Functional Description
Principle of magnetic particle processing
Thermo Fisher Scientific
Thermo Scientific KingFisher Duo User Manual
15
without interruptions. When using large-volume configurations on 24-
well plates, usually two plates are used in a run.
Before starting the magnetic particle processing via the keypad and
display, the samples and reagents are dispensed into the plate(s)/strip(s)
and the tip comb is placed onto the preset row of the sample plate,
default row B, from which it is automatically loaded. The plate(s) are
placed onto the turntable into the corresponding plate position
according to the protocol instructions. During the operation, the front
lid can be closed or open (Figure 2–3). The closed door protects the
processing against environmental contamination.
The operating principle employed is MPP (inverse magnetic particle
processing) technology (Figure 2–7). Rather than moving the liquids,
the magnetic particles are moved row wise on the plate(s)/strip(s)
containing specific reagents, in contrast to the external magnet method.
Magnetic particles are transferred with the aid of magnetic rods covered
with a disposable, specially designed plastic tip comb.
Figure 2–6.
KingFisher Duo magnetic particle processor
Figure 2–7.
Inverse magnetic particle processing
Principle of magnetic
particle processing
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