Thermo Scientific Shake 'n Stack 6244, Руководство по эксплуатации и техническому обслуживанию

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© Thermo
Scientific
, May 2003. Issue 7 ii
CHAPTER 7: TROUBLESHOOTING GUIDE FOR NUCLEIC ACID
HYBRIDISATIONS USING THERMO OVENS ............................... 25
Background Reduction – General ................................................... 25
Prehybridisation Procedure ............................................................. 25
Washing Procedure......................................................................... 26
Probe Preparation ........................................................................... 26
Summary of Factors Resulting in High Backgrounds ...................... 27
Summary of Factors Resulting in a Signal Lower than Expected .... 28
APPENDIX I: SOLUTIONS FOR NUCLEIC ACID BLOTTING &
HYBRIDISATION
PROCEDURES.................................................. 29
APPENDIX II: FACTORS AFFECTING STRINGENCY OF HYBRIDISATION
REACTIONS ................................................................................... 32
Effect of Temperature & Salt Concentration.................................... 33
Summary of Conditions Affecting Hybridisation............................... 33
APPENDIX III: HYBRIDISATION BOTTLE SAFETY.............................................. 35
Use of the Bottle Gripper................................................................. 35
Bottle Care ...................................................................................... 35
APPENDIX IV: REFERENCES................................................................................ 36
HYBRIDISATION BOTTLE SAFETY: It is important to follow correct safety procedures when
using Hybridisation Bottles. Please see Appendix II for details