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Switch off procedure 

1)

 

Remove the specimen from the microscope stage 

2)

 

Wipe off excess immersion fluid from the 100x oil objective lens/top 
using the Whatman 105 lens cleaning tissues provided  

The 10x and 20x air objectives don’t need cleaning after use.

 

3)

 

Leave the 10x objective selected on the microscope nose-piece to 
minimise any possibility of damage during start-up when the XY 
motorised stage initialises  

Note: The top of the 100x objective rotates, so avoid accidently turning it. 

4)

 

Drop the 10x objective nearer to the Load (lowest) position using the 
microscope coarse focus control 

5)

 

Transfer image files to the network or data key 

6)

 

Shut Down the PC workstation 

7)

 

Switch off all 13A mains sockets around the microscope, leaving the 
modules switched on  

8)

 

Tidy up the microscope area and leave.  

9)

 

Switch the room light, desk lamp, and red lamps off as you leave the 
room. 

 

Содержание ELYRA

Страница 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Zeiss Elyra TIRF Confocal Abridged INSTRUCTIONS...

Страница 2: ...super resolution with temperature control Plan Apochromat 10x NA 0 3 air objective Plan Apochromat 20x objective NA 0 8 air objective Alpha Plan Apochromat 100x NA 1 46 oil objective Andor iXon DU 89...

Страница 3: ...ain with the camera it s locked in software The EM Gain going from 1 to 200 goes from the equivalent of second to a 20ms to frame rate Avoid putting too much light onto the cameras EMCCD sensor The EM...

Страница 4: ...r use If you are using the heated incubator at 37oC you should switch on the incubator at least 2 hours before imaging For the most stable time lapse imaging at 37oC leave the incubator on overnight T...

Страница 5: ...yra microscope The tube lens can be 1x or 1 6x always use the 1 6x with super resolution If the 1x tube lens is selected you can see the shadow of the 1 6x down the microscope vanishes when the 1 6x i...

Страница 6: ...n OOO appears on the display this is the lamp intensity Press Up Down to select 0 12 25 50 and 100 lamp intensity Warning things can very bright down the eyepieces even with the standard FITC TRITC Fa...

Страница 7: ...ails on the operation of the Elrya TIRF super resolution microscope see the Ziess Elyra manual and in particular the Elyra Quick Guide after chapter 7 of the manual a pdf download of the Quick Guide i...

Страница 8: ...ith excitation light in combination with open close filter options in Zen Black 8 Ensure that the doors and viewing panels are closed on the black stage incubator or the lasers will switch off Wait 5...

Страница 9: ...y possibility of damage during start up when the XY motorised stage initialises Note The top of the 100x objective rotates so avoid accidently turning it 4 Drop the 10x objective nearer to the Load lo...

Страница 10: ...ave any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG...

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