Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
81
Optimization of the Agilent 1260 Infinity Quaternary LC VL
3
Optimizing Detection with VWD
Set the Detector Parameters (VWD)
1
Set peakwidth as close as possible to the width (at half height) of a narrow
peak of interest.
2
Choose the sample wavelength.
•
at a longer wavelength than the cut-off wavelength of the mobile phase,
•
at a wavelength where the analytes have strong absorptivity if you want
to get the lowest possible detection limit,
•
at a wavelength with moderate absorptivity if you work with high
concentrations, and
•
preferably where the spectrum is flat for better linearity.
3
Consider to use time-programming to further optimization.
N O T E
However you have to be aware that there are additional tolerance of gasket thickness and
its compression ratio which is supposed to be very small in comparison with the machining
tolerance.
Summary of Contents for 1260 Infinity Quaternary LC VL
Page 1: ...Agilent Technologies Agilent 1260 Infinity Quaternary LC VL System Manual and Quick Reference...
Page 6: ...6 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Contents...
Page 36: ...36 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference 1 Introduction Detector...
