Block Cooler V1.0
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B. Introduction
Block Cooler System (CB-1) is a standard cooling unit for laboratory use. The compact
cooling unit temperature ranging 0 – 75
℃
allows customizing settings for specific temperatures
or utilizing the nine frequently used pre-set temperatures for various molecular biology or
chemistry applications. The universal 48-well aluminum block is a single block designed to suit
various types of microtubes without the need of the multiple blocks. An applicable samples for
block cooler accordingly for various temperatures is given in the table below:
Selectable Temperature
Range
Recommended Applications
0 °C
Replace the ice buckets for samples & enzymes storage;
4 °C
Sample cooling & storage during operation –
i.e: Store nucleic acid at 4°C in long PCR at final stage, DNA
purification kit rinsing and sample with 70% ethanol at 4°C,
hybridization process when adding polyA to hold at 4°C in nucleic
acid.
cDNA synthesis experiment –
i.e: Placing oligonucletides or polymerase at 4°C.
Pre-thaw the primary antibody for continuous uses –
i.e: western blotting procedure.
Enzyme reaction –
i.e: ligation steps of gene construction.
Cell lysis experiment –
i.e: Resuspend the protein in lysis buffer at 4°C.
General immunoprecipitation procedure.
16 °C
Enzyme reaction –
i.e: Restriction enzyme activity for DNA ligation.
25 °C
Biological response assay.
Enzyme catalysis activity reaction.
In long PCR reaction at final stage “hold”.
