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Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
3
Using the Fluorescence Detector
Advanced Settings
Figure 24
Peakwidth Settings (G7121B)
Figure 25
Peakwidth Settings (G7121A)
Peakwidth
(Responsetime, Data Rate)
Peakwidth enables you to select the peak width (response
time) for your analysis. The peak width is defined as the
width of a peak, in minutes, at half the peak height. Set the
peak width to the narrowest expected peak in your
chromatogram. The peak width sets the optimum response
time for your FLD. The peak detector ignores any peaks that
are considerably narrower, or wider, than the peak width
setting. The response time is the time between 10 % and
90 % of the output signal in response to an input step
function.
Limits: When you set the peak width (in minutes), the
corresponding response time is set automatically and the
appropriate data rate for signal and spectra acquisition is
selected as shown.
Stoptime/Posttime
The
Stoptime
is the time where either the complete system
stops (As Pump/Injector) or the module (if different from
system stop time). The data collection is stopped at this
time. A
Posttime
period can be used to allow module’s
items to equilibrate (e.g. after gradient change or
temperature change).
PMT Gain
is the photomultiplier gain. Each step
approximately doubles the signal. A typical setting is
between 10 and 15; use lower settings for very high
concentrations. Higher PMT Gain may improve
signal-to-noise. See also
FLD Scaling Range and Operating
Conditions
.
Limits: 0 to 18 in steps of 1. Default value is 10