© Thermo
Scientific
, May 2003. Issue 7
22
11. Perform washes in bottle replacing each wash with: -
2 x 5 minutes
in 50-100ml 4 x SSC
1% SDS at 68
°
C
2 x 15 minutes in 50-100ml 2 x SSC
0.1% SDS at 68
°
C
1 x 15 minutes in 50-100ml 0.1 x
SSC
0.1% SDS at 68
°
C
Alternatively, remove membrane from roll and wash in plastic box: -
2 x 5 minutes
in 500ml 2 x SSC
0.1% SDS at room
temp.
2 x 15 minutes in 500ml 0.2 x SSC
0.1% SDS at 68
°
C
Содержание Shake 'n Stack 6244
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Страница 51: ...HYBRIDISATION GUIDE USER INSTRUCTION MANUAL Manual 7222060 Rev 0...
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