YSI 1500 Sport, Руководство пользователя, Страница: 112 / 134

12 12 12 12----2 2 2 2
inaccuracy of determining an athletes actual lactate level, which can affect the
appraisal of the athletes performance.
IMPORTANT: YSI strongly recommends that you use the YSI 1515 IMPORTANT: YSI strongly recommends that you use the YSI 1515 IMPORTANT: YSI strongly recommends that you use the YSI 1515 IMPORTANT: YSI strongly recommends that you use the YSI 1515 Cell Cell Cell Cell
Lysing Agent. Lysing Agent. Lysing Agent. Lysing Agent. Without total cell lysis (breaking apart of cell walls), results
obtained on whole blood can vary significantly depending on individual
hematocrit levels, on whether unintended partial cell lysis takes place, on
temperature changes, and the amount of time that elapses from the time of
sample collection until the analysis is completed.
The presence of the lysing agent in the buffer solutions eliminates these
problems, and also allows the results to be compared directly to more traditional
total blood lactate laboratory techniques.
Using the YSI 1515 Cell Lysing Agent
Using the YSI 1515 Cell Lysing Agent Using the YSI 1515 Cell Lysing Agent
Using the YSI 1515 Cell Lysing Agent
If your sample measuring protocol requires the total lysis of blood cells, or if
you are going to preserve the samples with the YSI 2315 Preservative Tube Kit
for later analysis, another step is required in the reagent preparation process
explained in Section 3.3 Reagent Preparation.
Buffer Solution Buffer Solution Buffer Solution Buffer Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized water or distilled water. Add one package of
YSI 2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Add one packet of YSI 1515 Cell Lysing Agent and stir.
Pour the solution into the instrument Buffer Bottle.
Reference Solution Reference Solution Reference Solution Reference Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized water or distilled water. Add one package of
YSI 2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Add 4.5 mL of YSI 1530 30 mmol/L Calibration Standard, and stir as necessary.
This should be measured out with the graduated syringe supplied in the 1504
Starter Kit. Replace the bottle lid and shake the bottle for 15 seconds to insure
complete mixing. It is important for reliable results that the reference
. It is important for reliable results that the reference . It is important for reliable results that the reference . It is important for reliable results that the reference
solution is completely mixed. solution is completely mixed. solution is completely mixed. solution is completely mixed.
Add one packet of YSI 1515 Cell Lysing Agent and stir.
Pour this solution into the Reference Bottle.