AmScope MU USB2.0, User Manual

Page: 140 / 147

140
MU Series (Windows)
User’s Manual
Options Menu Functions
Calibration Menu
The “Calibration” options allows the user to input a rule for the software to use to determine how much of a
unit of real distance is equivalent to how many pixels the camera sees, at a given magnification. These rules are
used to perform calculated measurements while using the camera and software, giving much needed flexibility
for measuring small features in any type of a microscope. Note that this process must be repeated for every
magnification (and ideally, every unit of measurement) that the user intends to measure at. Using a setting at
a different magnification than calibrated will cause massive discrepancies between actual distance and mea -
sured distance.
1. Begin by switching the magnification on your microscope to your desired calibration setting, and placing a
unit of known measurement on the stage. The smaller the division, the more accurate the measurement func -
tions will be, down to 0.01mm (the smallest guaranteed accuracy level of the camera and software).
2. Focus on the micrometer slide or ruler while looking at the live feed. Once focused and set, adjust the live
resolution to the highest available setting, set the zoom to 100%, and the unit of measurement to pixel, in the
upper toolbar.
3. Proceed to the “Options” menu, and hit “Calibration.” The above “Calibrate” menu will appear, with a red
line overlayed on the image. Adjust the line to position it on the 0 point of your ruler, and the first unit of mea -
surement (such as 1mm if using a ruler, or 0.01mm if using a micrometer slide), so that the line spans between
the two points.