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Optical Microscopy Course Kit

Chapter 4: Kit Components

Rev C, October 1, 2020

Page 17

Fluorescence Emission Filter

1 x

FELH0550

550 nm Premium Longpass Filter

(Fluorescence Emission Filter)

1 x

SM1L05

Ø1" Lens Tube, 0.50" Long

1 x

SM1CP1

Internally SM1-Threaded End Cap

Back Focal Plane Camera

1 x

CS165CU (CS165CU/M)

Zelux™ 1.6 MP Color CMOS

Camera

1 x

SM1V10

Ø1" Adjustable Lens Tube,

0.81" Travel

1 x

SM1L10

Ø1" Lens Tube, 1" Long

1 x

LA1027

Ø1" N-BK7 Plano-Convex Lens,

f = 35.0 mm, Uncoated

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Содержание EDU-OMC1

Страница 1: ...Rev C October 1 2020 Optical Microscopy Course EDU OMC1 EDU OMC1 M User Guide...

Страница 2: ......

Страница 3: ...tter Assembly 34 5 2 11 Microscope Objective Nikon 35 5 2 12 Filter Holder Assembly 35 5 2 13 Polarization Mount Assembly 36 5 2 14 Fluorescence Filter Assembly 37 5 2 15 Diffuser Assembly 37 5 2 16 L...

Страница 4: ......

Страница 5: ...r on your device Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal Equipotentialit...

Страница 6: ...using will reach a temperature of up to 55 C Be careful not to touch the lamp housing during operation CAUTION The kit includes an IR LED LED940E which does not emit in the visible range The LED emiss...

Страница 7: ...Abbe theory of image formation and fluorescence filter use and proper microscope set ups including Kohler illumination and camera use This User Guide provides a starting point for working with this k...

Страница 8: ...se The Course Notes cover the theory for each lab s material The Lab Notes provide instructions and tasks for the students intended for 3 hour lab sections and provide the basis for each lab report Th...

Страница 9: ...focus of the kit is to learn how to set up an optical microscope and explore different microscope properties and configurations Starting in Labs 1 and 2 the students learn how to handle the optical r...

Страница 10: ...e comparison of data with theory Figure 4 Overview of the components on the optical rail 1 Camera 2 Objective 3 Sample 4 Condenser and Aperture Stop 5 Neutral Density Filter Wheel 6 Green Filter 7 Fie...

Страница 11: ...with a Camera and Focusing at Infinity Setting Up the Rail System Imaging and Resolution Measurement 3 Aberrations and Illumination Lens Orientation Illumination Spatial Coherence Color Chromatic Aber...

Страница 12: ...e Edge Spread Function ESF and the MTF 8 Contrast Methods and Abbe Theory Brightfield Darkfield and Signal to Background MTF and Contrast Phase Contrast 9 Fluorescence Microscopy Fluorescence and Fluo...

Страница 13: ...you should prepare the computer s and install the camera and spectrometer software You will find helpful information in the Appendices of the Lab Notes Course Notes and particularly the Instructor No...

Страница 14: ...theses Rail System 1 x Aluminum Breadboard 8 x 36 x 1 2 20 cm x 90 cm x 1 27 cm Single Density Taps 2 x RDF1 Rubber Damping Feet Set of 4 1 x XT34 100 1 x XT34 900 34 mm Construction Rails 100 mm and...

Страница 15: ...7 mm Optical Post Stainless Steel 2 50 mm Long 16 x TR3 TR75 M 1 2 12 7 mm Optical Post Stainless Steel 3 75 mm Long 6 x TR4 TR100 M 1 2 12 7 mm Optical Post Stainless Steel 4 100 mm Long 1 x LA1765...

Страница 16: ...M1RC SM1RC M Slip Ring for SM1 Lens Tubes 5 x LMR1 LMR1 M Lens Mount with Retaining Ring for 1 Optics 8 32 M4 Tap 1 x BA1 BA1 M Mounting Base 1 x 3 x 3 8 1 x FH2 Filter Holder 1 x FGL780M 780 1800 nm...

Страница 17: ...red Glass Bandpass Filter 335 610 nm 1 x HSLT2 Passive Heat Sink SM1 Lens Tube with External SM2 Threads 1 x SM1CP2 End Cap External SM1 Threads Collector and Field Stop 1 X LA1027 1 N BK7 Plano Conve...

Страница 18: ...25 mm VG9 3 mm Thick 1 x FBH520 40 25 mm Premium Bandpass Filter 520 nm CWL 40 nm FWHM Fluorescence Excitation Filter Condenser Optic 1 x SM1V10 1 Adjustable Lens Tube 0 81 Travel 1 x AC254 050 A 1 A...

Страница 19: ...ano Convex PCX Objective 1 x LA1951 1 N BK7 Plano Convex Lens f 25 4 mm Uncoated 1 x SM1RRC Extra Thick SM1 1 035 40 Threaded Retaining Ring Achromatic Objective 1 x SM05V05 1 2 Adjustable Length Lens...

Страница 20: ...al SM1 to Internal M25 Thread Adapter for Nikon Objective Beamsplitter 1 x LMR2 LMR2 M 2 Fixed Lens Mount 1 x EBP2 2 30 70 Beamsplitter Sample Camera 1 x CS165MU CS165MU M Zelux 1 6 MP Monochrome CMOS...

Страница 21: ...emium Longpass Filter Fluorescence Emission Filter 1 x SM1L05 1 Lens Tube 0 50 Long 1 x SM1CP1 Internally SM1 Threaded End Cap Back Focal Plane Camera 1 x CS165CU CS165CU M Zelux 1 6 MP Color CMOS Cam...

Страница 22: ...nt 1 x LED528EHP Epoxy Encased LED 525 nm 7 mW T 1 Qty of 5 Use with LEDMT1E Mount 2 x LED631E Epoxy Encased LED 635 nm 4 mW T 1 Use with LEDMT1F Mount 1 x LED940E Epoxy Encased LED 940 nm 18 mW T 1 Q...

Страница 23: ...Pack of 4 Colors 1 x LED Overhead Light 1 x Small LED Flashlight USB Rechargeable 1 x Clear Nail Polish 1 x Adhesive Printed Labels for Parts 1 x Roscolux Pattern Booklet Optical Filters 1 x Tape Disp...

Страница 24: ...32 Cap Screw 3 8 Long 2 SM1RC Slip Ring 1 4 20 Setscrew 1 2 Long 1 CFW6 Filter Wheel Thumbscrew 12 R2T Slip On Post Collar 8 32 Setscrew 1 4 Long 2 HKTS 3 16 Hex Key Thumbscrew 8 32 Nylon Tipped Setsc...

Страница 25: ...e the provided lip to align the two clamps exactly perpendicular 3 Open the locking screws on the side of the XT34HP XT34HP M and slide it onto the two rails Align the two rails against each other and...

Страница 26: ...oard edge Optics Subassemblies The following section shows how to assemble the optical components Handling lenses and optics in general requires the use of powder free gloves You can find these in all...

Страница 27: ...ure 9 Adhesive Label Sheet 5 2 1 Halogen Lamp Assembly 1 The halogen lamp has two sides and both are covered with end caps On one side is the collimation lens and on the other side is the bulb next to...

Страница 28: ...o the axis now will help during the labs a Tighten the setscrew sufficiently to hold well but not so tight it damages the ceramic socket 4 Take the FGB37M mounted filter 335 610 nm and screw the filte...

Страница 29: ...post 9 Place the lamp s post in the benchtop organizer The organizer also has through holes to hold the balldrivers included in the kit 5 2 2 Collector Lens Assembly 1 Use the SPW602 spanner wrench to...

Страница 30: ...ench to remove the retaining rings from the CFW6 CFW6 M filter wheel 2 Open the case of the ND05B filter and determine which side is metalized by looking through its edges as shown below Note the meta...

Страница 31: ...unt the one with no filter installed 6 Attach the 1 4 20 M6 setscrew 1 2 12 mm long to one of the central mounting holes 7 Attach the TR3 TR75 M post with the 1 4 20 M6 threaded side to it and remove...

Страница 32: ...n the same direction as the label on the housing of the filter away from the lip of the flip mount 3 Insert the FGV9 3MMT SP band pass filter in the other TRF90 TRF90 M mount and fix it with a retaini...

Страница 33: ...1 Screw the TR3 TR75 M post to the SM1RC SM1RC M slip ring and set it aside Check that the setscrew from the post does not protrude into the interior of the slip ring aperture where the SM1 tube will...

Страница 34: ...rew the SM1V10 variable lens tube into the previously assembled SM1M10 lens tube Lens focus position and locking will be set later during the alignment process The final assembly should look like the...

Страница 35: ...f the MS1S SP MS1S M stage while securing the adapter to it a IMPORTANT The adapter plate lip see figure should face away from the stage micrometer 3 Attach the XYF1 XYF1 M slide holder stage with an...

Страница 36: ...d stage is shown below 6 Place the assembly into the benchtop organizer for storage 5 2 8 PCX Objective Assembly 1 Remove the retaining ring from the LMR1 LMR1 M lens mount 2 Insert the LA1951 lens wi...

Страница 37: ...e 2 Screw the SM1A1 adapter onto the SM1D12C iris diaphragm Be careful not to grip and thus strain or damage the iris closing mechanism while tightening the adapter Be careful to instead hold the non...

Страница 38: ...s mount 2 Open the box of the EBP2 beamsplitter The beamsplitter has an exposed oxide coating on one side and is uncoated on the other side You can determine the coated side similar to the neutral den...

Страница 39: ...thread adapter in the optic mount 5 Place the assembly into the benchtop organizer for storage a Note store the phase contrast ring separately it cannot be used in this kit but can potentially be used...

Страница 40: ...en here 3 Remove the retaining ring from the short SM1L03 lens tube Then put the bare polarizer sheet in the SM1L03 lens tube and fix it into position with the retaining ring 4 Attach the lens tube to...

Страница 41: ...ns tube and put on an SM1EC2B end cap on one side of the tube and an SM1CP1 end cap on the other Putting end caps on both sides protects the delicate interference filter 5 2 15 Diffuser Assembly 1 Att...

Страница 42: ...rted wiggle it or unplug the LED again then reinsert The LED should go all the way into the mount 3 Use the provided Red label and add it to the SMR05 SMR05 M mount Alternately you can label the LED m...

Страница 43: ...the retaining ring from the SM1V10 adjustable lens tube 2 Add the F 150mm Ach label to the adjustable lens tube 3 Insert the AC254 150 A achromatic lens with the larger radius of curvature flatter sid...

Страница 44: ...d initial placement Final positioning after Lab 2 will be approximately 2 50 63 5 mm from the camera as shown halfway after additional lens tubes are added While exact slip ring positioning is not cri...

Страница 45: ...ts in Lab 2 If you want to set up the system immediately yourself then add one additional 1 25 4 mm SM1 lens tube to the camera mount above and start focusing the camera to infinity The steps are prov...

Страница 46: ...10 lens tube7 9 Add the label Color Camera to the side of the camera facing you when pointing to the right 10 Attach one of the SM1EC2B end caps to the adjustable lens tube to cover the lens 11 Place...

Страница 47: ...the post by using a cap screw 1 4 20 x 3 8 M6 10 mm through the counter sunk hole of the mounting base BA1 BA1 M attaching it to the 1 4 20 M6 internal thread in the end of the post 3 Place the assemb...

Страница 48: ...you should see a bulge which determines the UV printed side Put it with the bulge facing down into the SM1L03 0 3 7 6 mm lens tube Secure the optic with the retaining ring 3 Add the Zero Order Blocki...

Страница 49: ...tscrews are standard if you prefer thumbscrews as the authors do you must remove the setscrews from the post collars and insert the thumbscrews Subassemblies that should receive a slip on post collar...

Страница 50: ...s during the labs but not interfering with low light microscopy measurements or of other students The lamp s power supply is via USB or battery 4 x AA not included Note repeated pressing of the power...

Страница 51: ...30 0 mm N BK7 Plano Convex Lens f 100 0 mm Uncoated PCX Lens Used in Lab 1 Slip On Post Collars with Thumb Screws Rail Carriers Spanner Wrenches Lens Tissues Aluminum Foil Highlighters LED Flashlight...

Страница 52: ...e glass substrate Do not touch the glass with bare hands We advise storing them in the plastic boxes they are shipped in and to encourage users to treat them carefully and protect them from dust when...

Страница 53: ...t sets of wavelengths similar to a variety of fluorophores See the Fluorescence Graphs Tab Dimensions 25 4 mm x 76 2 mm 1 7 mm thickness Storage requirements protect the slides from light when not in...

Страница 54: ...rs to be installed Be sure to enable the installation of the USB drivers during the installation process Further information about the software installation and additional information about software o...

Страница 55: ...be found on the EDU OMC1 M product page on http www thorlabs com OMC website and in the Lab Notes material The Camera has a status LED indicator Blue Connected to USB 3 0 Port 35 FPS in full resoluti...

Страница 56: ...Notes Tips for Instructors Teaching the Labs PLEASE DO NOT copy the entire USB stick onto the student computer s If students obtain the Instructor Notes they will eventually end up on the web and the...

Страница 57: ...her Thorlabs products such as Pure OEM products that means assemblies to be built into a unit by the user e g OEM laser driver cards Components Mechanics and optics Left over parts of units disassembl...

Страница 58: ...on USA Canada and South America Thorlabs Inc sales thorlabs com techsupport thorlabs com UK and Ireland Thorlabs Ltd sales uk thorlabs com techsupport uk thorlabs com Europe Thorlabs GmbH europe thorl...

Страница 59: ......

Страница 60: ...www thorlabs com...

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