Agilent Technologies G4782A, User Manual

Page: 39 / 202

1260 SFC Binary Pump 39
Using the Pump
3
Hints for Successful Use of the Binary Pump
Hints for Successful Use of the Binary Pump
•
Place solvent cabinet with the solvent bottles always on top (or at a higher
level) of the pump.
•
Flush the degasser with at least 5 mL per channel before operating the
pump, especially when the pumping system had been turned off for a
certain length of time (for example, overnight) and volatile solvent mixtures
are used in the channels.
• Prevent blocking of solvent inlet filters (never use the pump without solvent
inlet filters). Growth of algae should be avoided (see
“Prevent Blocking of
Solvent Filters” on page 53).
•
Check purge valve frit and column frit in regular time intervals. A blocked
purge valve frit can be identified by black, yellow or greenish layers on its
surface or by a pressure greater than 10 bar in low delay volume
configuration and 20 bar in standard configuration when pumping distilled
water at a rate of 5 mL/min with an open purge valve.
•
Whenever possible use a minimum flow rate of 5 μL/min per solvent
channel to avoid crossflow of solvent into the unused pump channel.
• Whenever exchanging the pump seals, the purge valve frit should be
exchanged, too.
• Check the pump pistons for scratches, grooves and dents when changing
the piston seals. Damaged pistons cause micro leaks and will decrease the
lifetime of the seals.
•
After changing the piston seals, apply the seal wear-in procedure (see “Seal
Wear-in Procedure Channel B” on page 115).