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Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
5
Optimizing the Detector
Example: Optimization for Multiple Compounds
Example: Optimization for Multiple Compounds
Using PNAs as a sample, this example uses the described scanning functions.
Setting the Chromatographic Conditions
This example uses the following chromatographic conditions (the detector
settings are shown in
Table 14
Chromatographic Conditions
Mobile phases
A = water = 50 %
B = Acetonitrile = 50 %
Column
Vydac-C18-PNA, 250 mm x 2.1 mm i.d. with 5 µm particles
Sample
PAH 0.5 ng
Flow rate
0.4 ml/min
Compressibility A (water)
46
Compressibility B (Acetonitrile)
115
Stroke A and B
auto
Time Table
at 0 min % B=50
at 3 min % B=60
at 14.5 min % B=90
at 22.5 min % B=95
Stop time
26 min
Post time
8 min
Injection volume
1 µl
Oven temperature (1200)
30 °C
FLD PMT Gain
PMT = 15
FLD Response time
4 s
