3
– Operation
Thermo Scientific
Product Manual for Dionex Anion Chemically Regenerated
Suppressor for ICE
Page 14 of 22
032661-12
3.
Operation
This section provides instructions for the start up and operation of the Dionex Anion Chemically
Regenerated Suppressor for ICE (Dionex ACRS-ICE 500).
3.1
Chemical Purity Requirements
Obtaining precise and accurate results requires eluents that are free of ionic impurities.
Chemicals and deionized water used to prepare eluents must be of the purities described below.
Low trace impurities and low particulate levels in eluents and regenerants also help protect your
Dionex ACRS-ICE 500 and system components from contamination. Thermo Fisher Scientific
cannot guarantee proper Dionex ACRS-ICE 500 performance when the quality of the chemicals
and water used to prepare eluents has been compromised.
3.1.1
Inorganic Chemicals
Reagent Grade inorganic chemicals should always be used to prepare ionic eluents. Whenever
possible, inorganic chemicals that meet or surpass the latest American Chemical Society
standard for purity (universally accepted standard for reagents) should be used. These inorganic
chemicals will detail the purity by having an actual lot analysis on each label.
3.1.2
Solvents
Since solvents used with the Dionex ACRS-ICE 500 are added to ionic eluents to modify the
ion exchange process or improve sample solubility, the solvents used must be free of ionic
impurities. However, since most manufacturers of solvents do not test for ionic impurities, it is
important that the highest grade of solvents available be used. Currently, several manufacturers
are making ultrahigh purity solvents that are compatible for HPLC and spectrophotometric
applications. These ultrahigh purity solvents will usually ensure that your chromatography is not
affected by ionic impurities in the solvent. At Thermo Fisher Scientific, we have obtained
consistent results using High Purity Solvents manufactured by Burdick and Jackson and
Optima
®
Solvents by Thermo Fisher Scientific.
3.1.3
Deionized Water
The deionized water used to prepare eluents should be degassed Type I Reagent Grade Water
with a specific resistance of 18.2 megohm-cm. The deionized water should be free of ionized
impurities, organics, microorganisms and particulate matter larger than 0.2 µm. It is good
practice to filter eluents through a 0.2 µm filter whenever possible. Bottled HPLC-Grade Water
should not be used since most bottled water contains an unacceptable level of ionic impurities.
Finally, thoroughly degas all deionized water prior to preparing any eluents or regenerants.
3.1.4
Regenerant
For ease of regenerant preparation and guaranteed purity, use Thermo Scientific Dionex Cation
Regenerant Solution, 0.1 N tetrabutylammonium hydroxide (TBAOH), P/N 039602.
Tetrabutylammonium hydroxide (TBAOH) is the recommended regenerant for use with the
Dionex ACRS-ICE 500.
