Thermo Scientific KingFisher Duo, Руководство пользователя

Страница: 55 / 84

Routine Operation
How to start
Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual 53
Caution Only use plates that are listed in Table 4–4. Other plates may
not be compatible with the KingFisher Duo heating blocks. They may
also cause unexpected problems, such as cross-contamination due to the
divergent well volume and bottom height of the plate.
▲
Table 4–4. Processing volumes vs. plate types and magnet heads
Head / Plate KingFisher Flex 24 deep
well plate Microtiter deep well
96 plate KingFisher Duo
elution strip
KingFisher Duo head for
24 DW plate 200–5000 μl – –
KingFisher Duo head for
96 DW plate
– 50–1000 μl 30–130 μl
The KingFisher Duo elution strip can only be used with a KingFisher
Duo instrument installed with a 12-pin magnet head (Figure 4–28).
Figure 4–28. KingFisher Duo elution strips, separate and in a frame
The elution strip is typically used to perform the elution step that
concentrates the purified sample. It is easy to pipette small volumes into
the elution strip due to its low design. The eight elution strips are also
convenient for storage and can be collected onto the 96 elution frame
(Figure 4–28).
To start the instrument:
1. Select a protocol by using the arrow keys and press
START
or use
BindIt Software to run the desired protocol via the PC.
2. Ensure that the front lid is open (Figure 2–3).
3. Load the plate(s) and elution strip(s) in the order that the protocol
requests (Figure 4–30). Place the A1 well of the plate ensuring that
it is in the upper right corner. The first A1 row is consequently
always in the inner circle. Once you have loaded the requested
plates into their respective plate positions, press
OK
. The instrument
Elution strip
How to start