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Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Calibration Procedure
2
Preparation of the Flow Cell.
a
Flush the flow cell with water.
b
Remove the inlet capillary from the flow cell.
c
Take the syringe and fix the needle to the syringe adapter.
d
Suck about 1.0 ml of the calibration sample into the syringe.
e
Keep the syringe in a horizontal position.
f
Remove the needle.
g
Add the filter to the syringe and fit the needle to filter.
Figure 67
Syringe with Sample Filter
h
Lift the needle tip and carefully eject approximately 0.5 ml to remove air
out of the syringe and to flush the needle.
i
Add the PEEK fitting to the needle tip and fix both at the flow cell inlet.
j
Slowly inject about 0.2 ml and wait for about 10 seconds to inject another
0.1 ml. This will assure that the cell is filled properly.
3
Wavelength Calibration.
a
From the user interface start the FLD wavelength calibration.
•
Agilent Lab Advisor:
Calibrations
•
Instant Pilot (G4208A):
Maintenance > FLD > Calibration
6DPSOHILOWHU
N O T E
Do not inject the calibration sample without the sample filter.
N O T E
If the wavelength calibration process fails, refer to
