Thermo Scientific SORVALL RC-5C PLUS, Руководство пользователя

Страница: 31 / 55

Operation SORVALL ® Centrifuges
4-6
Integrator Mode Operation
The integrator mode can be used in place of the timer to control the
length of the centrifuge run. The integral value (
∫ω
2 dt ) represents
the actual centrifuge force generated by the rotor accumulated over
time.
∫ω
2 dt is the integral value. The integral value is the area under the
curve (as represented by the grey area in the drawing above). The
larger the integral value, the more total centrifugal force (time and
g for ce) applied to the sample. The use of the integrator will
compensate for changes in rotor load and other factors that may
change the rate acceleration in the RC-5C PLUS. For a more detailed
explanation of the advantages of using the integrator, contact the
SORVALL
® Applications Laboratory.
The RC-5C PLUS allows you to obtain the integral value generated
during a normally timed run by pressing the OFF/
∫ω
2 dt switch to
∫ω
2 dt when the run has stopped (see Integral Recall). The integral
value can then be used to control subsequent centrifuge runs.
This procedure specifies how to set up a run to be controlled by an
integral value. Prepare the rotor according to the instructions in the
rotor instruction manual, then follow the instructions below.
NOTE
If desired, precool the rotor in the centrifuge according to
the procedure on page 4-7.
1. Follow Steps 1 through 9 and Step 12 in Normal Mode Opera-
tion to install a rotor, set run temperature and speed, and set
brake. To use the rate control, see Rate Control (ARC) Opera-
tion.
Follow all WARNINGS and CAUTIONS.
∫ω
2 dt = [(rpm/60)(2
π
)] 2 (t
2
-t
1
)
(t
2
-t
1
) = time in seconds
Figure 4-1. Integral Value
TIME
SPEED