RCF Microscopy Manual
5/2/2017
Authors Cory Nook and Chaowei Shang
Page 8 of 19
Obj
Objective
Medium Grid
10x EC Plan-Neofluar 10x/0.3 DIC I
Air
L
20x Plan-Apochromat 20x/0.8 DIC II
Air
L
40x LD Plan-Neofluar 40x/0.6 Ph2 DIC II
Air
NA*
40x Plan-Apochromat 40x/1.3 DIC (UV) VIR-IR
Oil
L
63x Plan-Apochromat 63x/1.4 DIC III
Oil
H
100x Plan-Apochromat 100x/1.4
Oil
D
The 4 buttons on the template move the support brackets along the track. The template will hold a variety of
shapes. If you need a multi-well plate template, please ask staff.
Start with DAPI to find and focus on your sample. Be aware that fluorescence light is damaging to the eyes.
Avoid looking directly at the lasers. Block the light infront of you if the orange light shield is not strong enough
to block the light.
4. START UP THE AxioVision SOFTWARE
Double-click on the AxioVision icon on the desktop to
start the program.
Make sure your Apotome power supply (labeled with
number 4) is turned on before starting up the
software, otherwise apotome may not work properly.
