Thermo Fisher Scientifi c
Thermo Scientifi c Novus SCP/MCP User Manual
17
CALIBRATION
All Finnpipettes are factory calibrated and adjusted to give the volumes as
specifi ed with distilled or deionized water. Normally, the pipettes do not need
adjustment, but they are constructed to permit recalibration and adjustment for
liquids of different temperature and viscosity.
An analytical balance must be used. The scale graduation value of the balance
should be chosen according to the selected test volume of the pipette:
Volume readable
Precision Uncertainty
of
range graduation
repeatability(s)
measurement
and
linearity
under 10 μl
0.00 1mg
0.001mg
0.002μl
10-100 μl
0.01 mg
0.02mg
0.02μl
above 100 μl
0.1 mg
0.2mg
0.2μl
If the uncertainty of measurement of the balance is known, this may be used
instead of the repeatability and linearity.
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696.
Tests are done in a draft-free room at a constant (±0.5°C) temperature of water,
pipette and air between 15°C to 30°C.
The relative humidity must be above 50%. Especially with volumes under 50μl
the air humidity should be as high as possible to reduce the effect of evaporation
loss. Special accessories, such as the evaporation trap, are recommended.
By selecting MENU -> OPTIONS -> CALIBRATE -> COUNTER the number of
pipetttings since last calibration is shown on the display. The counter is reset
to zero when calibration is performed.
The pipette is checked with the maximum volume (nominal volume) and with
the minimum volume. A new tip is fi rst pre-wetted 3-5 times and a series of
ten pipettings are done with both volumes. A pipette is always adjusted for
delivery (Ex) of the selected volume. Measuring volumes taken from balance
is not allowed.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the
pipette is correct.
Device
requirements and
test conditions
Calibration
Counter
Checking the
calibration
