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Allsheng OZYME Fluo-100 Series, Operation Manual

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Allsheng OZYME Fluo-100 Series Operation Manual Download Page 18

Fluo-100 Portable Fluorometer Operation Manual

Chapter 5

Software Introduction

13

Fig5.13 Home>dsDNA>Standard Curve

1. Parameter

Click Parameter and set excitation or emission wavelength and concentration unit

of curves(Fig5.14).

Fig5.14 Home>dsDNA>Standard Curve>Parameter

2. Curve Fitting

Click “Curve Fitting” and standard samples are detected to fit standard curve. You

can set concentration of standard sample in this interface,then check the fitting curve,

Summary of Contents for OZYME Fluo-100 Series

Page 1: ...Operation Manual V 1 0 Fluo 100 Portable Fluorometer Hangzhou Allsheng Instruments Co Ltd...

Page 2: ...ck the instruments as well as all accessories with packing list when you first open it If you find anything wrong or missing please contact distributor or manufacturer HANGZHOU ALLSHENG INSTRUMENTS CO...

Page 3: ...Indoor use only Read this manual carefully before operating the instrument Only the qualified person who have the skill or experience can operate it Disassemble the instrument without permission is fo...

Page 4: ...g cases and contact supplier or skilled maintenance to manage There is some liquid flowing into the Instrument Drenched or fire burned Instrument dropping or outer shell damaged Malfunction The Instru...

Page 5: ...Installation Steps 4 4 4 Operating Considerations 4 Chapter 5 Software Introduction 6 5 1 Self Testing 6 5 2 Home Interface 6 5 2 1 Fluorescence Detection 7 5 2 2 dsDNA Detection 9 5 3 Report 17 5 4 S...

Page 6: ...s of low concentration such as protein enzyme receptosome antibody nucleic acid hormone steroid thyroid hormones medicine and microorganism Fluo 100 fluorometer is based on fluoroimmunoassay to detect...

Page 7: ...ission wavelength Fluo 100A Flurometer UV LED 365 20nm 420 480nm 60nm Blue LED 460 20nm 525 570nm 45nm Fluo 100B Flurometer Blue LED 460 20nm 525 570nm 45nm Red LED 625 20nm 670 725nm 55nm Fluo 100C F...

Page 8: ...cture This chapter introduces the structure of instrument If it is used for the first time please read this chapter carefully to make a better preparation Structure 1 Fig3 1 Structure1 Structure 2 Fig...

Page 9: ...Check instrument appearance if there is cracks damage or deformation 4 2 Installation Conditions Put on the dry clean and horizontal worktable Working conditions Clean air without corrosion steam or...

Page 10: ...sting Fig4 1 Sample Test Steps To insure the effect of test results volume of sample should be 3ml for cuvette o 1ml for 0 2ml fluorescence PCR tube and 0 2ml for 0 5ml fluorescence PCR tube Please us...

Page 11: ...will start self testing once powered on Fig5 1 Starting Interface 5 2 Home Interface Instrument enters into below home interface after self testing finished Fig5 2 Home Interface There are three func...

Page 12: ...curve building or concentration analysis But you can set and calibrate standard curve detect sample by clicking the other five icons The function of the five icons is same It is convenient for you to...

Page 13: ...enter normal detection mode of fluorescence detection Fig5 4 Fig5 4 Home Fluorescenter Blue 460nm 460nm Kinetic Detection Mode Click the button to enter 460nm kinetic detection interface as below Fig5...

Page 14: ...eck the kinetic curve Fig5 7 Fig5 7 Kinetic Detection Curve NOTE Different channel has different excitation and emission wavelength see the details in Chapter 2 5 2 2 dsDNA Detection NOTE The software...

Page 15: ...sample NOTE If there is no standard curve this item is not available Standard Curve Use standard sample to build standard curve for calculating sample concentration Calibration Curve Calibrate standar...

Page 16: ...ch was exported from Fluo 100 to the U flash disk before Click Im curve to choose the curve data you want to import Fig5 10 Export Curve Export curve which is useless for the moment to U flash disk Cl...

Page 17: ...er 5 Software Introduction 12 Fig5 11 Export Curve Fig5 12 Delete Curve 5 2 2 1 Standard Curve Choose a Curve ID or create a new curve to enter Standard Curve interface Fig5 13 In this interface there...

Page 18: ...Click Parameter and set excitation or emission wavelength and concentration unit of curves Fig5 14 Fig5 14 Home dsDNA Standard Curve Parameter 2 Curve Fitting Click Curve Fitting and standard samples...

Page 19: ...for fitting standard curve and one average value at least Check standard curve Check standard curve when fluorescence values of standard sample are over three Fig 5 16 Fig5 16 Curve Checking Click to...

Page 20: ...ample Check the next sample Delete the current standard sample data 3 View Curve View concentration fluorescence values and equation of current curve Fig5 17 Fig5 17 Home dsDNA Standard Curve View Cur...

Page 21: ...be thus the concentration of the original sample will be calculated after detecting Click or to set the volume NOTE Sample volume is 200uL for 0 5ml qPCR tube 100uL for 0 2ml qPCR tube Range of sample...

Page 22: ...t of samples 1 3 to improve the accuracy of detection Fig 5 21 It also helps to save time and samples of setting standard curve Fig5 21 Calibration Interface NOTE You can prepare 1 3 samples to calibr...

Page 23: ...e different kinds of test data Fig5 23 Fig5 23 Report List View the selected results Delete and print the selective data In the view interface Concentration is the concentration of original sample Vol...

Page 24: ...e Introduction 19 NOTE Once the data is deleted it can not be recovered Fig5 24 View Interface 5 4 Settings You can set Time LCD Print and Update in settings interface Fig 5 25 Fig5 25 Settings Interf...

Page 25: ...ings Time Save the date and time you have setted and back to the Settings interface Give up setting and back to the Settings interface 5 4 2 LCD Click LCD to set the screen Fig5 27 Fig5 27 Settings LC...

Page 26: ...tness Fig5 29 Fig5 29 Settings LCD Brightness 5 4 3 Print This instrument is equipped with a thermal printer via USB to connect with Click Print to enter the setting interface Fig5 30 Select the mode...

Page 27: ...5 Software Introduction 22 Fig5 30 Settings Print 5 4 4 Updata Insert the U disk which has stored new version of software and click OK button to update the instrument Fig5 31 You need to restart the...

Page 28: ...rces Instrument already calibrated before delivery User is not allowed to disassemble the instrument Any deficiency occurs please contact manufacturer Continuous emergency turning on off is not allowe...

Page 29: ...fective Check the power supply Check whether the plug is loose 2 Light source can not light up Power of light source failure Module poor contact Check the power Check whether the module is loose 3 Mea...

Page 30: ...tem Unit Qty Remark 1 Power adapter EA 1 9V 1 5A 2 0 5ml PCR tube holder EA 1 3 User manual EA 1 4 Usb stick EA 1 8 GB 5 Certificate EA 1 Nous contacter Service client commande commande ozyme fr Servi...

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