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9) Water Sampling (for Flange Models)
UV Superstore, Inc. recommends a program of water sampling to ensure delivery of the desired water
quality. While the frequency of sampling will depend upon site conditions and requirements, the user should
establish a disciplined program.
a) Sampling Valves
UV Superstore, Inc. recommends installation of sampling valves on the inlet and outlet sample ports
located on the ultraviolet unit risers. The wetted material should be 316 stainless steel construction. A
minimal length of thread connections of the sample valve port should be maintained to reduce the dead
volume on the upstream side of the valve. The inside diameter of the sample valve must not exceed
one-quarter inch (1/4") to insure proper velocity. Any valve used for sampling should be constructed in a
manner to reduce or prevent the retention of bacteria within its internal surfaces. The valve should also be
easy to sanitize.
b) Sampling Procedure
1) Prior to sampling the water, acquire an adequate supply of bacteriological monitors, total count water
samplers, pre-sterilized plastic bottles within pre-sterilized plastic bags from a water testing laboratory.
2) With the ultraviolet system operating, open the sample valve fully and flush for at least one minute at full
fast flow. Then, close the valve.
3) Sanitize the valve and sampling port in accordance with the proper standards established for the specific
valve installed. Isopropyl alcohol 70% to 90% is recommended.
4) Fully open and flush the valve again to remove any contaminants. The flushing time sequence may run
from a minimum of three to five minutes. Do not reduce the water flow before taking the water sample
because the back of the valve (not previously exposed) may contain contaminates. If you reduce the flow
prior to taking a sample, continue to flush as before.
5) When sampling, take the measurement at the center of the stream in order to reduce the possibility of
bio-fouling.
6) Follow ASTM or your bacteriological monitor manufacturer guidelines when using that monitor, with a
total count water sampler and a pre-sterilized plastic bag, to sample the in-line.
7) Carefully fill the pre-sterilized bottle from the bottom up allowing the water stream to overflow and flush
the bottle for a minimum of one minute.
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