background image

8

Operational Procedure 

8. Operational Procedure 

The ALPS 3000 has been designed to reliably seal plates of different heights and different plastics, using a 
variety of films. These components will require different sealing conditions.

The quality and strength of the seal created between the sealing film will vary with different conditions. In 
general, increasing either the sealing temperature or duration of seal, gives a stronger, more complete seal; 
however over-sealing on a regular basis can cause damage to the plate being sealed. This in turn, reduces 
the number of times a particular plate can be resealed. Therefore a balance has to be achieved that gives 
an acceptable seal with the minimal plate damage or distortion. 

Another optimization factor to be taken into account is the surface area of the plate.

Plate with thin raised rims around each tube, have a reduced surface area compared to a plate with wide 
raised rims. Therefore less heat is needed to seal the thin rim plate in comparison to the wide rim.

The pressure that the heater plate exerts during sealing is pre-set and cannot be adjusted. Plates without 
raised seals are not considered suitable for heat sealing. 

8.1 - Temperature and Time Setting:

The following table gives a guide as to the sealing time and temperature for different materials.

 

Plate Material  

Temperature Setting  

Time Setting

 

Polystyrene  

145° C - 165° C  

3 Seconds - 5 Seconds 

 

Polypropylene  

150° C - 175° C  

3 Seconds - 5 Seconds 

 

Polyethylene  

150° C - 170° C  

3 Seconds - 5 Seconds

8.2 - Commands, Indications and warnings:

The ALPS 3000 is software controlled, employing micro-controllers. All commands, indications and 
warnings are effected via the touch sensitive LCD display panel located on the front of the unit.

The sequence of events is as follows:

8.3 - Setting the sealing operation:

With a plate in position on the Shuttle, the SEAL segment is pressed to start the sealing operation.

If the sealing plate is not up to temperature, the display will indicate it is heating, and the actual 
temperature compared to the set temperature. The display flashes ‘Sealing’ during the operation and then 
returns to the seal screen, ready for another operation.

 

Содержание ALPS 3000

Страница 1: ...ThermoScientificALPS3000 AutomatedLaboratory PlateSealer UserManual...

Страница 2: ...ries No part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means in whole or in part without the prior written permission of Thermo Fisher Sci...

Страница 3: ...ent Footprint 4 5 Connecting the Air Supply 5 6 Connecting the Voltage Supply 6 7 Foil Film Loading 7 8 Operational Procedure and Optimization 8 9 Communication Protocol 10 10 Cleaning the ALPS 3000 1...

Страница 4: ...ion companies Procedure The unsealed plate is placed on a shuttle which extends from the front of the sealer allowing full access with a robotic arm or stacking system The shuttle is drawn into the un...

Страница 5: ...s will the supplier of this equipment be liable for any incidental consequential or any special damages of any kind whatsoever including but not limited to lost profits arising from or in anyway conne...

Страница 6: ...uld be switched on ten minutes prior to use to enable the set temperature to be reached The unit should only be used in a suitably ventilated area The use of solvents on the unit is not recommended Ce...

Страница 7: ...llow 170mm at the front of the unit for the plate carrier to open and 100mm at the back and top for the seal roll The ALPS 3000 should be operated in an environment with a temperature range of 15o C 4...

Страница 8: ...an air connector to the ALPS 3000 always use 2 spanners one to tighten the connector and one to prevent the bulkhead fitting from turning If the bulkhead fitting is allowed to rotate it may become loo...

Страница 9: ...d and switched Plug the IEC Lead in the inlet and switch on the Rocker I O Switch The display that is mounted on the front of the unit should illuminate NOTE The system is self regulated to sense volt...

Страница 10: ...l through the foil gripper then through the cutting section and finally bring the tool out to the rear of the machine this is best carried out by placing the foil loading tool flat on the plate shuttl...

Страница 11: ...plate in comparison to the wide rim The pressure that the heater plate exerts during sealing is pre set and cannot be adjusted Plates without raised seals are not considered suitable for heat sealing...

Страница 12: ...foil Whilst holding surplus foil pulled through with foil loader centre foil on shuttle and Press Trim Whilst trimming pull gently on foil until fully removed from machine 8 7 To set Remote Mode Pres...

Страница 13: ...Pin 2 Pin 3 Pin 3 Pin 5 Pin 5 9 3 Message Response time The unit responds to each message within 100 msec 9 4 Configuring for remote operation On the touch screen use the advanced menu to set the fol...

Страница 14: ...e responses to the status message enquiry are hexadecimal values which when converted to binary values allow the status to be interpreted For example when the unit is heating or cooling and so not at...

Страница 15: ...ure message PC sends A cr ALPS 3000 replies ok cr Where is a 3 digit decimal number represented in ASCII i e the string A180 cr sets the sealing temperature to 180 C 9 14 Sealing Time message PC sends...

Страница 16: ...number represented in ASCII in units of 10ths of a second i e the string 25 cr means the current sealing time is 2 5 seconds 9 17 To read back sealing temperature actual value PC sends F cr ALPS 3000...

Страница 17: ...n that recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the unit Always remove the power and pneumatic supplies before cleaning the uni...

Страница 18: ...m W x H x D Weight 12Kg Air Input 1 4 BSP Female Thread Air Requirement 50 Litres per minute Air Input Pressure 5 5 Bar 80 PSI Minimum Operating Pressure 5 5 Bar 80 PSI Minimum Operating Ambient Tempe...

Страница 19: ...n instrument integrity and optimal performance Should a problem occur with the sealing an initial inspection may identify its cause and the necessary corrective action The following list highlights po...

Страница 20: ...volume If no improvement call for service Fails to respond to an input Check air as above Doesn t cut properly Tool setting problem call for service Foil not picked up Check display for vacuum error C...

Страница 21: ...or sample retrieval AB 3739 Easy Peel PP PE COC 1 5 2 5 seconds at 165 170 C 200 C to 90 C and up to 120 C with cycler pressure Description Foil laminate difficult to pierce peelable bond that can be...

Страница 22: ...Thermal bath water in use Including PCR as such applications temperature high as well as temperature low and transportation including storage term long for microplates polypropolene sealing for ideal...

Страница 23: ......

Страница 24: ...cientific Inc and its subsidiaries NorthAmerica Tel 800 345 0206 Europe Tel 44 0 161486 2110 web www thermoscientificcom Sample Storage and Microplates Choose from a wide variety of sample storage sol...

Отзывы: