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Filling and Sealing the Vials
The 1.5 mL vials, as well as the conical vials, can best be filled
by means of a narrow-end pipette to allow air to escape while
filling the vial.
NOTE:
Do not fill vials past the shoulder. Sample will be forced into the air
needle, risking extra cross-contamination of samples.
It is important that the seal be airtight to maintain a constant
pressure on the vial for air bubble prevention and reproducible
injections.
Leaking vial septa will impair reproducibility when doing loop
overfill with headspace pressure.
NOTE:
Do not reuse a vial without replacing its septum.
Loading the Sample Tray
To allow remote loading of the sample tray, the sample tray
consists of four removable segments that fit in one unique
position of the tray holder.
Use the STEP key (see
Operation
on page 17) to rotate the tray
(functioning only when the ProStar 400 is in READY status).
CAUTION
Do not rotate the tray manually; you may damage the tray drive mechanism.
Positioning of vials
Sample vials should be placed in sequential positions on the
tray. Empty spaces between vials of a sample series are
allowed, since the ProStar 400 can detect empty positions. After
detecting an empty place, the AutoSampler will continue with the
next position.
Содержание ProStar 400
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