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Turner Cyclops 6K Скачать руководство пользователя страница 19

Cyclops Submersible Sensor

998-2100

Rev. 2.9

Page 19

Appendix F: Linear Range, Quenching and Temperature Considerations

The linear range is the concentration range in which the

fluorometer’s output is directly

proportional to the concentration of the signal. The linear range begins with the
smallest detectable concentration and spans to an upper limit (concentration) that is
dependent upon the properties of the material, filters used, and path length.

A non-linear relationship is seen at very high concentrations where the signal does not
increase at a constant rate in comparison to the change in concentration (see figure
below). At even higher concentrations, the signal will decrease even though the
sample concentrations are continuing to increase. This effect is known as “signal
quenching”.

Linearity can be checked by diluting a sample 1:1 or some other convenient ratio. If
the sample is still in the linear range, the reading will decrease in direct proportion to
the dilution. If the reading does not decrease in direct proportion to the dilution, or if
the reading increases, the sample is beyond the linear range.

Temperature Considerations

Fluorescence is temperature sensitive. As the temperature of the sample increases,
the fluorescence decreases. For greatest accuracy, record the sample temperature
and correct the sensor output for changes in temperature.

For further information on how temperature, light, water quality and the physiological
state of the algal cells can all affect the measurement of chlorophyll, please refer to
the application

section of Turner Designs’ website.

Graph showing Linear and
Quenching Regions of the
sample

’s response

Sample Concentration

Fl

uo

rom

e

te

r

Rea

di

ng

Fluorometer Response Curve

Sample

Quenching Region

Sample

Linear Region

Graph showing Linear and
Quenching Regions of the
sample

’s response

Sample Concentration

Fl

uo

rom

e

te

r

Rea

di

ng

Fluorometer Response Curve

Sample

Quenching Region

Sample

Linear Region

Sample Concentration

Fl

uo

rom

e

te

r

Rea

di

ng

Fluorometer Response Curve

Sample

Quenching Region

Sample

Linear Region

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Содержание Cyclops 6K

Страница 1: ...User s Manual May 18 2018 P N 998 2100 Revision 2 9 TURNER DESIGNS 1995 N 1st Street San Jose CA 95112 Phone 408 749 0994 FAX 408 749 0998 ...

Страница 2: ...bration for Chlorophyll Cyclops 3 3 2 Direct Concentration Calibration for Turbidity Cyclops 10 10 4 Maintenance and Warranty 4 1 Maintenance 11 4 1 1 Rinsing 11 4 1 2 Care for the bulkhead connector 11 4 1 3 Cleaning the Optics 11 4 2 Warranty Terms 11 4 3 Warranty Service 12 4 4 Out of Warranty Service 12 Appendices A Specifications 14 B Recommended Measurement Practices 15 C Wiring Guide 16 D P...

Страница 3: ...roduct proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turn...

Страница 4: ...ti parameter systems from which it receives power and delivers a voltage output proportional to the concentration of the fluorophore particle or compound of interest The Cyclops voltage output can be correlated to concentration values by calibrating with a standard of known concentration Effective April 1 2017 all Cyclops 7 shipping will be Cyclops 7F Updated to have a faster settling time Cyclops...

Страница 5: ...ation Letter Plastic or Titanium housings recommended for highly corrosive environments or long term deployments Titanium Cyclops 7F P N 2110 000 Identification Letter T Plastic Cyclops 7F P N 2118 000 Identification Letter Plastic Cyclops 7F with Titanium connector P N 2118 000 Identification Letter T Titanium Cyclops 7 P N 2100 000 Identification Letter T Plastic Cyclops 7 P N 2108 000 Identific...

Страница 6: ... recommend use of the shade cap as it provides a fixed distance for sample measurement and minimizes effects from ambient light Cyclops Stainless Steel and Titanium P N 2100 701 Cyclops Plastic P N 2100 708 Cyclops 6K P N 2160 700 Cyclops 7 Chlorophyll Standard Solution Blue Excitation p n 2100 320 Note Not for use with Cyclops 6K Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Ph...

Страница 7: ...yclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 3 3 6 6 4 4 2 2 1 5 5 5 2 4 4 6 1 3 Cyclops pins wires 3 4 2 1 5 6 6 Cyclops pins wires 3 4 2 1 5 6 6 3 4 2 1 5 6 6 3 3 4 4 2 2 1 5 5 6 6 Cyclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 3 3 6 6 4 4 2 2 1 5 5 5 2 4 4 6 1 3 Cyclops pins wires 3 4 2 1 5 6 6 Cyclops pins wires 3 4 2 1 5 6 6 3 3 4 4 2 2 1 5 5 6 6 Cyclops Pigtail cable holes 3 3...

Страница 8: ...it to a laboratory where you have the Cyclops connected to a multi meter and DC power source see Figure 1 2 Pour the water sample into a clean glass beaker and submerge the optical end of the Cyclops see Appendix B for Recommended Measurement Practices for using your Cyclops in the Lab for how best to accomplish these steps 3 Activate the X10 gain setting see Wiring Guide Appendix C if you believe...

Страница 9: ...ure maximizes the performance of Cyclops sensors allowing users to detect a broad range of concentrations obtain the best resolution and read minimum detection limits without having to rewire or manually change the sensor s sensitivity Turner Designs manufactures the DataBank Handheld Data Logger see Appendix H which has the auto gain feature and other functions that maximize the performance of Cy...

Страница 10: ...between the standard and the sensor s voltage response Use the equation from step 4 of section 3 3 to calculate chlorophyll estimates as µg L in the calibrated gain Note This calibration is an approximation Actual µg L estimates will vary depending on factors such as speciation light history algal stresses pigment ratios etc 3 3 2 Direct Concentration Calibration for Turbidity Cyclops Turner Desig...

Страница 11: ...s models are the ONLY Cyclops sensors that can be calibrated with Quinine Sulfate standards made in Hydrosulfuric Acid All other Cyclops models CANNOT be used in Hydrosulfuric Acid 4 2 Warranty Terms Turner Designs warrants the Cyclops and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner De...

Страница 12: ...urned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Design...

Страница 13: ...Cyclops Submersible Sensor 998 2100 Rev 2 9 Page 13 ...

Страница 14: ...Emitting Diode Excitation Wavelength Visible Chl RWT PC PE F UV CDOM FDOM Oil OB RF PTSA IR Turbidity Detector Photodiode Detection Wavelength 300 1100 nm Warm up time Cyclops 7F 1 second Cyclops 7 5 seconds Housing Material Cyclops Standard 316 Stainless Steel Optional Titanium Optional Plastic Housing Material Cyclops 6K Titanium Dimensions Cyclops Stainless Steel and Titanium L 5 74 in 14 48 cm...

Страница 15: ...ing a glass container place the container on a non reflective black surface 3 Ensure that the sensor is more than 3 inches above the bottom of the container 4 Ensure that the sensor is in the center of the container and has more than 2 inches clearance between the cirumference of the sensor and the inside surface of the beaker 3 inches Dark Black Non Reflective Surface 2 inches all round Calibrate...

Страница 16: ...ive Connection Blue 5 X10 Gain Medium Sensitivity See table below Yellow 6 X100 Gain High Sensitivity See table below Gain Switching Table Gain 10 Blue Gain 100 Brown Gain Not connected Not connected X 1 Connected to analog ground Not connected X 10 Not connected Connected to analog ground X 100 Cyclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 Cyclops pins wires 3 4 2 1 5 6 6 Cyclops Pigtail ...

Страница 17: ...4 2 1 5 6 6 3 4 2 1 5 6 6 3 3 4 4 2 2 1 5 5 6 6 Cyclops pins wires 3 4 2 1 5 6 6 3 3 4 4 2 2 1 5 5 6 6 3 3 4 4 2 2 1 5 5 6 6 3 3 4 4 2 2 1 5 5 6 6 Cyclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 Cyclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 3 3 6 6 4 4 2 2 1 5 5 5 2 4 4 6 1 3 Cyclops Pigtail cable holes 3 6 4 2 1 5 5 2 4 4 6 1 3 3 3 6 6 4 4 2 2 1 5 5 5 2 4 4 6 1 3 Cyclops Pigtail cab...

Страница 18: ... Also see Setting the Gain section 3 2 for more information on Static Gain Control Method 2 Dynamic Gain Control If you have a Data Collection System DCS that has programmable outputs you can use them to control the Cyclops gain settings Following are three common output types found in DCSs and how to connect them to the Cyclops Refer to your DCS manual to determine which is appropriate For those ...

Страница 19: ...ll decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor outpu...

Страница 20: ...IR which is not visible 2 Is there voltage output The multi meter should be reading some voltage 0 VDC 3 Does the voltage output change Move the light source closer to your hand or a surface and check if the voltage output increases 12 00 015 DC Power Supply LED Light Output PSU Positive Connection Red Supply Ground 0 VDC Black 3 52 Multimeter 10 VDC Signal Output Orange Analog Ground Green Blue T...

Страница 21: ... for different sampling protocols Multiple Site Measurements measure fluorescence at different locations within your water system or across many systems GPS enabled units provide latitude longitude data per location Profiling purchase extended cables to allow for vertical profiling Monitoring deploy the Cyclops to a fixed location or depth and monitor the signal over time set up logging to capture...

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