YSI 2700D, Руководство пользователя

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4-7
$
This is a direct reading of sucrose in solution at the enzyme sensor. Three enzymes are co-
immobilized in the YSI Sucrose Membrane: Invertase, Mutarotase, and Glucose Oxidase.
S H
2
O
Invertase
α
-D-G (Fructose)
α
-D-Glucose
Mutarotase
β
-D-Glucose
β
-D-G O
2 Glu Oxidase
H
2
O
2
+ D-Glucono-
δ
-Lactone
Through this chain of reactions the moles of hydrogen peroxide liberated is directly
proportional to the moles of sucrose.
System Buffer YSI 2357
Calibrator Std YSI 2780 (5.00 g/L Sucrose)
Linearity Std YSI 2778 (25.0 g/L Sucrose)
Black Probe YSI 2703 Sucrose Membrane
White Probe n/a *
Sample Size 25
µ
L
Cal Station Station #1 Cal Well
Black Probe Parameters
Chemistry
Sucrose
Unit of Conc g/L
Cal value 5.00
End Point 30 sec
* If you have a dual channel unit, either Black or White, or both Black and White probes can be
configured for this chemistry.
Note: See Appendix A if concentration unit conversion is required.
Special Considerations:
» If sample dilution is required, use reagent water or 2357 buffer.
» The sample must be dextrose-free, or at least contain levels low enough not to
interfere with the sucrose reading. Since the sucrose membrane contains glucose
oxidase, dextrose will produce a probe signal. See Section 4.16 to measure dextrose
and sucrose simultaneously.
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