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Trace2O AquaSafe WSL25 Plus, Инструкция по эксплуатации

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1.0 Introduction 

The AquaSafe® Single Incubator is a portable incubator for the incubation of microbiological 

samples prepared using the membrane filtration method.  The incubator is primarily designed to 

be used with the supplied 54mm x 3.5mm aluminium petri dishes which are suitable for 47mm 

membrane filter pads, but the incubator can also be used with 55mm pre-prepared plastic petri-

dishes.  

The incubator is supplied with 25 Petri dishes as standard but has the capacity for more due to the 

Petri-Lok® system. 

The incubator has the option to run 37°C, 44°C and user defined temperature profiles for periods 

of 1 – 24 hours.   

 

 

 

Содержание AquaSafe WSL25 Plus

Страница 1: ...AquaSafe WSL25 Plus Water Safety Laboratory Instruction Manual...

Страница 2: ...Contents Section 3 Single Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 Turbidity Tube Instructions Section 6 Comparator Instructions Section 7 Pocket pH Tester Instruct...

Страница 3: ...comprises the following Integrated single chamber digital incubator complete with all accessories and consumables for carrying out 300 tests for Faecal and Total Coliforms Comparator complete with ac...

Страница 4: ...Section 2 Kit Contents 2 1 3 4 Under tray 8 9 12 13 14 15 16 17 18 19 Under charger 10 11 6 5 7 a a a a b b c d e f g h i k j...

Страница 5: ...on Tablet Bags Whirl Pak 14 1 Comparator Chlorine Disc 14 1 Comparator Ammonia Disc 14 1 Comparator Nitrate Disc 14 1 Comparator Nitrite Disc 15 4 Comparator Cells 16 1 Nitrate Test Tube 17 1 Test Tub...

Страница 6: ...ck 3 Chlorine Free Total Reagents 100 Pack 3 Ammonia Reagents 100 Pack 3 Nitrate Reagents 100 Pack 1 pH 4 01 Calibration Solution 1 pH 7 00 Calibration Solution 1 EC 1413 S Calibration Solution 2 Ster...

Страница 7: ...Section 3 AquaSafe Micro biological Incubator Instruction Manual...

Страница 8: ...5 3 3 2 Start Screen Figure 1 1 16 3 3 3 37 C Program Fig 1 2 17 3 3 4 44 C Program Fig1 3 18 3 3 5 User defined Program Fig 1 4 19 3 3 6 Resetting the timer Fig 1 5 19 3 3 7 Incubation cycle time set...

Страница 9: ...with the supplied 54mm x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator is...

Страница 10: ...t become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which inco...

Страница 11: ...if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection o...

Страница 12: ...used cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from...

Страница 13: ...sting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist lock into place The incubator has a power connection socke...

Страница 14: ...ilure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the previous incubation cycle can be restarted from where it was left by pressing restart This will be discussed later The...

Страница 15: ...sequence 20 9 C 24 00 OFF MEM 10 5v Select Option Restart New Run 20 9 C 24 00 37 MEM 10 5v 20 9 C 24 00 44 MEM 10 5v 20 9 C 24 00 U MEM 10 5v 20 9 C 24 00 New SEL 10 5v 20 9 C 24 00 Cal ADJ 10 5v 20...

Страница 16: ...e 1 1 This screen shows the current status of the incubator In the state shown the incubator is in idle mode and not running Current Temperature Time Remaining Incubator Status Battery Voltage Mode 20...

Страница 17: ...hold the middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 Once the chamber has reached the pre set temperature the timer will start to countdown If for any reason...

Страница 18: ...is described in section 3 3 7 Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature wi...

Страница 19: ...middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 3 3 6 Resetting the timer Fig 1 5 In this mode pressing the middle switch will display the following screen which...

Страница 20: ...middle switch to save the settings The display will return to the one shown in Fig 1 1 3 3 8 User defined temperature setting Fig1 7 In this mode the user defined incubation temperature can be adjust...

Страница 21: ...Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step thro...

Страница 22: ...et conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be...

Страница 23: ...e rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contac...

Страница 24: ...d Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd...

Страница 25: ...Section 4 AquaSafe Membrane Filtration Instruction Manual...

Страница 26: ...29 SECTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 30 SECTION 4 SAMPLING 31 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 33 SECTION 6 ASEPTIC PROCEDURES 34 SECTION 7 PROCESSING SAMPLES FOR COLIFORM A...

Страница 27: ...FILTRATION APPARATUS 1 Hand Vacuum Pump 2 Filtrate Flask Waste Beaker 3 Sampling Cup and cable 4 Graduated Aluminium Funnel 5 Membrane Support Holder 6 Sealing Gaskets 7 Glass Sintered Disc Membrane...

Страница 28: ...and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screw...

Страница 29: ...cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place b...

Страница 30: ...nge to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised wa...

Страница 31: ...on bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination The dechlorination tablets are pre placed...

Страница 32: ...ntil the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by i...

Страница 33: ...in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required...

Страница 34: ...ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample...

Страница 35: ...is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the...

Страница 36: ...e the pad with prepared broth Loosen the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass me...

Страница 37: ...unnel up to the 100 ml graduation Connect the hand vacuum pump to the filtration unit base ensuring the arrow is orientated to the direction of desired air flow and pump in a controlled fashion to suc...

Страница 38: ...he sample number place date time etc Place the petri dish into the rack Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that when the l...

Страница 39: ...hin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of...

Страница 40: ...s 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sam...

Страница 41: ...Section 5 AquaSafe Turbidity Tube Instruction Manual...

Страница 42: ...turbidity tube from their position in the foam recess at the front of the AquaSafe Kit Align the two halves of the turbidity tube so that the graduations are easily visible then push together the gre...

Страница 43: ...tively fill the tube then slowly pour small portions away until the moment that the Secchi marker becomes visible from the top of the tube Hold the tube vertically and identify the water level The tur...

Страница 44: ...Section 6 AquaSafe Comparator Instruction Manual...

Страница 45: ...ator Equipment Comparator 2 x 10ml cells Stirring rod Ammonia Nos 1 2 tablets Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents before t...

Страница 46: ...No 1 tablet straight from the foil to the water sample in the second cell Crush the tablet using a clean stirring rod until no large pieces are visible Add one Ammonia No 2 tablet straight from the fo...

Страница 47: ...clean The tablets must be added in the correct sequence Ammonia No 1 tablet will only dissolve fully after Ammonia No 2 tablet is added The tablets should be added direct from the foil avoiding contac...

Страница 48: ...rator Equipment Comparator 2 x 10ml cells Stirring rod DPD No 1 DPD No 3 Tablets Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents befor...

Страница 49: ...parator as shown in fig 1 1 This is the reference cell Free Chlorine Sample preparation Add one DPD No 1 tablet straight from the foil to the water sample in the second cell Crush the tablet using a c...

Страница 50: ...reading on the disc Total Chlorine Sample preparation Remove the cell from the right side of the comparator Remove the lid and add one DPD No 3 tablet straight from the foil to the water sample Crush...

Страница 51: ...the reading on the disc Combined Chlorine mg L ppm Total Chlorine mg L Free Chlorine mg L Notes For best results rinse vials thoroughly between with deionised water Ensure that the outside of the via...

Страница 52: ...2 x 10ml cells Stirring rod Nitrate test tube Nitrate test powder Nitrate test tablet Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used c...

Страница 53: ...one Nitrate Test tablet straight from the foil to the nitrate test tube Do not crush the tablet Refit the cap and swirl vigorously for 1 minute Stand the tube upright allow contents to settle then inv...

Страница 54: ...foil avoiding contact with hands or surfaces Nitrite present in the sample will also react leading to a higher result To correct carry out a nitrite test C270 on the sample and subtract the result Ni...

Страница 55: ...mparator Equipment Comparator 2 x 10ml cells Stirring rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents before the...

Страница 56: ...n in fig 1 1 This is the reference cell Sample preparation Add one Nitrite LR tablet straight from the foil to the water sample in the second cell Crush the tablet using a clean stirring rod until no...

Страница 57: ...est results rinse vials thoroughly between with deionised water Ensure that the outside of the vials are clean The tablets should be added direct from the foil avoiding contact with hands or surfaces...

Страница 58: ...Section 7 HydroLite HL101 Pocket pH Tester Instruction Manual...

Страница 59: ...r is fully functional the tip of the probe must be submerged in liquid during when not in use If left to dry out the electrode could be damaged and therefore affect the testing Trace2o uses a potassiu...

Страница 60: ...old Power the unit ON OFF Freezes the currently displayed value for recording press the key again to resume measuring When in calibration mode exits calibration and returns to measurement mode Cal Pre...

Страница 61: ...compensation is enabled Prior to Use Remove the electrode protective cap from the unit If the membrane on the electrode dries out soak the electrode in 3M KCL solution or tap water for at least 15 mi...

Страница 62: ...able reading Disable Auto Off Automatically turn off the unit Disable Reset Restore factory settings Disable Changing the default parameters 1 Press and hold the CAL key for 3 seconds to enter the set...

Страница 63: ...abled If the auto hold function is enabled the unit will automatically sense a stable endpoint reading and freeze the reading in the display If you disable this function the unit allows user to freeze...

Страница 64: ...es and selected parameters will be reset 10 Press CAL key to enable or disable the reset function Press ENTER key to confirm the unit returns to measurement mode EXIT THE SETUP MENU During the setup m...

Страница 65: ...r the electrode is replaced To ensure optimum accuracy regular calibration is recommended Do not reuse calibration solution after calibration contaminants in solution will affect the calibration and e...

Страница 66: ...stabilize the display shows electrode slope and END 2 point calibration is completed 3 POINT CALIBRATION not required for normal operation 3 1 Ensure that 3 point calibration is selected in the setup...

Страница 67: ...ir the solution gently 3 6 Press ENTER key to confirm Wait for the measured value to stabilise the display shows electrode slope and END Calibration is completed EXIT THE CALIBRATION During the calibr...

Страница 68: ...Press CAL key to enter the temperature calibration mode 4 Press CAL key again to set temperature value Resolution 0 5o C 5 Press ENTER key to confirm the display shows next option 6 Press ON OFF key...

Страница 69: ...the pH electrode membrane wet Ensure that the electrode is thoroughly washed with distilled water after each use During extended periods of disuse store the sensor with electrode storage solution DO N...

Страница 70: ...de dried out Soak the electrode in 3M KCL solution or tap water for 10 minutes Measured value is out of range Check whether the electrode membrane is clogged dirty or broken Incorrect pH buffer soluti...

Страница 71: ...Power Off Manual or Automatic 8 minutes after last key pressed Sensor Type Standard pH Electrode Order Code HL101ELEC Power Requirements 2 1 5V AAA Batteries Dimensions 185 L 40 Dia mm Weight 100g...

Страница 72: ...rkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com The information in this document is subject to change without notice Copyright Trace2o Limited 2016 All rig...

Страница 73: ...Section 8 HydroLite HL102 Pocket EC Tester Instruction Manual...

Страница 74: ...mbols to describe the function of each key Map of keys and functions KEY FUNCTION On Off Hold Powers the unit ON OFF Freezes the currently displayed value for recording press the key again to resume m...

Страница 75: ...frozen ATC Indicates temperature compensation is enabled Prior to Use Remove the electrode protective cap from unit Soak the electrode for a few minutes in tap water to remove dirt and oil stains on...

Страница 76: ...Automatically freezes a stable reading Disable Auto Off Automatically turn off the unit Disable Reset Restore factory settings Disable SETTING THE DEFAULT PARAMETERS 1 Press and hold the CAL key for 3...

Страница 77: ...If the auto hold function is enabled the unit will automatically sense a stable endpoint reading and freeze the reading in the display If you disable this function the unit allows user to freeze the...

Страница 78: ...freshwater samples 1413 S cm should be sufficient The unit will automatically detect these conductivity standard solutions and prompt the user to calibrate the meter When the calibration is done all n...

Страница 79: ...o start the calibration Wait for the reading to stabilise the display shows END The unit returns to measurement mode automatically Single point calibration is completed 2 POINT CALIBRATION 2 1 Ensure...

Страница 80: ...it prompts to continue with third point calibration 3 2 Repeat the above steps until the display shows END the unit returns to measurement mode Calibration is completed Note Performing the conductivit...

Страница 81: ...onductivity measurement mode Measurement Rinse the conductivity electrode with distilled water Immerse the electrode into the sample solution Stir the solution gently Wait for the reading to stabilise...

Страница 82: ...s for calibration standards you need to replace the electrode module 1 Twist the electrode collar counter clockwise pull the old electrode module away from the unit 2 Align the slot on the new electro...

Страница 83: ...the electrode module Specifications Conductivity Model HL102 Range 0 20 00 200 0 2000 S cm 20 00mS cm Accuracy 1 F S Resolution 0 01 0 1 1 Calibration Points 1 to 3 points Calibration Solutions 10 S c...

Страница 84: ...or To determine the TDS conversion factor use the following formula Factor Where Actual TDS value from the high purity water and precisely weighed NaCl or KCL reagent Actual Conductivity the measured...

Страница 85: ...rkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com The information in this document is subject to change without notice Copyright Trace2o Limited 2016 All rig...

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