Fluo-100 Portable Fluorometer Operation Manual
Chapter 5
Software Introduction
16
Fig5.19 View Curve List
5.2.2.2 Detecting
You can use the standard curve to detect the sample. Click “Detecting” to enter the interface
(Fig5.20).
Fig5.20 Detecting Interface
You can set the volume of original sample in the PCR tube, thus the concentration of the
original sample will be calculated after detecting. Click “-” or “+” to set the volume.
NOTE
: Sample volume is 200uL for 0.5ml qPCR tube,100uL for 0.2ml qPCR tube; Range
of sample volume is 1-20uL.
Click “Detecting” to detect after putting test tube into instrument. Fluorescence value will
be displayed after detecting,and concentration will be calculated according to standard
curve.
Sample volume
setting
Original sample
concentration
Sample concentration
in PCR tube
Sample fluorescence
