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Operating Instructions Biological Safety Cabinet | MSC-Advantage

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11

Disposal

11.1

Disposal procedure

Discarded cabinets or device components contain reusable materials. All com-
ponents with the exception of the HEPA filters can be discarded after having been
thoroughly cleaned and disinfected. The HEPA filters must be discarded in ac-
cordance with the applicable national and state regulations for special solid waste.

CAUTION – Contamination hazard!

As the device can be used for processing and
treating infectious substances, it may be
contaminated.
Prior to disposal, the complete device with
filters must be decontaminated by performing
a formaldehyde sterilization!

Recyclable materials!

Component

Material

Printed circuit boards

Enclosed electrical components coated with various
plastics, mounted on epoxy resin-bound boards.

Plastic components, general

Note material labeling

Exterior housing

Steel, painted

Device backpanel

Stainless steel/steel, painted

Front window

Multi-layer safety glass

Operating panel and indicator
foil

Polyethylene

Workplates

Stainless steel

Armrests

Stainless steel

Diffusor

Aluminium, anodized

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Содержание MSC-Advantage

Страница 1: ...Valid Operating Instructions Biological Safety Cabinet MSC Advantage Valid 04 2011 50128856 ...

Страница 2: ... Electron LED GmbH This regulation does not apply to reproductions for in plant use Trademarks MSCAdvantage and SmartFlow are registered trademarks of Thermo Scientific Thermo Scientific is a brand of Thermo Fisher Scientific Inc All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers Thermo Electron LED GmbH Robert Bosch Straße 1 D 6...

Страница 3: ...port 15 4 Description of the device 16 4 1 Overall view 16 4 2 Safety system 18 4 3 Filter system 19 4 4 Operating and display elements 20 4 5 Sample chamber access 21 4 6 Device interfaces 22 4 7 Sample chamber illumination 24 4 8 UV lamp unit 24 4 9 Working area 24 5 Start up 25 5 1 Initial operation 25 5 2 Installing the device and accessories 25 5 3 Unlocking the transport protection 26 5 4 Le...

Страница 4: ... Contents 8 Shut down 37 8 1 Interrupting an operation 37 8 2 Shutting the device down 37 9 Cleaning and decontamination 38 9 1 Decontamination procedure 38 9 2 Wipe spray disinfection 38 9 3 UV disinfection after a wipe spray disinfection 40 9 3 1 UV disinfection using the integral UV lamp 40 9 3 2 UV disinfection using a mobile UV device optional 40 9 3 3 Changing UV disinfection time 40 9 4 Dis...

Страница 5: ...114 EMail info labequipment de thermofisher com Enquiries from Europe Middle East and Africa Phone 49 0 6184 90 6940 Fax 49 0 6184 90 6772 EMail info labequipment de thermofisher com Postal address USA Thermo Fisher Scientific 275 Aiken Road Asheville NC 28804 USA Enquiries from North America Phone 1 800 879 7767 Fax 1 828 658 0363 EMail info labequipment de thermofisher com Enquiries from Latin A...

Страница 6: ...vent accidents The biological safety cabinet must be operated only by trained and authorized personnel For any operation of this device the operator must prepare clear and concise written instructions in the language of the operating and cleaning personnel ba sed on these operating instructions applicable safety data sheets plant hygiene guidelines and technical regulations in particular which dec...

Страница 7: ...ions and maintenance are performed at the specified intervals an installation test is performed prior to the initial operation of the device and that a repeat test is performed on the occasion of all inspections and repairs The warranty is valid from the date of delivery of the device to the operator 1 5 Standards and safety regulations The device complies with the safety requirements of the follo...

Страница 8: ...1 6 1 Symbols used in the operating instructions WARNING is used if non observance may cause serious or even lethal injuries CAUTION is used if non observance may cause medium to minor injuries or damage NOTE is used for hints and useful information RECYCLING Valuable raw materials can be reused Warning against electric shock ...

Страница 9: ...s switchbox ceiling Warning against hand injuries device side walls Biohazard left front section of device CE Conformity declaration Checked safety cover light dome Norme Française NF Postes de Sécurité Microbiologique 2 x T 5A T5A note sample chamber fusing RS 232 interface top side of plenum panel Installion of the armrests right side of the light hood ...

Страница 10: ...t be do cumented by a test report that shows that all operating parameters are in com pliance with those specified by Thermo Fisher Scientific 1 7 2 Incorrect use The biological safety cabinet must not be used in laboratories that do not comply with the requirements of safety levels 1 2 or 3 The device must not be operated as a Class II biological safety cabinet if no repeat test is performed afte...

Страница 11: ...ce has been delivered immediately check the device for completeness for possible damage If the delivery is incomplete or if you detect any transport damage to the device contact the forwarding agency and Thermo Fisher Scientific im mediately 2 3 Transport security lock and device packaging A transport security lock protects the device counterweight during transport Protective packagings protect th...

Страница 12: ...fficient load bearing capacity twice the device weight The room in which the device is installed must be of adequate height For devices that are not connected to an exhaust system the distance between the exhaust air opening at the device ceiling and the room ceiling must be at least 200 mm 8 in The location must be equipped with an appropriate ventilation system see Section 3 2 For the valves may...

Страница 13: ...ir flow is created in the installation space The volume of air discharged by the exhaust system must be tracked accordingly into the installation space It should be noted that the equipment air flow itself is not affected The on site exhaust system should be dimensioned so the air velocity scale in the draft interruptor and exhaust air plenum is sufficient that the equipment exhaust air has no air...

Страница 14: ...ant traffic and within the exhaust air range of a ventilation system 4 Preferred locations The locations 6 7 and 8 are correct because they are in a draft free section of the room and not ex posed to plant traffic A counterweight at the device backpanel moves synchronously with the vertical movement of the front window To prevent the counterweight from jamming the device backpanel should be as clo...

Страница 15: ...uding inside buildings use an appropriate lifting device which ensures that the unit is on a stable stand and is secured against lateral tilting Do not transport the biological safety cabinet on a base with roles CAUTION Lift points For transport lift the device only at the lift points shown in Figure 2 Do not allow the weight of the cabinet to rest on the floorpan CAUTION Crushing hazard The weig...

Страница 16: ... 0 9 1 5 1 8 The optional device integral UV lamp is installed to the ceiling at the front section of the sample chamber Operating panel 5 with function keys and indicators Front window 7 with two handles 6 Bushings 8 and 9 in the side panels 3 on each side The bushings 9 can be used to install media valves 11 the bushings 8 of type SmartPort are provided for laying cables or hoses into the utilit...

Страница 17: ...17 Operating Instructions Biological Safety Cabinet MSC Advantage 50128856_04 2011 4 Description of the device Fig 3 Overall view ...

Страница 18: ...tection from cross contamination HEPA filters The downflow i e the air circulating within the device and the exhaust air air that is released to the exterior are cleaned by HEPA filters HEPA High Efficiency Particulate Air Filter Safety lockout To protect from UV radiation the optional UV disinfection routine can be run only if the front opening is closed During UV disinfection the front opening s...

Страница 19: ...ample cham ber through the working opening In the air duct room air and the downflow within the chamber 7 are then blended to make up the blend air 8 The blend air is then filtered proportionally by the downflow filter 5 and supp lied as ultrapure air 6 evenly into the sample chamber of the device filtered by the exhaust air filter 2 and released as ultrapure air 1 to the exterior of the device In...

Страница 20: ...wing information re gardless of the activated operating function normal operation operating hours of the device safe work mode downflow and exhaust air velocities calibration routine status of the calibration function device controlled UV disinfection optional remaining disinfection time Values are output as integers B Keys for switching operational functions on or off C Status indicators show the...

Страница 21: ...E Status indicator The two positions A and C are determined by switches and output as status indicators on the display see Section 6 1 1 For cleaning maintenance SmartClean window cleaning position with opening height D for cleaning and disinfecting the upper section of the front win dow For this purpose the front window can be lowered beyond closed position C so that a sufficiently high gap exist...

Страница 22: ... 9 1 2 4 outlets model MSC 1 5 1 8 Optionally an outlet can be replaced with a disinfection adapter 9 for the con nection of a mobile UV device Two fuse holders for 5 A miniature fuses at the top of the front cover protect the device integral power supply 1 for L 2 for N Communication port The top of the device side contains an RS232 connec tion 3 for a PC Bushings The standard fittings are 3 bush...

Страница 23: ...tial equalization To avoid static charges and the associated risks if necessary intended supply connections and the device itself should be integrated in the on site potential equalization For this purpose the potential equalization connection on the stand can be used The installation of utility connections to the built in fittings must be made with con sideration to the current national technical...

Страница 24: ...closed When the front window is opened the UV di sinfection routine is cancelled immediately 4 9 Working area The standard equipment comprises the segmented work plate The workplate segments are placed onto the frame above the sample chamber floorpan NOTE Workplate condition Use properly plane lying workplates only Replace deformed workplates Pay attention to the maximum work load see sction 12 Fi...

Страница 25: ...vice without stand onto a sufficiently stable substructure so that the weight of the device frame does not rest upon the floorpan Remove the protective foil from the floorpan Device with stand Assemble the stand accessory and install the device frame onto the stand 1 Fig 10 Slide the two crossmembers 2 onto the retaining angles 3 of the side panels 1 then secure the crossmem bers to the side panel...

Страница 26: ...ht To unlock the transport protection Remove the four retaining screws position see arrows Keep the counterweight retaining screws for later transports 5 4 Levelling the cabinet The cabinet should be levelled only after it has been positioned 1 Remove transport protection vinyl from the workplate or from the workplate 2 Place the workplate onto the front and rear support rails in the sample chambe...

Страница 27: ...hesive bases and cable ties see installation instructions 4 Make sure that the power supply line is not subjected to tensile or compressive force Installation of the power supply connection To protect from accidental switch off the power supply outlets should be loca ted out of casual reach und must be accessible only to authorized persons Ideally the outlet should be located above the biological ...

Страница 28: ... If the airflow is disturbed during this run time the process is cancelled and the calibration routine is restar ted To complete the routine the determined parameters for safe operation and the alarm limits are stored automatically NOTE Initial operation According to applicable national standards and regulations the calibration routine is no substitute for a start up performed by an au thorized se...

Страница 29: ...repairs to the device or after consi derable changes more than 5 cm to the location of the device The operator must prepare a test report or request a written test report from the authorized test service NOTE Safety warranty The operational safety of the device particu larly the personal and material protection are guaranteed only if all safety functions of the device have been tested and approved...

Страница 30: ...dual function Indicating operating data Press the key to switch between the data Operating hours downflow and exhaust air velocities in the safe work mode Enabling I disabling 0 the alarm acknowledge function In the OFF mode keep the key depressed for approx 5 second The display momentarily shows the operating hours then the switching state I alarm acknowledge function enabled 0 alarm acknowledge ...

Страница 31: ...een 17 green sufficient compensation capability 16 yellow 15 yellow compensation capability is depleted 14 red 13 red window outside working position or air velocity outside alarm range NOTE Compensation capability If the display is steady yellow yellow there is a possibility that the filters of the device are fully loaded In this case the technical service should be informed 6 1 2 Display during ...

Страница 32: ...t audible blower operation the status indicators LEDs 9 12 illuminate NOTE Switch on A time delay of several seconds may occur between key actuation and device response 2 Move the front window to the work position The correct position is reached when the green status indicator FRONT WINDOW IS IN WORK POSITION 12 is illu minated The side guide rails have markings that define the lower edge of the f...

Страница 33: ...DOW NOT IN WORK POSITION is illumina ted The sample chamber illumination is enabled The internal power supply is enabled If the internal power supply is activated the status indicator INTERNAL POWER SUPPLY ON is illuminated Work mode Ensures personal and material protection In this operating sta te the work process is carried out in the sample chamber Work mode is acti ve when the front window is ...

Страница 34: ...low status indicator INTERNAL POWER SUPPLY ACTIVATED is illuminated The biological safety cabinet can be switched off in OFF mode Keep the ON key depressed until all indicators are off UV mode For running the UV disinfection routine the front window is completely lowered to protect against UV radiation The routine cannot be run until the front window is in the closed position The UV disinfection r...

Страница 35: ...orking properly If the alarm system issues failure messages when the front window is in the work position stop all applications that may release harmful aerosols 4 Load the work surface with samples 5 For work intermissions or for extended experimental phases without manual in tervention switch the device to standby mode 7 3 Response to failure messages Failure messages are displayed in form of te...

Страница 36: ...excessive heat Do not block air circulation at the ventilation slots of the workplate Sitting posture during work Fig 15 To prevent risks to health a height adjustable working chair with an adjustable seat back should be used during extended work periods at the biological safety cabinet A When the forearm rests on the armrest is should be in a nearly horizontal position B When the upper leg is in ...

Страница 37: ...til the indicators are off the right indicator segment of the display shows a dot to indicate that voltage is present NOTE Blower switch off For safety reasons the blowers can be swit ched off only when the front window is closed 8 2 Shutting the device down If the device is not to be used or stored for an extended period of time it must be completely decontaminated WARNING Decontamination measure...

Страница 38: ...ts and the protective grid see Section 9 6 9 7 Disinfection with formaldehyde can be performed if a sterile sample chamber is required for the work process This sterilization procedure is mandatory when filters are replaced when the device is shut down when the device is discarded 9 2 Wipe spray disinfection The wipe spray disinfection is performed in three stages predisinfection cleaning final di...

Страница 39: ...ckets wipe them thorou ghly using a damp cloth 6 Switch the device to work mode move the front window to the work position 7 Allow disinfectant to react as recommended by the manufacturer then operate the biological safety cabinet for at least 15 to 20 minutes in the work mode so that released aerosols can be absorbed by the filters Cleaning 1 Remove dirt residues and deposits thoroughly using a s...

Страница 40: ...ible with the UV disinfection adapter of the biological safety cabinet To start the UV disinfection routine 1 Position the mobile UV device centered on the work area and connect it to the UV disinfection adapter 2 Close the front window completely the air system operates in the reduced mode yellow LED illuminated 3 Start UV disinfection using the UV key at the operating panel Keep the UV depressed...

Страница 41: ...cessories The temperature at the cabinet location should be approx 21 C the relative humidity should be between 60 and 85 To evaporate the solution a heating device with a container is required NOTE Disinfection procedure A disinfection with formaldehyde must be performed in accordance with the specificati ons of EN 12469 As this procedure has considerable risks it must only be performed by specia...

Страница 42: ...moved completely from the floorpan 5 Reinstall the workplates 9 8 Cleaning the protective grid Fig 16 The multi segment protective grid is retained in the installed position between support surface and airduct wall by its own tension 1 To remove a grid segment 2 push the retaining tab 1 down until the grid segment can be removed from below the counterholder 3 of the wall 2 To install the grid segm...

Страница 43: ...f the device Checking all components for possible damage Checking the filter state NOTE Perforated plate The perforated plate at the sample chamber ceiling serves as the protection of the down flow filter and prevents refluxing While scanning the filter surface for a leak test the perforated plate must be installed Checking the airflow conditions Repeat test in accordance with EN 12469 2000 10 2 S...

Страница 44: ...nt win dow The UV lamp should be replaced after 1500 operating hours 1 Switch the device off disconnect it from the power supp ly system and protect it against accidental reconnection 2 Move the front window to the maximum opening position 3 Wear protective gloves to prevent skin fat residues from burning into the lamp tube The UV lamp is installed in rotating sockets To remove ro tate the lamp co...

Страница 45: ...e removed inwards into the utility chamber 1 First remove the inner grommet of the SmartPort in the utility chamber inwards from the side panel opening 2 Then remove the outer grommet of the SmartPort in the utility chamber inwards from the side panel opening 3 Disinfect both grommets in the utility chamber and take them out of the utility chamber 4 Insert new outer grommet in the side panel openi...

Страница 46: ...s the device can be used for processing and treating infectious substances it may be contaminated Prior to disposal the complete device with filters must be decontaminated by performing a formaldehyde sterilization Recyclable materials Component Material Printed circuit boards Enclosed electrical components coated with various plastics mounted on epoxy resin bound boards Plastic components general...

Страница 47: ...e following symbol Thermo Fisher Scientific has contracted with one or more recycling disposal com panies in each EU Member State and this product should be discarded or re cycled through them For further information on Thermo Scientific s compliance with these Directives contact the recyclers in your country and information on Thermo Scientific products wich may assist the detection of substances...

Страница 48: ...48 Operating Instructions Biological Safety Cabinet MSC Advantage 50128856_04 2011 12 Technical data ...

Страница 49: ...eration C Min ambient temperature during operation C Max humidity operation r H Max gumidity storage r H Room temperature 20 C kJ s Ergonomics Ambient conditions The noise level was determined in accordance with EN ISO3744 The sound pressure level was measured at a distance of 1 m in front of the working opening The measurement uncertainty is within a range of 2 dB 40 10 Temperature Humidity 90 no...

Страница 50: ...t fusing A On site fusing A Power input max W Protection class Protection type Overvoltage category IEC 1010 EN 61010 Contamination degree IEC 1010 EN 61010 Mains connection 3 5 2 2 x T 5 A Circuit breaker B16 Fuse T 16 A Power 1700 0 2000 0 2 x T 16 A Current 7 3 8 7 Electrical data Voltage 1 N PE AC 230 V 50 60 Hz 48 V DC MSC 1 5 MSC 1 8 MSC Advantage MSC 0 9 MSC 1 2 Connecting lines Cable 3 7 m...

Страница 51: ...eparability in MPPS Width mm Depth mm Height mm Width mm Depth mm Height mm Airflow system Airflows Airflow velocities 0 45 0 32 MSC 1 5 MSC 1 8 Description MSC 0 9 MSC 1 2 457 117 610 915 610 610 Separability at 0 3 µm particle size Exhaust air 457 94 Filters 1525 1830 99 995 99 999 Downflow 915 1220 HEPA H 14 according to DIN EN 1822 Glas fiber fleece 1523 1827 1037 1244 622 389 486 583 Air volu...

Страница 52: ...erating Instructions Biological Safety Cabinet MSC Advantage 50128856_04 2011 13 Device log Part number Serial number Service number Location Operator s note Date Signature Device type Work carried out Notes ...

Страница 53: ...53 Operating Instructions Biological Safety Cabinet MSC Advantage 50128856_04 2011 14 Certificate of decontamination ...

Страница 54: ...54 Operating Instructions Biological Safety Cabinet MSC Advantage 50128856_04 2011 Internet http www thermofisher com ...

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