3.6
Revised 07/12/11
SET UP
ADD PARAFFIN
(Embedding Module)
Do not use paraffin with a melting point
higher than 75°C (167°F).*
*or paraffin will not liquefy; the maximum temperature of
the paraffin chamber is 75 ± 2°C (167.0 ± 3.6°F)
WARNING: MOLTEN PARAFFIN IS HOT AND
MAY CAUSE BURNS. DO NOT OVERFILL
PARAFFIN CONTAINER.
1.
Open
Paraffin Chamber Lid.
2.
Add
approximately 4 liters of clean molten paraf-
fin (or equivalent volume of paraffin chips/flakes)
to the chamber.
Do not overfill.
NOTE:
2 parts dry flakes/chips = 1 part molten paraffin
3.
If using the transfer tray as the paraffin bath,
add equivalent of 50 mL of molten paraffin** to
either right or left chamber (whichever you select
depending on your work flow direction)
**added to increase heat conductivity between tray and
chamber
Place
transfer tray into the chamber (over 50 mL
molten paraffin).
4.
Add
paraffin to the paraffin bath to a depth of
about 13 mm (0.5 in), or about .9 liter (.24 gal)
molten paraffin or equivalent in dry chips/flakes.
4.
NOTE:
The paraffin bath may be either the right or left
chamber or transfer tray (whichever you select).
4.
NOTE:
Change paraffin frequently to prevent build-up
of tissue debris.
5.
Pour
about 1 mL of clean molten paraffin into
each well in forceps holder.
6.
Place
one forceps into each well.
PLACE BASE MOLDS INTO
CHAMBER
Place base molds (sold separately, see Section 8) into
empty chamber (either right or left depending on
your chosen work flow direction).
3.6
