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Troubleshooting
FAULT
Possible
Cause
Remedy
UltraPath 2
sample
cell is leaking.
Nuts and
ferrules are not
tightened.
1 . Check and tighten all Nuts and ferrules at the
sample injector and the
sample output of the UltraPath 2 cell. Ensure that
the ferrules are installed properly; hand tighten the
nuts.
2 . If the ferrules are deformed, exchange them.
3 . If the tubing end of the Injector is deformed and
the fitting ferrule does not
have a snug fit, shorten the tubing 2-4 mm using a
sharp razor blade.
4 . Exchange Injector if a tight seal cannot be
obtained at the sample inlet.
5 . Exchange the silicone tubing assembly, if no seal
can be obtained at the sample outlet.
Sample solution is
not drawn into
the UltraPath 2
sample cell.
Nuts and
Fittings are not
tightened.
Remove fittings and insert them into the bulkhead
fittings and hand-tighten them again.
Sample solution is
not drawn into
the UltraPath 2
sample cell.
Silicone tubing
is not pressed
at the rollers in
the pump head.
Silicone tubing is not properly set in the PeriStar
Pro pump head. See PeriStar Pro
instruction manual how to set ins tall silicone
tubing in the pump head
The pump is not
drawing liquid,
even silicone
tubing is inserted
directly into the
sample vial.
Silicone tubing
walls stick to
each other.
The walls of silicone tubing may stick to each other
as the tubing gets older and if the tubing is not
removed from the pump head, when not in use.
Check and exchange the silicone pump tubing;
further, ensure that the pressure applied by the
pump head of the PeriStar Pro tubing is optimized
(see PeriStar Pro manual for details).
There is no light
or very little light
at the TIDAS S
300 UV/VIS
detector
The sample cell
is not filled with
solution
Fill the sample cell with reference solution and
check again
There is no light
or very little light
at the TIDAS S
300 UV/VIS
detector
Integration time
is not setup
correctly
Check in the calibration sheets, which integration
time is required for the selected pathlength and set
the appropriate time in the Spectralys software.