3
– Operation
Thermo Scientific
Product Manual for Dionex Anion Chemically Regenerated
Suppressor for ICE
Page 16 of 22
032661-12
3.3
Determining Eluent and Regenerant Concentrations
A.
Unlike packed bed suppressor columns, which entail periodic shutdowns for
regeneration, the Dionex ACRS-ICE 500 has the ability to provide continuous
suppression.
B.
The operation of the Dionex ACRS-ICE 500 requires a constant flow of the regenerant
over the membrane, in a direction that is countercurrent to the flow of the eluent. A
standard regenerant flow rate of 2–3 mL/min is recommended. This flow rate is
adequate for most applications. For applications that require stronger than typical
eluent strengths, the regenerant flow rate may be increased up to a maximum of 5
mL/min.
C.
The Dionex ACRS-ICE 500 is compatible with acetonitrile, methanol, ethanol, and
isopropanol. The maximum solvent level is 90%. For more information about solvent
compatibility, call the Thermo Scientific Dionex Technical Services Hotline.
Do not use THF solvent in the eluent.
D.
5 mM tetrabutylammonium hydroxide (TBAOH) is the recommended regenerant for
use with the Dionex ACRS-ICE 500. Tetramethylammonium hydroxide or potassium
hydroxide may be used as alternate regenerants, but cause higher background
conductivity and therefore compromise total system performance. For ease of
preparation and guaranteed purity, use Dionex Cation Regenerant Solution (P/N
039602).
E.
For the best signal to noise ratio and overall performance, Dionex recommends that the
Cation Regenerant Solution (P/N 039602) be pressurized with nitrogen or helium. Do
not use air.
Use nitrogen or helium to pressurize the Regenerant Reservoir.
3.4
Dionex ACRS-ICE 500 Storage
The Dionex ACRS-ICE 500 is shipped with an acidic eluent and TBAOH regenerant as the
storage solution. The suppressor’s storage solution should be the eluent acid
(Heptafluorobutyric acid or HCl) at the same concentration used in the application without
solvent. If the suppressor will not be used for more than one week, prepare it for storage. The
screens and membranes in the Dionex ACRS-ICE 500 must be completely hydrated to maintain
liquid seal and chromatographic performance.
NOTE
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NOTE
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