RCF Microscopy Manual
5/2/2017
Authors Cory Nook and Chaowei Shang
Page 14 of 19
Click off pseudo colors and right click on the images, a menu containing property will appear.
Adjustment of images can be done under the property settings.
5.2 Save and Export
YOU MUST SAVE ALL IMAGES ON THE E: DRIVE IN A FOLDER
WITH YOUR NAME. DO NOT SAVE ANYTHING ON THE C:
DRIVE. THESE IMAGE FILES WILL BE PERIODICALLY REMOVED
BY STAFF, SO YOU MUST BACK UP YOUR FILES ELSEWHERE.
To save an image, click on the experiment
heading and select File in the toolbar. The
format for the proprietary software is .zvi.
Make sure you save all your images in this
format (when publishing data, many
journals require the original format of
images). You may also export in other
formats but it is recommended to export as
a tiff, later. It is also advised that you save
at regular intervals to protect your images
from an unexpected software crash.
To export files, go to File in the toolbar and
click on Export. An export window will
appear with the base name of the desired
file in the window heading, shown
adjacently. Create a name for the file in the
Base name: section. This will give the initial
name for the files to be exported and the
name of the folder to where the files will
go. The Save in: section gives the file path
where your files will be saved. You need to
make sure that these files are going to the
appropriate folder. The output files at the
bottom of the window tell you how many
images will be produced in the desired
format. If you want a merged image of all
the channels then you will need to select
Generate Merged Image(s) below the
channel selection.
TIFF
