14
Thermo Scientifi c Novus SCP/MCP User Manual
Thermo Fisher Scientifi c
Seq stepper
The sequential stepper mode enables serial dispensing of different volumes (in
normal stepper mode only fi xed volume). First choose the amount of dispensings
(max. 20) with SCROLLKEY and accept with OK. Vol 1 appears to display and
highest possible volume is fl ashing. Select the fi rst volume with SCROLLKEY
and accept with OK. Now Vol 2 appears to display and highest possible volume
left is fl ashing. Select the second volume with SCROLLKEY and accept with
OK. After selecting the last volume the total volume is shown on display and
speed in is fl ashing. Select the pipettings speeds and the pipette is ready for
pipetting.
1. Dip the tip under the surface of the liquid in the reservoir and press the
trigger. This action will fi ll the tip and the fi rst volume appears on the display.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the fi rst volume by pressing the trigger. The display shows the next
volume.
4. Continue dispensing by repeating step 3. After last step a text BLOWOUT is
displayed.
5. To empty the tip completely, press trigger.
6. If necessary, change the tip and continue pipetting.
Note
The pipette can be emptied at any time by pressing CANCEL (left selection
key)
Mix + Dilute
Select the fi rst pipetting volume by pressing the scroll key up or down. Accept
the volume with OK. Next select the second volume (VOL 2) with scroll key and
accept with OK. Then select the pipetting speeds.
1. Dip the tip under the surface of the fi rst liquid in the reservoir and press the
trigger. The fi rst volume is drawn in to the tip. A text AIR appears on display.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir
to remove excess liquid. Press trigger again to aspirate air buffer.
3. Dip the tip under the surface of the second liquid and press the trigger. The
second volume is drawn in to the tip.
4. Withdraw the tip from the liquid.
5. To deliver the both volumes, press the trigger. A text MIX appears on the
display.
6. By pressing trigger the pipette starts to pipette ca. 70 % of the total volume
several times as long as the trigger is held pressed.
7. After releasing the trigger the pipette stops after next dispensing and a text
BLOWOUT appears to display.
8. Withdraw the tip from the liquid and press the trigger and hold it down to
empty the tip.
9. Release the trigger to return to the ready position.
10. If necessary, change the tip and continue pipetting.
