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The X-cite Series 120PC Q
The X-Cite takes a minute or so warm up
Use the Mode button to select three modes:
1.
Press mode and when OOOO. appears on the display this is the number
of hours the arc lamp has been on
2.
Press mode and when OOO.O appears on the display this is the timer
mode. The display is in seconds and 1/10 seconds. It times how long the
lamp will be on in seconds. Set the time and press Start (Stop) and lamp
will come on and the timer will count down to switch-off.
3.
Press mode and when OOO appears on the display this is the lamp
intensity. Press Up/Down to select 0, 12, 25, 50 and 100% lamp
intensity. Warning things can very bright down the eyepieces even with
the standard FITC/TRITC/Far red triple filter selected.
Press Start/Stop to switch open/close the X-Cite shutter and illuminate the
specimen. The footswitch under the microscope also activates the Start/Stop
sequence.
Note that the Excite 120PC Q only works with the triple filter block FSet77HE
(for GFP/DsRed/Cy5 only and so no DAPI) when viewing down the eyepieces.
The triple filter FSet77HE excites at 483 + 564 + 642 nm (HE) and shows all
three fluorescence colours at the same time, although our eyes can’t see the
far red Cy5 emission.