VWR International VWR 10, Инструкция по эксплуатации

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Wait for at least a few seconds before releasing the Push-button to expel any residual liquid from
the tip. While removing the pipette, draw the tip along the inside surface of the vessel.
5/ Eject the tip by pressing firmly on the tip-ejector button.
General guidelines for good pipetting:
a. Make sure that you fit new tips.
b. Each new tip should be pre-rinsed with the liquid to be pipetted.
c. When aspirating, keep the tip at a constant depth below the surface of the liquid
d. Change the tip before aspirating a different liquid, sample, or reagent.
e. Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:
• Never turn the pipette upside down,
• Never lay the pipette on its side when there is liquid in the tip(s),
g. The VWR charging Stand Adapter (refer to chapter 2) is recommended for use with the VWR
Carrousel Pipette Stand to store the pipette in the vertical position.
h. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip
several times before use in order to reach equilibrium between the temperatures of the liquid and
the pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
chapter 14
k. The pipette can be used b 4 °C and
+ 40 °C, but the specifications may vary
(refer to chapter 17).
l. Do not pipette liquids having temperatures
above 50 °C or below 4 °C.
Extreme temperature
can affect accuracy
and precision