RCF Microscopy Manual
5/2/2017
Authors Cory Nook and Chaowei Shang
Page 13 of 19
button is recommended to not only get the
correct camera settings but can be used to
make sure your sample is in focus. This is
where the user needs to decide which part of
the sample is the most important. That part of
the sample needs to be in focused because not
all channels may necessarily be focused at the
same position. When the measure icon is
clicked a new window will pop up as shown.
Displayed is that current channel in black and
white to show over exposed pixels.
Any pixel in
red is over exposed
and cannot be used in
quantification data. Also, over exposure for
long periods of time can damage the camera.
When you have adjusted the exposure time
and focused on your image, click on the OK
button.
Do NOT close the window via the close
icon in the top right of the window.
This can
lead to a “run-time” error and can bleach your
sample if not careful. See the appendix on
“Run-Time Errors” to know how to prevent
sample bleaching.
5. ACQUIRING AND SAVING AN EXPERIMENT
5.1 Acquire
Confirm that you have switched from the eyes mode to the camera mode
After you have completed all of your settings and are ready to capture an image, click Start at the
bottom of the Multidimensional Acquisition Window.
