ADInstruments ML1001 Owner'S Manual Download Page 3

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This document was, as far as possible, accurate at the time of release. However, changes may have been made

to the software and hardware it describes since then. ADInstruments Pty Ltd. reserves the right to alter

specification as required. Late-breaking information may be supplied separately.

Trademarks of ADInstruments

LabTutor ®, LabChart®, PowerLab® and MacLab® are registered trademarks of ADInstruments Pty Ltd.

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LabAuthor, Chart and Scope (application programs) are trademarks of ADInstruments Pty Ltd.

Other Trademarks

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making science easier

INSTRUMENTS

Summary of Contents for ML1001

Page 1: ...making science easier ADINSTRUMENTS ML1001 Electronic Stimulator Owner s Guide ...

Page 2: ...making science easier ADINSTRUMENTS ML1001 Electronic Stimulator Owner s Guide ...

Page 3: ...ab are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 15T are trademarks of ADInstruments Pty Ltd LabAuthor Chart and Scope application programs are trademarks of ADInstruments Pty Ltd Other Trademarks Apple Mac and Macintosh are registered trademarks of Apple Computer Inc Windows Windows 2000 Windows XP and Windows Vista are either registered...

Page 4: ...t capacity of up to 50 V Directly touching the output terminals or electrodes during stimulation or when the power cord is connected during inspection and the stimulator is operable may result in electric shock Precautions regarding maintenance This alerts the user to imminent death or serious injury arising from misuse of the stimulator This alerts the user to possible death or serious injury ari...

Page 5: ...e instrument is in perfect operating order 2 Check that the instrument is grounded properly 3 Check that all cords are connected properly 4 Pay extra attention when the instrument is combined with other instruments to avoid misdiagnosis or other problems 4 During Operation 1 The instrument must receive continual careful attention 2 Turn power off or remove electrodes and or transducers when necess...

Page 6: ...ii Operator s Manual 1001 CAUTION United States law restricts this device to sale by or on the order of a physician ...

Page 7: ...ence source If this is not possible use a different power supply 3 Effect of direct or indirect electrostatic discharge Make sure all users and patients in contact with the equipment and or system are free from direct or indirect electrostatic energy before using it A humid room can help lessen this problem 4 Electromagnetic interference with any radio wave receiver such as radio or television If ...

Page 8: ...tifact may appear by electromagnetic interference and this may cause mis diagnosis When unexpected artifact appears inspect the surrounding electromagnetic conditions and remove this artifact source If the above suggested remedial actions do not solve the problem consult your distributor for additional suggestions NOTE about Waste Electrical and Electronic Equipment WEEE directive 2002 96 EEC For ...

Page 9: ......

Page 10: ...IX MODU DC ALTN 16 Memory 18 Rear 19 Power 19 External Signal Input Terminals 20 Synchronizing Signal Output Terminal 22 Serial Output 23 General Handling Precautions 24 Preparation 26 Installing the Stimulator 26 Connection of Cables 26 Specifications 28 Stimulation Trigger 28 Main Interval 28 Stimulation Waveform Setup 28 Stimulation Output 29 Synchronized Output Signal 30 Language 30 Power Sour...

Page 11: ...tion ensuring simple reliable operation Field stimulation The built in booster amplifier with a maximum output of 50 V and 400 mA enables direct field stimulation with any stimulation waveform Simple biphasic pulse stimulation Mixing and modulation input terminals that allow output of mixed and modulated waves through combination with an external signal Alternate stimulation by the addition of an ...

Page 12: ...Introduction 3 Configuration Peripheral Devices PowerLab TRIG OUTPUT to ISO Electronic Stimulator 1001 Patch Clamp Amplifier Microelectrode Amplifier Membrane Potential Fixing Amplifier Isolator Fig 1 ...

Page 13: ...tor 1001 Hardware Configuration EEPROM Amplifier TRIG LEVEL Settings Stimulation Output Voltage Stimulation Signal Range Information Stimulation Voltage Detected Value SCI A Serial 1 Communications Unit F ROM for writing Isolator Fig 2 ...

Page 14: ...d by the manufacturer Connection to non specified devices may result in electric shock to the operator by current of leakage H CAUTION Do not touch the connector pins Discharge electrostatic energy by touching a grounded metal part before connecting The instrument may be damaged by electrostatic energy Front Rear STOP INPUT MODU MIX MAIN START MAIN KEY START EXT TRIG EXT TRIG SPEAKER OFF ON OUTPUT...

Page 15: ...cription H Caution See attached materials Serial I O Terminal 8 Power ON 6 Input Terminal 9 Power OFF 7 Output Terminal F ON position Power ON Equipotential Ground Terminal G OFF position Power OFF Positive Polarity 1 Alternative Current AC The products marked with this symbol apply to the European WEEE directive 2002 96 EEG and require separate waste collection For the products labeled with this ...

Page 16: ...n When the power is turned on all the LEDs light once self check Name 3Keys 4Dial Function Press to adjust the set values The indicator for the selected key blinks The set value changes each time the key is pressed Adjusts the set value selected with the selector key Turn clockwise to increase the value and counterclockwise to decrease the value When the dial is pressed the last selected set value...

Page 17: ... indicator blinks It automatically returns to CYCLES after about 10 seconds SET CYCLES can be changed by turning the dial while the indicator is blinking While CYCLES is displayed it is synchronized with stimulation output and shows the stimulation count Setting range 1 to 999 or to continuous operation run When the START signal is received the set number of pulses is output by MAIN INTERVAL at th...

Page 18: ...mes difficult to tell whether MAIN INTERVAL is operating when a long interval has been set operation can be judged by checking the lamp Fig 9 MAIN INTERVAL operation when SET CYCLES is set to 008 Name AMAIN INTERVAL operation lamp Disabled MAIN INTERVAL signal Operating Stopped Operating ...

Page 19: ...s a trigger pulse It is used to control the stimulation repeat cycle by an external signal When EXT is selected with the trigger selector button stimulation occurs in synchronization with the signal that is input to the external trigger terminal on the rear of the stimulator B Fig 10 ...

Page 20: ...y position on the oscilloscope tube by setting the level adjustment knob Allowing for a noise margin and hysteresis in the built in comparator use a trigger signal to oscillate up to about 1 V lower than the trigger level Fig 11 When set to SYNC When the knob is turned fully counterclockwise until it locks SYNC position the trigger level is approximately 3 V When activating the trigger at a pulse ...

Page 21: ...signal for stimulation output The stimulation wave is determined by the four parameters in the figure below Fig 12 T2 DELAY delay time from trigger to start of stimulation T3 DURATION duration of stimulation pulse T4 INTERVAL interval between stimulation pulses n TRAIN number of stimulation pulses C D E F G H Fig 13 Trigger Stimulation wave Delay signal Train end signal Interval signal ...

Page 22: ...ulse duration T3 The timing is shown in Fig 12 Number Setting range Unit 3 digits 10 µsec to 99 9 sec s ms µs Sets the number n of stimulation pulses Fig 12 shows an example when n 5 Setting range 0 to 999 Single pulse stimulation occurs at n 001 pair pulse stimulation at n 002 and train pulse stimulation at n 003 or over Fig 14 When run set value 000 is set continuous stimulation occurs After the...

Page 23: ...he same method as for other settings Normally setting is 0 mV or from 10 mV to 10 V If 50 V is selected setting is 0 mV or from 10 mV to 50 V 1 mV to 9 mV cannot be set This setting does not affect the mixing signal or modulation signal DC voltage in DC mode is also set When negative polarity is selected with the polarity selector key it is read as it is as minus voltage Output precision in relati...

Page 24: ...ignal 1 2 15V 3 15V 4 SIGNAL OUT 5 Range data 6 E 7 Range data synchronizing clock 8 9 10 Void Fig 16 As the output waveform is the same as that of the isolator output connector this is useful for monitoring the stimulation waveform It can also be used for the stimulation signal to the microelectrode amplifier or membrane potential fixing amplifier However when output is switched to 50 V the wavef...

Page 25: ...iphasic waveform The lamp lights Selects a time percentage in relation to the value set by DURATION of the positive polarity pulse in biphasic waveform The set value becomes the time percentage of the positive polarity pulse and the remaining time becomes the negative polarity pulse The lamp for the selected key lights When the MIX key is pressed the set stimulation waveform can be combined with a...

Page 26: ...plitude as the input signal Press this key to create DC stimulation The lamp lights When this key is pressed a DC signal is output regardless of the setting or action of the stimulation waveform setup described above The output voltage is set by VOLTAGE When the polarity selector key is set to plus voltage is generated and when it is set to minus voltage is generated There is no output when set to...

Page 27: ...d for retrieval later Name TMemory keys P1 P2 Function To save the settings press P1 or P2 for more than 2 seconds The current settings are saved to the selected memory Pressing P1 or P2 briefly later will retrieve and apply the saved settings T Fig 20 ...

Page 28: ...r IN connector AC SOURCE Function Connect the ground wires of accessory devices to ground the devices Contains a time lag fuse The fuse should only be changed by Nihon Kohden sales personnel or a qualified electrician To change the fuse turn the groove in the holder with a screwdriver remove the fuse and insert the supplied new fuse Connect the supplied power cable to supply AC power to the stimul...

Page 29: ...the front panel Input the signal to this terminal to stop stimulation by an external signal The function is the same as the STOP button on the front panel The input signal conditions are the same as for START Input a signal to this terminal to trigger stimulation by an external signal Setting range 0 to 10 V See External trigger level knob Input conditions Input impedance approx 100 kilohms Allowa...

Page 30: ...key on the front panel after connecting the signal Input conditions Input impedance approx 100 kilohms Effective input voltage range 10 V Input the signal to this terminal to modulate the external waveform by internal oscillation When the waveform has been modulated by the internally created pulse it can be output by pressing the MODU key on the front panel after connecting the signal Input condit...

Page 31: ...s STOP INPUT MODU MIX MAIN START MAIN KEY START EXT TRIG EXT TRIG SPEAKER OFF ON OUTPUT INTERVAL DELAY MAIN INTERVAL SET CYCLES END TRAIN END 7 6 Name Main interval output terminal MAIN INTERVAL Set cycle end signal output SET CYCLES END External trigger output terminal EXT TRIG Function Generates a pulse that is synchronized with the MAIN INTERVAL trigger signal Generates a pulse that is synchron...

Page 32: ...FF Function Generates a pulse that is synchronized with the delay signal generated by stimulation waveform setup See Fig 12 for the timing Generates a pulse that is synchronized with the interval See Fig 12 for the timing Generates a pulse that is synchronized with the train end signal generated by stimulation waveform setup See Fig 12 for the timing Switches the synchronized sound on and off ...

Page 33: ...et automatically according to the following eqvation Fig 27 Setting is performed in the order T1 T2 T4 T3 n Fig 28 2 Make sure that operation has stopped before pressing the keys If the keys are pressed while the stimulator is operating the new setting will be reflected in stimulator operation immediately Care must therefore be taken as unexpected stimulation may result 3 When all the number setti...

Page 34: ...this may adversely affect the performance of the stimulator 6 When the power is turned on output is momentarily unstable and a spike pulse may be generated When stimulating this spike may become the stimulation pulse Turn the power on before attaching the electrodes 7 Do not use the stimulator with the cover on as this may cause overheating ...

Page 35: ... failure 7 If any irregularity occurs when the power is turned on turn the power off immediately and unplug the power cable from the power outlet At the same time mark the stimulator clearly as Out of Order and do not use it until it has been inspected and repaired Connection of Cables 1 Ground wire Connect the supplied ground wire to the ground terminal and connect the other end to a reliable gro...

Page 36: ...tor tighten the screw firmly to prevent the cable from coming loose When connecting the stimulation output cable connect the cable securely to prevent it from coming loose 4 If necessary connect an oscilloscope and recorder and data acquisition analysis system etc as peripheral devices to measure and record phenomena after stimulation ...

Page 37: ...ms and over 3 SET CYCLES Setting range 1 ms to 9990 s within 0 1 Unit ms s Number 3 digits 4 CYCLES Repeat count 1 to 999 Continuous stimulation when set to run 5 External trigger TRIG LEVEL 0 to 10 V continuous variable Approx 3 V when turned fully counterclockwise Input impedance 100 kilohms or over Stimulation Waveform Setup 1 DELAY Setting range 0 to 99 9 s 1 within 2 µs Unit µs ms s Number 3 ...

Page 38: ...G Mixing method Analog addition Mixing voltage range 0 to 10 V Voltage after mixing max 10 V Linearity within 7 at 1 V to 10 V Input impedance 100 kilohms or over 4 DC Output voltage Direct current 0 to 10 V Direct current 0 to 50 V when 50 V output is selected 5 Isolator output to ISO Pin 1 2 15 V 3 15 V 4 Signal output 5 Range data 6 E 7 Range data synchronizing clock 8 Void 9 Void 10 Void The f...

Page 39: ...TRIG DELAY INTERVAL TRAIN END 2 Output signal Amplitude 5 V Pulse width approx 500 µs Rising 3 µs or less Output impedance 1 kilohm or less Language English Power Source Voltage AC 110 V 220 V or 240 V 10 Changed voltage range by the connector Frequency 50 60 Hz Power input 70 VA Environment Main operating environment Laboratory Operating Environment Temperature 10 to 40 C Humidity 30 to 90 Atmosp...

Page 40: ...ee of protection against harmful ingress or liquids IPX0 devices w o special protection Mode of operation Continuous operation Voltage endurance AC 1500 V 1 minute Leakage current 100 µA or less for both earth leakage and enclosure leakage Insulation resistance 50 megohms or over DC 500 V Mechanical strength of stimulator Compliant with impact NS7231 5 7 vibration NS7231 5 8 Dimensions and Weight ...

Page 41: ...000 4 2 Floors should be wood concrete or ceramic tile If floors are covered with synthetic material the relative humidity should be at least 30 Mains power quality should be that of a typical commercial or hospital environment Mains power quality should be that of a typical commercial or hospital environment Power frequency magnetic fields should be at levels characteristic of a typical location ...

Page 42: ...dless telephones and land mobile radios amateur radio AM and FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy To assess the electromagnetic environment due to fixed RF transmitters an electromagnetic site survey shoule be considered If the measured field strength in the location in which the 1001 is used exceeds the applicable RF compliance level above the 1001 s...

Page 43: ...tters rated at a maximum output power not listed above the recommended separation distance d in meters m can be estimated using the equation applicable to the frequency of the transmitter where P is the maximum output power rating of the transmitter in watts W according to the transmitter manufactuer NOTE1 At 80 MHz and 800 MHz the separation distance for the higher frequency range applies NOTE2 T...

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