Image Acquisition with the AxioCam MRc5
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The disadvantage of this technique, however, is that noise is greatly
intensified, and the potential dynamic range of the corresponding image data
is reduced. You should therefore carefully weigh up the benefits of using
digital signal gain before actually employing the technique. For this reason the
recommended value is gain by a factor of 1.
From a technical viewpoint, this technique involves multiplying the data by a
corresponding factor. The maximum numerical pixel value generated is always
limited to the maximum value of the analog/digital converter you are using
(12 bits per pixel per color channel in the case of the AxioCam MRc5).
NOTE:
You can also achieve a bright image display with short exposure times by
making the display characteristic curve steeper.
Image Orientation
An AxioCam MRc5 image is adjusted in such a way that the image orientation
corresponds to the image in the eyepiece when mounted on an upright
microscope with the front of the camera facing you.
This starting position can be adapted via this menu to suit your requirements.
If, for example, the camera’s cable routing needs to be changed on an inverse
microscope, the camera can be mounted at a different angle, without the
correspondence between the image orientation and the eyepiece being lost.
Black Reference
Setting a black reference ensures that the image background retains a
uniform darkness, even with exposure times in excess of several seconds. This
setting is therefore particularly recommended for the acquisition of weak
fluorescence or other images with weak light intensity and consequently
long
exposure times (starting from approx. 3 seconds).
NOTE:
Perform this black-value calibration approx. 15 minutes after switching on
the camera, as the camera will then be in a thermally balanced condition.
