THORLABS EDU-OMC1, Руководство пользователя

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Optical Microscopy Course Kit Chapter 3: Brief Description
Page 8 MTN015225-D02
6
The Abbe Theory of Image
Formation (I)
- Imaging the BFP: Setting Up a
BFP-Imaging Camera
-
Diffraction and the BFP
- Spatial Filtering in the BFP
7
The Abbe Theory of Image
Formation (II)
- The Modulation Transfer
Function (MTF)
o
Incoherent MTF
o
Coherent MTF
- The Edge-Spread Function
(ESF) and the MTF
8 Contrast Methods and Abbe
Theory
- Brightfield
- Darkfield and Signal-to-
Background
-
MTF and Contrast
- Phase Contrast
9 Fluorescence Microscopy
- Fluorescence and
Fluorescence Imaging on the
Following Samples:
o
Autofluorescent Slides
o
Lens Tissue with
Fluorescent Markers
o
H&E (Fluorescent Eosin)
Stained Tissue Samples
10
Spectra and Filters
-
Light Spectra
- Filter Transmission and
Behavior
-
Spreadsheet-Based Problem
Set on Quantitative Filter
Selection
Oscillation
Corresponding Out
of Focus Area