current state of the art technology Unauthorized modifications or changes especially such that affect the safety of the staff and the environment are generally not allowed Observe the following Any manipulation of the safety equipment is prohibited...

Page 11: ...device The op erating personnel must be familiar with the dangers arising from samples and excipients Suitable personal protection equipment must be worn Prior to pauses or at the end of the work appropriate skin cleaning and protec tion measures must be carried out Eating drinking and smoking at the location of the device is prohibited The device must be handled with the appropriate care and kept...

Page 12: ...pment Since the device does not have handles grip the device firmly with both hands at the lower end lifting it simultaneously The guide values and statutory limits for lifting and carrying loads without auxiliary equipment must be observed and adhered to The qTOWER 84 can be installed by the Analytik Jena AG customer service or trained specialist personnel authorized by Analytik Jena AG 2 6 Safet...

Page 13: ...hat are suitable for high temperatures up to 100 C fit well into the thermal block no shaking and whose lids seal tightly 2 6 2 Safety instructions Protection against explosion and fire The qTOWER 84 must not be operated in an explosive environment The operating personnel must be familiar with the location of the fire fighting equipment in the operating room of the device The qTOWER 84 must not be...

Page 14: ...ielded deliberately operated high frequency source as these may affect proper operation of the device 2 6 4 Handling of samples auxiliary and operating materials The operator is responsible for the selection of substances used in the process as well as for their safe handling This is particularly important for radioactive pathogenic infec tious poisonous corrosive or otherwise dangerous substances...

Page 15: ...risk of the vessels being damaged and sample liquid leaking out For your own safety please observe the potential infectious qualities of the ex amined biological material 2 6 5 Safety instructions service and repair On principle maintenance of the qTOWER 84 is performed by the customer service department of Analytik Jena AG or its authorized and trained specialist personnel Un authorized maintenan...

Page 16: ...following has to be ensured The operating staff must be familiar with the location of safety equipment acci dent and danger alarms as well as first aid and rescue equipment as well as their handling The operator is responsible for the respective training of the operating staff All equipment for first aid first aid kit eyewash bottles stretcher etc as well as equipment for firefighting fire extingu...

Page 17: ...ference suppression and interference resistance and meets the requirements of EN 61326 1 2013 Interference resistance EN 61326 1 2013 Interference emission The qTOWER 84 complies with the applicable requirements of EMC directive 2014 30 EU and low voltage directive 2014 35 EU Environmental compatibility The qTOWER 84 has been tested for environmental compatibility and meets the re quirements of DI...

Page 18: ...riction of the Use of Hazardous Substances in Electrical and Elec tronic Products Analytik Jena guarantees that those hazardous substances may not leak out during the next 25 years when the instrument is used in accordance with its intended purpose The device leaves the manufacturer in a pristine and technically safe state To ensure safe operation the operator must strictly observe the safety and ...

Page 19: ... C Temperature increments min 0 1 C cycle Time increments min 1 s cycle Heated lid Manual opening mechanism automatic contact pressure Heated lid contact force comply with 30 kg automated Dimensions H x W x D 59 cm x 27 5 cm x 27 5 cm 70 cm x 27 5 cm x 50 cm when opened Mass 30 kg Noise level 45 dB Energy supply Operating voltage Line frequency 100 V 115 V 230 V 50 60 Hz Power consumption max max ...

Page 20: ...Color module 2 515 nm 545 nm Color module 3 535 nm 580 nm Color module 4 565 nm 605 nm Color module 5 630 nm 670 nm Color module 6 660 nm 705 nm Protein color module 1 490 nm 580 nm FRET filter combinations FRET module 1 470 nm 580 nm FRET module 2 470 nm 670 nm FRET module 3 470 nm 705 nm FRET module 4 515 nm 670 nm FRET module 5 470 nm 605 nm Software and PC qPCRsoft384 Control and analysis prog...

Page 21: ... not locate the device directly near a door or window Place the device on a heat resistant and acid resistant surface Do not locate the device near sources of electromagnetic interference Avoid direct sunlight and radiation from heaters onto the qTOWER 84 if nec essary ensure air conditioning Never cover the air vents of the backplate of the device with other equipment or installations 4 2 Energy ...

Page 22: ...e qTOWER 84 the upper device hood tilts back Allow adequate space for this qTOWER3 84 Height cm Width cm Depth cm closed 59 27 5 27 5 open 70 27 5 50 The open device has a space requirement of 70 cm x 27 5 cm x 50 cm H x W x D Allow adequate space for placement of the device In addition keep a safety distance of at least 10 cm to other equipment or walls Additional space may be required next to th...

Page 23: ...r with fiber multiplexer and mechanical scan ning device is used as detector Light for exciting the dyes is emitted by four color LED in blue green white and red The light is passed through optic fibers to collimator lenses bundled and then trans ferred to the excitation filter of the color modules fitted to a rotating filter wheel The light is deflected via a beam divider and passed through addit...

Page 24: ...omultiplier CPM for detection The filter wheel of the photometer can be populated freely with a choice of color filter modules In addition color filter modules can be retrofitted at any time extending the application spectrum of the device A total of 12 color filter modules are available This also includes filter modules especially optimized for FRET applications You can chose color modules from a...

Page 25: ...s The qTOWER 84 G also features a 384 well thermal block but is additionally equipped with a gradient function The maximum temperature span of the gradient is 24 C with 24 columns This means that the device is particularly well prepared for establishing new primary pairs To avoid condensation or potential sample loss the qTOWER 84 is equipped with a motorized heated lid This can be adjusted up to ...

Page 26: ... fulfilled at the installation loca tion see section Installation conditions p 19 6 1 Connections and control elements 1 Upper part with fluorescence spectrometer 2 Lock with handle 3 LED for status display 4 Lower part with thermal cycler Fig 3 qTOWER 84 front view 1 Heated lid 2 Sample block 3 Twistlock Fig 4 qTOWER 84 open ...

Page 27: ...be folded back from the handle The mains connection mains switch and USB interface for PC connection are on the rear of the device 1 Ventilation for fluores cence spectrometer 2 Ventilation grille at the thermal cycler 3 Service connection 4 Mains connection 5 Fuse holder for instru ment fuses 6 Mains switch 7 Type plate 8 USB port for PC con nection Fig 5 qTOWER 84 rear view The type plate is als...

Page 28: ...nd instrument fuses 9 Serial number 10 Order number 11 Device type and model Fig 6 Type plate The voltage selector switch is located on the bottom of the device behind a cover It can be used to adjust the operating voltage to the mains voltage Fig 7 Voltage selector switch on the bottom of the qTOWER 84 The following accessories are part of the qTOWER3 84 Mains and connection cable CD or USB memor...

Page 29: ...suitable packaging material may cause damage to the device Keep the original packag ing for subsequent transports The following steps are required during the installation of the qTOWER 84 Assemble the color modules unless installed Connect the device to the mains and the PC Install the qPCRsoft384 program on the PC 1 Remove the qTOWER 84 connection cable and operating manual with the in stallation...

Page 30: ... switch During first startup the qTOWER 84 will be detected as a USB device After suc cessful automatic driver installation the qTOWER3 84 is ready for operation If the drivers are not installed automatically you can complete the installation via the Windows routine The drivers are on the installation CD or on the USB memory stick 10 Start the qPCRsoft384 program 11 Open the lid To do so press in ...

Page 31: ...NTIFICATION menu command The qTOWER 84 is ready for operation The green status LED lights up Status LED The status LED is located on the front of the device next to the device name 3 in Fig 3 p 24 During the device initialization the LED flashes red green As soon as the device is ready for operation the LED switches to green During a measurement the LED flashes red green In case of a device error ...

Page 32: ...e PCR plates with 384 wells These and other consumables can be ordered from Analytik Jena AG After pipetting the PCR samples into the wells these must be sealed with a transparent optical adhesive film sealing film The optical transparency of the films affects the fluo rescence signal directly Therefore only use clear adhesive film as offered for real time PCR 1 Prepare a real time PCR project wit...

Page 33: ...ER3 84 G Edition 02 19 31 Fig 9 Position A1 on the sample block 4 Close the lid To do so fold the lid forward and press it down from the handle until the lock engages with a click 5 Start the PCR run in the qPCRsoft384 program ...

Page 34: ... limits warranty entitlements If the device exhibits any faults or details please contact the Analytik Jena AG customer service immediately In order to guarantee sound and safe operation and to ensure laboratory certification we rec ommend concluding a maintenance agreement with regular device validation 8 1 Cleaning the housing NOTICE The use of alcohol organic solvents or abrasives can damage th...

Page 35: ...TOWER 84 is used for the analysis of infectious material great care must be taken because the qTOWER 84 cannot be decontaminated as a whole device Immediately remove visible contamination by suitable means Do not allow sol vents to enter the device The sample block is also suitable for wipe and spray disinfection The only suit able cleaning method for the housing is wipe disinfection Device part r...

Page 36: ...sk of fire injuries and device damage Mains voltage Instrument fuses 100 V 115 V 230 V 2 x 10 AT 250 V 1 Switch the qTOWER3 84 off Unplug the power cord 2 Carefully open the fuse holder on the rear of the device Use a standard screwdriv er to do so 3 Remove the old fuses and replace them with new ones Fig 10 Fuse holder on the rear of the device If the fuses melt repetitively have the qTOWER3 84 c...

Page 37: ...be removed for this purpose NOTICE Sensitive optics Do not remove the interior hood because there are underneath sensitive optical elements Proceed as follows 1 Switch off the qTOWER 84 2 Open the lid Remove the four external screws on the inside of the lid 3 Close the lid 4 On the device backplate remove the four external screws at the upper part with which the hood is attached 5 Pull the hood sl...

Page 38: ... of the color module Note The correct position of the color module is found quicker if you rotate the color module slightly around its own axis during insertion Press the color module until it rests flat on the rotor 8 Screw on the color module with the M2 screws supplied 9 Repeat this procedure for all color modules 10 Close the remaining openings of the rotor with the color module dummy ele ment...

Page 39: ...correct position simply by tighten ing it further The sensitive optics may be damaged 12 Place the hood back onto the device 13 First tighten the four external screws at the upper part on the backplate of the qTOWER 84 14 Open the lid and remove the four ex ternal screws on the inside of the lid 15 Close the lid again 16 Switch on the qTOWER 84 Start the qPCRsoft384 program 17 Select the command E...

Page 40: ...ndow A new color module with the name COLOR 000 000 00 0 is created Enter the name position and properties of the color module on the right hand side of the menu window Remove a color module as follows Select the color module from the COLOR MODULES list Click on DELETE The properties of a color module can be changed as follows 1 Activate the PROPERTIES checkbox 2 In the POSITION list select the po...

Page 41: ...tions Error code Cause x 100 Device error in the optical unit of the fluorescence photometer 99 x 10 Error in the qPCRsoft384 program e g in the settings or data communication error 9 x 2 General device error e g lid open 1 No error x 0 Device error in the PCR thermocycler The following errors can be fixed by the customer Error code Cause Remedy Device cannot be activated Check voltage supply Exch...

Page 42: ...t damage from impact or vibration The device should be transported in such a way that major temperature fluctuations are avoided and the formation of condensate is thus prevented 10 2 Placing the transport lock Before packing the qTOWER3 84 the transport lock for the upper part that contains the fluorescence photometer must be installed If the transport lock is no longer available you can place an...

Page 43: ...aging is no longer available please contact Analytik Jena AG or your local distributor Please attach the warning note CAUTION SENSITIVE ELECTRONIC DEVICE to the packaging Please include a sheet containing the following data Name and address of the sender Name and telephone number of a contact for inquiries A detailed description of the fault the precise conditions and situations under which the fa...

Page 44: ...r prolonged pe riods it should be stored in its original packaging A suitable desiccant should be added to the equipment to prevent damage from moisture The following requirements are placed on the climatic conditions in the storage room of the qTOWER 84 Temperature range 10 C to 55 C Max humidity 10 to 30 11 Disposal The operator of the qTOWER 84 must dispose the waste materials that occur during...

Page 45: ...xchanging fuses 34 F Fluorescence spectrometer 21 Function 21 G Guidelines 16 H Humidity 19 I Insert samples 30 Installation site 19 Installing color modules 35 Interfaces 25 L Layout 21 Liability 5 M Mains connection 19 25 Maintenance 32 O Operating temperature 19 P PCR thermocycler 23 Personnel 9 Protection type 15 R Rating plate 25 Return 41 S Safety class 15 Safety instructions 6 electrical eq...

Page 46: ...02 19 qTOWER3 84 qTOWER3 84 G Symbols Device 8 Manual 6 T Technical data 17 Temperature Operation 19 Storage 19 Transport 40 Placing the transport lock 40 U Use 3 User manual conventions 3 V Ventilation 25 W Warranty 5 ...

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