AmScope MU USB2.0, User Manual

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MU Series (Windows)
User’s Manual
Process Menu Tools
Image Stacking
“Image Stacking” is a method form of speckle imaging used for obtaining high quality images from a number
of short exposures with varying image shifts. Astronomers use this method as well as many image stabilization
functions on cameras.
By calculating differential shifts of the images based on location of items within the image (such as stars), the
images can be aligned and averaged together, creating signal to noise ratio increases and dynamic contrast
ranges. For microscopy, this is best used for fluorescent imaging and many biological microscopy techniques
that require low light environments.
The “Image Stacking” tool is used by recording a short video to get a high quality set of images at ease without
worrying about image rotation, shifting, or scaling between the series of images.
1. Begin by capturing a short video of the sample desired to image stack, or locating a video that the user
would like to use that was pre-recorded. It does not need to be moved in any direction, nor moving. The more
still the video, the better the final stacked product will be.
2. When ready, click the “Image Stacking...” function in the “Process” menu. The software will open a browse
window, asking which video it should use for image stacking. Select the correct one, and hit “OK.” Note that
the software supports .wmv, .asf, .avi, .mp4, .m4v, .3gp, .3g2, .3gp2, .3gpp, .mov, .mkv, .flv, .rm, and .rmvb file
formats for image stacking.
3. The software will scan the video and process multiple frames into alignment, then average them, to provide
the stacked image as the final result once processing is complete.