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4-10

(

This is an indirect reading of starch by measuring dextrose liberated from the hydrolysis of
starch. To use the method described, your sample must be dextrose-free. If free dextrose is
present, see Special Considerations below.

First, dissolved starch is hydrolyzed externally by the enzyme Amyloglucosidase to produce
dextrose. Dextrose is read at the enzyme sensor. The enzyme Glucose Oxidase is immobilized
in the YSI Dextrose Membrane.

H

2

O

Amyloglucosidase

β

-D-Glucose (dextrose)

De O

2

Glu Oxidase

H

2

O

2

+ D-Glucono-

δ

-Lactone

System Buffer

YSI 2357

Calibrator Std

YSI 2776 (2.50 g/L dextrose)

Linearity Std

YSI 1531 (9.00 g/L dextrose)*

Black Probe

YSI 2365 Dextrose Membrane

White Probe

n/a **

Sample Size

25

µ

L

Cal Station

Station #1 Call Well

Black Probe Parameters
Chemistry

Dextrose

Unit of Conc

g/L

Cal value

2.50 g/L

End Point

30 sec

* For applications requiring linearity performance to 25.0 g/L, YSI 2777 (25.0 g/L dextrose)
may be used as a linearity standard provided the sample size is 10

µ

L.

** If you have a dual channel unit, either Black or White, or both Black and White probes can
be configured for this chemistry.

Special Considerations:

»

An amyloglucosidase enzyme such as Sigma # A7420 works with many samples.

»

Solubilizing starch is key to obtaining reproducible results. A variety of methods are in
the literature. YSI has documented a couple of methods in YSI Application notes.

»

Prepare a starch solution using the diluent described below, add 1 to 2 mg
amyloglucosidase per mL of starch solution. Allow at least 20 minutes for the enzyme
to react, and the liberated dextrose to reach mutarotational equilibrium, before
measuring the sample. If your reading is higher than expected, you may need to wait
slightly longer, or re-evaluate the possibility of free-dextrose in the sample.

Diluent: 40 g/L NaH

2

PO

4

, 10 g/L Na

2

HPO

4

, in reagent water.

»

If free-dextrose is a concern, first measure free dextrose. Then externally hydrolyze the
starch, and measure the total dextrose after hydrolysis. Subtract the free-dextrose from
the total dextrose and multiply by 0.9 to account for water of hydrolysis.

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Содержание 2700D

Страница 1: ...excess underutilized and idle equipment along with credit for buybacks and trade ins Custom engineering so your equipment works exactly as you specify Critical and expedited services Leasing Rentals D...

Страница 2: ...Biochemistry Analyzer Who s Minding the Planet find out at Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 3: ...aseline Check 2 25 3 1 Main Menu 3 1 3 2 Run Mode 3 2 3 3 Standby Mode 3 7 3 4 Daily Operational Checks 3 7 4 1 Principle 4 1 4 2 Sample Preparation 4 2 4 3 Measurement Parameter Setup Information 4 3...

Страница 4: ...enance 7 1 7 2 Calibration Pumping System Maintenance 7 3 7 3 Sample Chamber Cleaning 7 4 7 4 Membrane Replacement 7 5 7 5 Probe Cleaning 7 6 7 6 Tubing Replacement 7 7 7 7 Sipper Replacement 7 9 7 8...

Страница 5: ...iii A 1 B 1 C 1 D 1 0 1 E 1 2 F 1 3 4 5 2 G 1 6 7 H 1 I 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 6: ...esponse 6 4 Figure 6 4 Aging Membrane Response 6 5 Figure 6 5 2700 SELECT Software Structure 6 7 Figure 7 1 Sample Chamber Illustration 7 5 Figure 7 2 Tubing Connections 7 7 Figure 7 3 Pump Tubing ins...

Страница 7: ...ic gravity temperature index of refraction optical activity or the presence of proteins or other biochemicals The Model 2700 SELECT can be set up to measure a number of different analytes but switchin...

Страница 8: ...ents to support the most common chemistries YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials The 2710 Turntable is controlled by the 2700 SELECT software via a control...

Страница 9: ...imizes carry over Fluid design washes inside and outside of sipper tube Programmable automatic calibration Selectable concentration units including g L mg L mmol L w v Liquid crystal display and built...

Страница 10: ...matrix Power requirement 110 120 VAC or 220 240 VAC 50 60 Hz 50 Watts nominal Working environment Ambient temperature 15 to 35 Celsius Relative humidity 10 to 90 non condensing Instrument dimensions 1...

Страница 11: ...g Color Green Potential Substrate Interference Methanol L Glutamate YSI Membrane 2754 Detection Range 0 10 mmol L Calibration Point 5 00 mmol L Linearity Check Point 10 0 mmol L Precision CV n 10 2 Li...

Страница 12: ...g color Blue Choline YSI Membrane 2771 Detection Range 0 450 mg L Calibration Point 175 mg L Linearity Check Point 450 mg L Precision CV n 10 2 Linearity 0 to Cal Point 2 Linearity Cal to Range Max 5...

Страница 13: ...ends that you confirm that no significant levels of interfering substrates are present in your samples For example we know that lactose and galactose in the same sample may interfere if significant co...

Страница 14: ...ust a matter of knowing which buttons to push An understanding of the principles of operation and potential chemical interferences is necessary for the wisest interpretation of results Thorough precau...

Страница 15: ...nect the plug to a matching three pronged wall receptacle 3 Use ONLY fuses of the type supplied Section 7 9 Replacement power cords and fuses can be obtained from YSI or your Dealer Representative App...

Страница 16: ...urself with the major components NOTE In maintenance kits service manuals and part lists the Sipper may be referred to as the Sipper Needle or Sipper Tube Figure 2 1 Inside Front View of the 2700 SELE...

Страница 17: ...tles are empty or when the Waste bottle is full The Sample Chamber is made of clear acrylic plastic White and black holders for the sensor probes are screwed to either side The immobilized enzyme memb...

Страница 18: ...se for fuse replacement The Power Receptacle is a power inlet One end of the power cord plugs into this receptacle while the other end plugs into an electrical outlet The Power Switch is an on off tog...

Страница 19: ...ce the lid and reconnect the lead Install the calibrator solution One or more of the following YSI calibration standards will be provided in your starter supplies YSI 2776 Dextrose and L Lactate YSI 2...

Страница 20: ...nel unit For the single channel configuration the white probe is a blank see Figure 2 4 Figure 2 4 Sample Chamber Sensor Configurations One or more packs of YSI Immobilized Enzyme Membranes is provide...

Страница 21: ...ring membrane assembly gently onto the probe face Figure 2 5 Wipe excess salt solution from the probe body then return the probe to the sample chamber Finger tighten the probe retainer so that the O...

Страница 22: ...he instrument will normally arrive with parameters including date and time set from the factory However if the backup rechargeable batteries have run down you will see the Default Parameters message T...

Страница 23: ...Sipper prime the fluid pumps and adjust the stirring Since the adjustments were made prior to shipment this procedure will likely be just a check IMPORTANT The front door must remain open to carry ou...

Страница 24: ...r Test Sipper position The Sipper will descend into the Sample Chamber The Sipper should not contact the stainless steel cone If the Sipper position is still not exactly right press 1 and readjust it...

Страница 25: ...rmal speed at which the stir bar rotates smoothly in the chamber and accelerated speed at which the stir bar loses synchronization with the motor housed below and jumps This jumping action helps clear...

Страница 26: ...rmal operation you will seldom need to enter the Menu Mode but it is important to familiarize yourself with the menu locations of the various parameters over which you have control See Section 5 3 for...

Страница 27: ...ou select parameters related to sampling protocol set parameters that control automatic switching to standby mode and select parameters that will trigger auto calibrations In Sample Protocol you can s...

Страница 28: ...ware to serve your needs for all standard applications Using this command also requires that you reset date time and all other general parameters Press ENTER to confirm No and return to Select Setup m...

Страница 29: ...YSI 2776 Standard 2 50 g L dextrose and 0 50 g L L lactate has been installed into the Calibrator Bottle position The instrument has been powered and the printer paper installed Let us also assume tha...

Страница 30: ...s required Hour 17 Enter hour Enter date and time as required Minute 22 Enter minute Select date format MM DD YY 1 MM DD YY 2 DD MM YY Select and or confirm the format you desire for printed dates mon...

Страница 31: ...e General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 5 for Serial Enter instrument serial number 98 12345 The serial of your instrument is recorded on the...

Страница 32: ...number outside of this range the display will show you the limits Press ENTER to confirm 25 L and return to the Measurement parameter setup menu level Measurement parameter setup 1 SampleSize 2 CalMet...

Страница 33: ...ues and you choose a concentration unit other than g L the value automatically changes That is 0 50 g L becomes 5 62 mmol L or 500 mg L When non standard calibration values are used however the approp...

Страница 34: ...arameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 0 to return to the Select setup level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 3 for RunMode...

Страница 35: ...ple setup requests that we use sample identification Press 2 for Yes Prompt sample ID Yes 1 No 2 Yes Press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight...

Страница 36: ...l shift 5 Sample error Each parameter in this menu level can be used to alter the conditions which trigger autocalibrations For now simply explore each menu parameter Access each parameter by pressing...

Страница 37: ...shows the Select setup menu Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 6 for Default Reset all system parameters No 1 No 2 Yes This menu allows you to reset...

Страница 38: ...istry application Next use this information to setup the 2700 SELECT as we have demonstrated above HINT Use the Print Setup option to confirm that you have correctly setup your instrument Remember Prn...

Страница 39: ...r Diagnostic Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe B LAC 4 23 nA W DEX 3 10 nA 1 Flush 2 Calibrator 3 Sample Observe the probe current values If they are above 6 nA...

Страница 40: ...eration during the first day since autocalibrations compensate for probe current drift When enzyme membrane replacements are required in the future stabilization will occur much more rapidly usually w...

Страница 41: ...de where reagents are conserved In Section 5 you will learn how to change this 2 hour threshold value to better fit your application If you press STANDBY from Main Menu the instrument flushes buffer t...

Страница 42: ...ore calibration cycles will be run automatically The Sipper moves out of the Sample Chamber and enters the Calibrator Well or other preset calibrator station Calibration standard is aspirated into the...

Страница 43: ...diameter and 50 to 100 millimeters long Any container other than a tube with the dimensions indicated should be presented at the Manual Station Station 3 Some double wall plastic tubes may prevent the...

Страница 44: ...3 4 Figure 3 2 Sampling Stations Figure 3 3 The Test Tube Holder Pivoted Out Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 45: ...trument by pressing CALIBRATE After recalibrating repeat the analysis as directed above If your reading is still outside this range proceed for now Later in this section specific actions for nonlinear...

Страница 46: ...above Again compare the value reported by the instrument with the value on the label of the YSI 1531 Standard 9 00 g L dextrose Repeat calibration and sampling if the result is not within specified to...

Страница 47: ...ANDBY Mode Press ENTER then MENU to return to the Main Menu To ensure that your 2700 SELECT is operating properly perform the following operational checks on a daily basis It is advisable to maintain...

Страница 48: ...he chemistry or chemistries you have chosen See the list below to be certain you are testing with the correct standard solution Place your instrument in RUN Mode When the unit is ready the following d...

Страница 49: ...nits other than g L for the linearity test refer to Appendix A for conversion values NOTE If any reading is outside of the specified tolerance limits recalibrate and repeat the linearity test If the r...

Страница 50: ...3 10 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 51: ...s technology allows preservation of the enzyme by immobilizing it in a patented membrane structure The enzyme membrane is coupled with an electrochemical probe and housed in a sample chamber where sam...

Страница 52: ...ences include phenols hydrazine hydrogen sulfide and mercaptans A variety of sample types can be analyzed with the 2700 SELECT Generally the only sample preparation that may be required is dilution of...

Страница 53: ...ctate analyses The parameters you see in these printouts are default values For many applications the default settings will be appropriate for you Now enter the Measurement Parameter menu press 2 for...

Страница 54: ...measure the dextrose reading at the sucrose membrane and automatically subtract it Black Probe Parameter 3 Black In this menu you choose parameters that correspond to the chemistry of interest at the...

Страница 55: ...xtrose may be used as a linearity standard provided the sample size is 10 L If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Sp...

Страница 56: ...tate Unit of Conc g L Cal value 0 50 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if c...

Страница 57: ...a Sample Size 25 L Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Sucrose Unit of Conc g L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or bo...

Страница 58: ...ve a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations...

Страница 59: ...you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with an ethanol concentration close to your sample con...

Страница 60: ...is 10 L If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Special Considerations An amyloglucosidase enzyme such as Sigma A7420...

Страница 61: ...nter an adjusted calibration value when configuring your instrument measurement parameters For example to express results as a choline hydroxide value program the calibrator value as 204 mg L when usi...

Страница 62: ...tandard solutions If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is requ...

Страница 63: ...unit conversion is required Special Considerations Since hydrogen peroxide is typically used to calibrate the YSI Blank Membrane the enzyme catalase is a concern Catalase destroys hydrogen peroxide Ca...

Страница 64: ...o measure MSG monosodium glutamate monohydrate and express the results as mmol L of MSG the calibration parameters listed above are all appropriate If you want to express results as a w v measurement...

Страница 65: ...l Station Station 2 Black Probe Parameters Chemistry Glutamine Unit of Conc mmol L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes...

Страница 66: ...ou are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with a methanol concentration close to your sample conc...

Страница 67: ...ystem Buffer YSI 2357 Calibrator Std YSI 2776 2 50 g L dex 0 50 g L L lact Linearity Std YSI 1531 9 00 g L dex and YSI 1530 2 67 g L L lact Black Probe YSI 2329 L Lactate Membrane White Probe YSI 2365...

Страница 68: ...L Cal Station Two Station 1 dex 2 suc Black Probe Parameters White Probe Parameters Chemistry Sucrose Chemistry Dextrose Unit of Conc g L Unit of Conc g L Cal value 5 00 Cal value 2 50 End Point 30 s...

Страница 69: ...rose reading An alternative method involves using two different and appropriate dilutions of the sample to first measure free dextrose then treat one sample with invertase and analyze for dextrose lib...

Страница 70: ...2357 Calibrator Std YSI 2755 and YSI 2736 Linearity Std YSI 2756 and YSI 2737 Black Membrane YSI 2735 White Membrane YSI 2754 Sample Size 20 L Cal Station Two Station 1 glutamate 2 glutamine Black Pr...

Страница 71: ...tion as required In MENU Mode there are three submenu levels Service Setup and Diagnostics Notice that you may move from RUN Mode or STANDBY Mode to limited menus in Setup However to fully access MENU...

Страница 72: ...can press MENU to get back to the Main Menu and 0 to back up to the previous display However it may be necessary to confirm a response by pressing ENTER before continuing to use the MENU or 0 keys Ple...

Страница 73: ...rocedure was used during initial instrument setup Refer to Section 2 9 for more information The Sipper Arm Assembly will move to the home position Loosen the adjustment screw that holds the Sipper fla...

Страница 74: ...ple chamber of air bubbles during a flush cycle Adjust until stir bar jumps 1 Increase speed 2 Decrease speed Using the 1 Increase and 2 Decrease choices adjust the Stir Bar Lower the speed to observe...

Страница 75: ...ou may find it helpful to use the Menu Flow Chart Figure 5 2 to follow the menu levels Main Menu display Please select instrument mode RUN STANDBY MENU From Main Menu press MENU to display instrument...

Страница 76: ...level Adjust the display contrast 1 Raise contrast 2 Lower contrast General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 3 for RS232 Use this menu to set communication pa...

Страница 77: ...Configuration you will see the following Select configuration Non multidrop 1 Non multidrop 2 Multidrop The configuration option allows operation in either a point to point environment or in a multidr...

Страница 78: ...34AB can be entered as 98 01234 This number is normally set prior to delivery and preserved in memory by battery backup Press ENTER to confirm and return to General setup level Enter instrument serial...

Страница 79: ...ess 2 for CalMethod The default setting is One station The Two Station option is required for some dual channel analyses for example simultaneous dextrose and sucrose determination Select calibration...

Страница 80: ...cursor For example if you had 13 89 mmol L Dextrose entered and you wanted to change to 1 80 g L the calibration value would read 02 50 with the cursor under the 0 You would then need to enter 1 80 a...

Страница 81: ...per descends when sampling at the Manual Station 3 For example with long test tubes and the fluid level near the bottom of the test tube use Low setting Select manual sipper height Medium 1 Low 2 Medi...

Страница 82: ...ample will be aspirated The default location is Station 2 the Test Tube Holder station You may also elect to sample from Station 3 the Manual Station or even Station 1 the Cal well This last choice wo...

Страница 83: ...errors are detected eg baseline instability Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error You may alter any of these parameters to suit your application however you may compr...

Страница 84: ...e 2 Time 3 Sample 4 Cal shift 5 Sample error Press 4 for Cal shift Enter allowable autocal shift in 0 to disable 2 Confirm value shown or enter and confirm any number from 0 to 100 Autocal setup 1 Tem...

Страница 85: ...enzyme membranes You may also elect to choose Detail report for the first few calibrations or samples after a long idle period where no samples or cals are run If you suspect problems with the instru...

Страница 86: ...with the selections and would like to start at the beginning Press ENTER to confirm your choice and return to Select setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Defa...

Страница 87: ...ture sensor in the Sample Chamber the level sensors in the reagent and waste bottles the Sipper capacitance sensing and the optical sensors that control motors the Sipper Pump and sense the Test Tube...

Страница 88: ...1 for Sipper motor to display the following motion selections Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise When you press one of the keys 1 5 a movement of the Sipper sh...

Страница 89: ...bubbles from the chamber wall and probe surfaces You turn the Stir Motor off by pressing 2 again The Stir motor may be activated with the Sipper either in or out of the Sample Chamber Motor diagnosti...

Страница 90: ...diagnostic 1 Home 2 Dispense separator 3 Exercise Press 2 for Dispense separator A short extension of the plunger should occur This simulates dispensing the air bubble into the Sample Chamber after t...

Страница 91: ...otor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe You will observe a brief display message as shown below which then changes to show probe current s You will observe only Black probe current for a...

Страница 92: ...W DEX 112 9 nA 1 Flush 2 Calibrator 3 Sample Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 4 for I O From this menu level you can ru...

Страница 93: ...crystal display The total test takes about 40 seconds Terminate the test at any time by pressing any key The first few characters are shown below I O diagnostic 1 Keypad 2 Display 3 Printer Press 3 fo...

Страница 94: ...You will observe the current status of each of the four level sensors shown below The Sipper will show fluid or air The Cal and Buffer will show OK or Empty The Waste will show OK or Full Level senso...

Страница 95: ...ions Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 0 to return to Select instrument function Select instrument function 1 Service 2 Setup 3 Diagnostic Press 0 to return to Main Menu di...

Страница 96: ...5 26 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 97: ...ter in the YSI 27 Industrial Analyzer and the YSI 23L Blood Lactate Analyzer Most recently the same technology has been employed in the YSI 2300 STAT PLUS Glucose and L Lactate Analyzer and the YSI 15...

Страница 98: ...herefore to the concentration of the substrate REACTION 1 glucose D glucose O2 GOx Glucono lactone H2O2 REACTION 2 H2O2 Pt anode 2H O2 2e The platinum electrode is held at an anodic potential and is c...

Страница 99: ...ELECT fluid and electromechanics system is schematically described below in Figure 6 2 Figure 6 2 2700 SELECT Fluid System Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www...

Страница 100: ...maximum net probe current is about 500 nanoamps The 2700 SELECT monitors the probe baseline current for nA activity and stability If an unstable baseline is detected the buffer pump will continue to...

Страница 101: ...s net calibration currents occurs the instrument repeats calibration The sensors net current for a calibration is displayed and printed An unstable calibration is displayed and printed as a cal shift...

Страница 102: ...level sensor detects the sample surface at the Test Tube Sample station and then travels into the sample about 3 millimeters 1 8 inch This controlled immersion depth permits the use of sample tubes th...

Страница 103: ...DBY You may switch back and forth from RUN Mode to STANDBY Mode by using these keys and confirming your choice by pressing ENTER To return to the Main Menu to have access to the full Menu options of S...

Страница 104: ...6 8 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 105: ...blems related to the enzyme sensor system are indicated Empty the Waste Bottle If the 2700 SELECT is used for medical research or biological testing dispose of the waste bottle contents in a manner su...

Страница 106: ...le with cap You should store it at room temperature After a buffer change prime the buffer system From Main Menu press MENU See menu information displayed above Next press 1 for Service then press 2 f...

Страница 107: ...cial bleach with 3 parts deionized or distilled water and place this solution in a clean 250 mL bottle that has threads compatible with the Cal Cap Assembly at Station 1 Attach the Cal Cap Assembly ti...

Страница 108: ...lockwise 5 Counterclock 6 Exercise Unscrew and remove all three probes from the sample chamber If your membranes are in good condition that is if they pass the tests in Section 3 3 it is not necessary...

Страница 109: ...ction 2 6 to check the Sipper alignment and prime the buffer and calibration systems Figure 7 1 Sample Chamber Illustration To assure proper performance and prevent unexpected down time check membrane...

Страница 110: ...Immediately after soaking rinse the sensor with reagent grade water for 3 5 minutes It is important that all the residual ammonia is removed 3 Prepare a small amount of 0 5 sodium hypochlorite solutio...

Страница 111: ...ment wall and remove the pumps Remove the front housing from each pump by pulling it out at the bottom Remove the roller assembly and tubing from each pump Disconnect and remove all the instrument tub...

Страница 112: ...at the top of the front pump housing see Figure 7 4 Lower the front pump housing while guiding the tubing into the slots at the bottom Gently squeeze the two housings together while pushing up on the...

Страница 113: ...per Refer to Section 2 6 to readjust the Sipper The Sipper Ground Cable flexes with every sample After several thousand samples the cable may break To prevent unexpected down time replace this cable e...

Страница 114: ...3 Fast acting 3AG 250 volt 0 25D x 1 25L inches For Europe and UK use fuse type 220 240 VAC Operation 1 2 Amp YSI 019722 Anti surge T 250 volt 5mm x 20mm L IEC127 BS4265 Install new fuses in place of...

Страница 115: ...strument wall see Figure 7 5 Figure 7 6 Sipper Pump Head Removal Pull the white pump base from the clear pump housing If salt deposits have formed on the parts wash them off with warm water Replace th...

Страница 116: ...assemble the pump position the plunger as shown in figure 7 7 and install it back on the instrument Figure 7 8 Sipper Pump Plunger Position 0 1 2 3 4 5 6 7 Artisan Technology Group Quality Instrumenta...

Страница 117: ...n clean the screw and guide rods with a lint free towel Apply Lubriplate 105 grease supplied in YSI 2788 Preventive Maintenance Kit to two or three threads of the Acme screw Normal operation will dist...

Страница 118: ...7 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 119: ...d leaks salt build ups air bubbles in the chamber loose connections noisy components etc Review Section 5 4 Menu Diagnostics to learn more about how you can test individual components of the 2700 SELE...

Страница 120: ...rmat information Sample Report Detail Calibration Report Detail Sample Report CALIBRATION REPORT ID 123456789 05 03 B Lactate Unstable B L Lactate 4 82 g L IB current 1 79 nA IB current 2 11 nA PL cur...

Страница 121: ...indicator of the presence of an interfering substance End Point is the time from dispensing the sample into the sample chamber until the instrument reads the probe signal The default value for most c...

Страница 122: ...ics and check probe currents SECTION 2 6 POSSIBLE CAUSE Power disruption ACTION Enter probe diagnostics and check probe currents SECTION 2 6 POSSIBLE CAUSE Failing enzyme membrane ACTION Perform daily...

Страница 123: ...ON 2 3 SYMPTOM ERROR Motor Failure POSSIBLE CAUSE One of the motors is jammed ACTION Enter motor diagnostics and cycle the suspected motor SECTION 5 4 POSSIBLE CAUSE Dirty drive screw on sipper mechan...

Страница 124: ...ROR Unable to Calibrate POSSIBLE CAUSE Pinched leaking or disconnected tube ACTION Fix or replace tubing SECTION 7 6 POSSIBLE CAUSE Sipper misaligned ACTION Check Sipper alignment SECTION 2 6 POSSIBLE...

Страница 125: ...disconnected tube ACTION Fix or install new tubing SECTION 7 6 POSSIBLE CAUSE Calibrator pump not operating properly ACTION Replace tubing SECTION 7 6 POSSIBLE CAUSE Fluid not conductive ACTION Use sa...

Страница 126: ...YMPTOM Keypad Disabled POSSIBLE CAUSE Instrument in Remote Control Mode ACTION Switch communications mode or reset SECTION 9 3 POSSIBLE CAUSE Faulty keypad or cable ACTION Check keypad in I O diagnost...

Страница 127: ...n multidrop point to point refers to a single 2700 connected to a host controller The non multidrop configurations of software versions higher than 2 40 maintain compatibility with the earlier softwar...

Страница 128: ...asserted DSR is not used Software Handshaking Signals In the XON XOFF handshaking protocol RTS and DTR are always asserted and CTS and DCD are always ignored Since the 2700 acts as a DTE it requires...

Страница 129: ...mmand A command is defined to be a string of printable upper case ASCII characters Blanks are ignored Command Grammar The command grammar is shown below Abbreviations include ESC escape cmd command ad...

Страница 130: ...t of station is out of range s 1 or s 5 Code 7 purge time of zero is assigned when Station 5 s 5 is assigned monitor accessory Code 8 zero turntable position number is assigned t n 0 or default t n 0...

Страница 131: ...sults by either sending the sample ID number and getting the result record or by querying the unit for any untransmitted result in the data base Remote Control Mode Remote Control Mode is designed pri...

Страница 132: ...e next line If two lines of information are combined the first is black probe data the second is white probe data In single channel units this will not be a concern In Figure 9 4 several examples show...

Страница 133: ...5 12 04 02 13 98 23 56 123 1 H2O2 45 78 nA 0000 cr lf 15 12 04 02 13 98 23 56 123 1 H2O2 15 28 nA 0F01 cr lf 12 02 34 02 13 98 24 86 2 H2O2 12345 78 mmol L 0000 cr lf 12 02 34 02 13 98 24 86 2 H2O2 34...

Страница 134: ...11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 cr lf 11 11 11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 cr lf FIELD OFFSET WIDTH DESCRIPTIONS NO COLUMN BYTE 01 01 08 Time...

Страница 135: ...r RY Report status of instrument RX Report last transmission DATABASE MANAGEMENT RZ Clear database memory CONTROL TR0 Turn remote control off TR1 Turn remote control on TP0 Turn 2700 printer off TP1 T...

Страница 136: ...not sent to host N No unsent calibration results Machine status I Idle in Run Mode S Processing sample C Processing calibration A Processing autocalibration M Processing manual sample P Processing pre...

Страница 137: ...o Host cal result see format in Figure 9 4 When Report Sample Result RS is invoked the 2700 sends back the last sample processed When combined with an ID number the 2700 searches its database and send...

Страница 138: ...N and waiting for successful calibration Then invoke the TR1 command to enter remote control mode In a similar manner you can invoke the Turn Remote Control Off TR0 command to return to Result Reporti...

Страница 139: ...e calibration commands and sample process commands are outlined below The process sample commands are described together the only difference among the different commands being the station from which t...

Страница 140: ...om which to sample If s is not given or NULL the currently assigned sample station is used The character t is the turntable starting position which is only valid when s 4 Remember station 4 is the tur...

Страница 141: ...BB BB CCCCC DDD EEEEEEEEE FFFF GGGGG GG HHHHHHHH II J cr lf AA AA AA BB BB BB CCCCC DDD EEEEEEEEE KKKK LLLLL LL MMMMMMMM NN O cr lf In the fields above the characters represent the following A current...

Страница 142: ...yy 1 for dd mm yy c bottle level sensor flag 0 for off 1 for on d sample size 5 to 65 L e sample station 1 to 5 f calibration method 0 for single 1 for dual Autocal g autocal on chemistry error flag 0...

Страница 143: ...interval to t where t is in minutes MRt change precal time interval to t where t is in minutes MOt change postcal time interval to t where t is in minutes MSs change monitor station number to s Abort...

Страница 144: ...9 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 145: ...Glutamate 0 73 731 0 07 5 00 146 L Glutamine 0 73 731 0 07 5 00 146 L Lactate 0 50 500 0 05 5 62 89 Lactose 5 00 5000 0 50 14 62 342 Methanol 1 00 1000 0 10 31 25 32 Peroxide 0 60 600 0 06 17 65 34 S...

Страница 146: ...and denominator 3 Multiply numbers mg L 2 50 g L 2 50 g L 1000 mg g 2 50 1000 mg L 2500 mg L w v 2 50 g L 2 50 g L 0 1 L dL 2 50 0 1 g dL 0 250 g dL 0 250 Note g dL is g 100ml or percent mmol L 2 50...

Страница 147: ...tamine 1 17 1 169 0 117 8 00 146 1 11 1 111 0 111 7 60 2 80 2 806 0 281 31 5 L Lactate 2 67 2 672 0 267 30 0 89 2 54 2 539 0 254 28 5 26 25 26 250 2 63 76 8 Lactose 25 00 25 000 2 50 73 1 342 23 75 23...

Страница 148: ...01 0 34 89 Lactose 342 Methanol 0 05 50 0 01 1 56 32 Sucrose 0 10 100 0 01 0 29 342 NOTE Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution Cho...

Страница 149: ...L 180mL 2 50 g L 120mL 2736 L Glutamine Standard 5 00 mmol L 250mL 2737 L Glutamine Standard 8 00 mmol L 156mL 2755 L Glutamate Standard 5 00 mmol L 250mL 2756 L Glutamate Standard 10 0 mmol L 125mL...

Страница 150: ...2702 membrane 4liters 2787 Ethanol Buffer Kit use with 2786 membrane 4liters 2363 Potassium Ferrocyanide 1000 mg dL 125mL 2392 NaCl Solution sodium chloride 30mL Reagent packaged dry Makes specified...

Страница 151: ...s about 60 seconds The information on the printout completely describes the system parameters whether using default settings or settings that you configured for your particular application You can lea...

Страница 152: ...D OFF MonStation 1 Sip Height Medium SamInterval 0 Min Autostandby 2 Hr PreCal 0 Min TT StartPos 0 PostCal 0 Min TT in Run 0 Purge Time 0 Sec TT FluidDet ON RS 232 MONITOR Baud rate 9600 MonStation 1...

Страница 153: ...L Lactate Unstable B L Lactate 4 82 g L IB current 1 79 nA IB 2 11 nA PL current 11 63 nA PL current 18 65 nA FB current 1 51 nA Slope 0 69 nA min Base shift 2 65 End Point 30 Sec Slope 1 42 nA min W...

Страница 154: ...D 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 155: ...or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures reorient the rec...

Страница 156: ...E 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 157: ...ny way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILI...

Страница 158: ...onsibility of the sender When service is required either at the user s facility or at YSI the following steps must be taken to insure the safety of our service personnel 1 In a manner appropriate to e...

Страница 159: ...d the product completely with packing material 5 Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Zip Country Phone Model No of Device Lo...

Страница 160: ...F 4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 161: ...G 1 Figure G 1 Line Power Cord and Plug Wiring Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 162: ...G 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 163: ...rd all tubing New tubing is provided in preventive maintenance kit Empty waste bottle and wash with disinfecting agent Remove Sample Chamber Sipper Test Tube Holder and probes according to instruction...

Страница 164: ...H 2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 165: ...or the purposes of fluid detection and human safety This circuitry may be susceptible to ESD ESD to the sipper needle may cause instrument malfunction 3 Users should note that RS232 communications may...

Страница 166: ...rings Ohio 45387 USA 062521 937 767 7241 800 659 8895 Fax 937 767 8058 A23781L Info ysi com www YSI com July 2000 2000 YSI Incorporated Artisan Technology Group Quality Instrumentation Guaranteed 888...

Страница 167: ...uipment Have surplus equipment taking up shelf space We ll give it a new home Learn more Visit us at artisantg com for more info on price quotes drivers technical specifications manuals and documentat...

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