Teledyne 340CF ELSD Скачать руководство пользователя страница 7 | Manualshive

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Teledyne 340CF ELSD Скачать руководство пользователя страница 7

vii

340CF ELSD

Table of Contents

Section 1 Quick Start Guide

1.1 Connecting to the CombiFlash® Rf200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Connecting to the CombiFlash® Torrent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
1.3 Nebulization Chamber Drain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
1.4 Basic Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Section 2 Introduction

2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3 Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

2.3.1 Nebulization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.3.2 Evaporation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.3.3 Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

2.4 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

Section 3 Installation

3.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

3.2.1 Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2.2 Exhaust . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2.3 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2.4 Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2.5 Digital I/O Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.6 Gas Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.7 Fluid Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.8 Nebulization Chamber Drain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.3 Connection to Teledyne Isco Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

3.3.1 Connecting to the CombiFlash® Rf200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.3.2 Connecting to the CombiFlash® Rf200 with 4X Module . . . . . . . . . . . . . . . 3-5
3.3.3 Connecting to the CombiFlash® Torrent Module . . . . . . . . . . . . . . . . . . . . 3-6

3.4 Conversion for HPLC Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

3.4.1 Operating Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3.4.2 Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9

3.5 Installation Qualification Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10

Section 4 Operation

4.1 Instrument Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

4.1.1 Status Indicator Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.2 Status Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.3 MENU|POWER Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.4 Autozero Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.1.5 Display Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

4.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9

4.2.1 Start Up Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.2.2 Choosing Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9

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Содержание 340CF ELSD

Страница 1: ...340CF ELSD Installation and Operation Guide Part 69 5243 095 Copyright 2006 Revision G July 25 2016 ...

Страница 2: ......

Страница 3: ... radioactifs Si vous devez utiliser tels liquides il est très recommandé que vous le faites à l intérieur d un environnement isolé conçu pour tels liquides Cet environnement isolé devrait être construit selon les règlements fédéraux provinciaux et locaux aussi que le plan de votre compagnie qui concerne l évènement d un accident avec les matières hasardeuses WARNING Avoid hazardous practices If yo...

Страница 4: ...i concerne les mésaventures de l hygiène ou de chimique En tout cas utilisez toujours de pratiques bonnes de la laboratoire et des procédures standardes de la sûreté Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts These three levels are described in the sample alerts below CAUTION Cautions identify a potential hazard which if not avoided may result in minor o...

Страница 5: ...ité Ce symbole signale l existence d instructions importantes relatives au pro duit dans ce manuel Ce symbole signale la présence d un danger d électocution Warnungen und Vorsichtshinweise Das Ausrufezeichen in Dreieck ist ein Warnzeichen das Sie darauf aufmerksam macht daß wichtige Anleitungen zu diesem Handbuch gehören Der gepfeilte Blitz im Dreieck ist ein Warnzeichen das Sei vor gefährlichen S...

Страница 6: ...ELSD Safety vi ...

Страница 7: ...l I O Connections 3 2 3 2 6 Gas Connections 3 2 3 2 7 Fluid Connections 3 2 3 2 8 Nebulization Chamber Drain 3 2 3 3 Connection to Teledyne Isco Instruments 3 3 3 3 1 Connecting to the CombiFlash Rf200 3 3 3 3 2 Connecting to the CombiFlash Rf200 with 4X Module 3 5 3 3 3 Connecting to the CombiFlash Torrent Module 3 6 3 4 Conversion for HPLC Applications 3 6 3 4 1 Operating Temperatures 3 9 3 4 2 ...

Страница 8: ... 2 2 2 3 Evaporation and detection 2 3 3 1 Attach drain adaptor prime with mobile phase if desired 3 2 3 2 Preparing the Rf200 connection ports 3 3 3 3 Connecting the Rf ports to the ELSD 3 3 3 4 Connecting gas supply to ELSD 3 4 3 5 Rf external detector cable connections 3 4 3 6 Component arrangement for connection with Rf200 and 4X module 3 5 3 7 Coupler for connecting 4X tubing 3 5 3 8 Case top...

Страница 9: ...technology to automate setting the parameters for purifi cation runs and fraction collection To connect the ELSD to the Rf200 1 On the Rf disconnect the tubing from ports C and D Retain the tubing assembly in case it is needed again later Figure 1 1 Preparing the Rf200 connection ports 2 Connect the shorter tubing assembly to port C and the lon ger tubing assembly to port D refer to Figure 1 2 3 C...

Страница 10: ...tting on the lower left rear panel of the ELSD with 1 8 O D tubing Figure 1 3 Connecting gas supply to ELSD 5 Connect the external detector cable Isco part 250 0000 20 to the Rf Figure 1 4 6 Connect the other end of the cable to the ELSD taking care to observe polarity as indicated by the small tab on the ground side of the connector see Figure 1 4 183cm 60cm ...

Страница 11: ...340CF ELSD Section 1 Quick Start Guide 1 3 Figure 1 4 Rf external detector cable connections Ground CU Ground Rf200 Connection ELSD Connection ...

Страница 12: ... PTFE tubing w nut and ferrule swaged one end and a union on the other end To connect the ELSD to the Torrent 1 With a 3 32 Allen wrench remove the PEEK tee fitting and metal tubing from the rear panel of the ELSD unit refer to Figure 1 5 2 Using the two long screws with lock and flat washers mount the metal tee fitting on the rear panel aligning the two standoffs over the ELSD s threaded mounting...

Страница 13: ...o the Torrent bottom column connection Connect the other end to the metal tee fitting on the back of the ELSD 6 Locate the other piece of black PTFE tubing from the kit Connect the metal union to the swaged tubing on the front of the Torrent Connect the swaged end of the tubing to the metal tee fitting on the back of the ELSD Figure 1 7 Tee fitting for Torrent to ELSD Disconnect Union ...

Страница 14: ...ith liquid is very important to ensure detection sensitivity Introduce 10 mL of mobile phase into the drain using a squirt bottle or syringe Figure 1 8 Attach drain adaptor prime with mobile phase if desired 1 4 Basic Operation Startup 1 Press MENU POWER to turn on the ELSD 2 Following power up diagnostics press MENU POWER to begin warm up 3 Turn on the gas supply to 65 5 psi Wait for the asterisk...

Страница 15: ...ent front peaks in the void volume with UV and RI detectors so early eluting analytes can be easily quantified Mass spectrometry MS detection is also a universal detector but its high cost and complexity have kept it from being widely used In fact the oper ation requirements of MS closely match that of the ELSD This allows the less expensive and complicated ELSD to be used as a method development ...

Страница 16: ...ture controlled walls When the aerosol exiting the nebulizer encounters a cool environment it partially condenses into larger particles whose momentum carries them into the wall and down the drain With cooling the particles con dense and increase in size They are carried into the walls of the bend and exit via the drain Figure 2 1 Thermo Split chamber Cooling With heat the particles decrease in si...

Страница 17: ...evaporation of the analyte or when working with thermally sensitive compounds Figure 2 3 Evaporation and detection 2 3 3 Detection The particles emerging from the evaporation tube enter the optical cell where the sample particles pass through the light emitted by a low power laser The particles scatter the light which is detected by a silicon photodiode located at a 90 angle from the laser A light...

Страница 18: ... 5 psi Nitrogen or other inert gas Gas Consumption 2 5 SLPM Operating Conditions Intended for indoor use only 15 6 to 26 7 C 60 to 85 F and 90 R H non condensing Electrical Requirements Nominal 120 VAC 50 60 Hz or Nominal 234 VAC 50 60 Hz 600 watts Fuse Size 120 VAC 240 VAC 6 25A 3 15A Wetted Materials Stainless steel glass anodized aluminum Teflon Light Source 670 nm Laser Diode 5 mW Detector Her...

Страница 19: ...ug the modular power cord provided into the power input module on the back of the detector The power cord is the dis connect device for the unit Therefore select a power outlet that is nearby and unobstructed allowing easy access for discon nection in the event of an emergency 3 2 2 Exhaust The ELSD exhaust is a O D stainless steel tube Use I D tubing to connect to a fume hood during operation Alt...

Страница 20: ...e GAS INLET port on the back of the unit WARNING Use only Nitrogen or Argon The internal gas regulator maintains the gas pressure at the factory set value displayed on the front panel 3 2 7 Fluid Connections Connect the outlet from your column to the LIQUID INLET on the front of the unit The length and volume between the column outlet and the detector inlet should be kept as short as possible to a...

Страница 21: ...meters for purifi cation runs and fraction collection To connect the ELSD to the Rf200 1 On the Rf200 disconnect the tubing assembly from ports C and D Retain the tubing assembly in case it is needed again later Figure 3 2 Preparing the Rf200 connection ports 2 Connect the shorter tubing assembly to port C and the lon ger tubing assembly to port D 3 Connect the other ends of the tubing to either s...

Страница 22: ...gure 3 4 Connecting gas supply to ELSD 5 Connect the external detector cable Isco part 250 0000 20 to the Rf200 Figure 3 5 6 Connect the other end of the cable to the ELSD taking care to observe polarity as indicated by the small tab on the ground side of the connector Figure 3 5 Rf external detector cable connections Ground CU Ground Rf200 Connection ELSD Connection ...

Страница 23: ...marked D according to the 4X instructions Figure 3 6 Component arrangement for connection with Rf200 and 4X module 3 Connect the 4X tubing marked D to the shorter tubing assembly from the 340C kit using the coupler as shown in Figure 3 7 Figure 3 7 Coupler for connecting 4X tubing 4 Connect the other end of the shorter tubing assembly to the tee fitting on the back of the ELSD detector 4X Module R...

Страница 24: ...e rear panel of either a Teledyne Isco Foxy R2 fraction collector or a Teledyne Isco frac tionation valve For complete information about connecting to the CombiFlash Torrent refer to Section 2 of the Torrent user manual 3 4 Conversion for HPLC Applications The following instructions are for preparation of the ELSD for HPLC applications Additional fittings connectors for HPLC pump connection are us...

Страница 25: ... and the Liquid Inlet plug from the front panel Figure 3 10 Removal of Liquid Inlet plug 4 Cut the tape to free the PTFE line from the drift tube 5 Disconnect the PTFE line from the internal pump Liquid Inlet plug hidden behind gusset Tape cut PTFE tubing PTFE tubing pump connection Pump motor 1 4 nuts 4 Drift tube Remove gusset Remove nut ...

Страница 26: ... 11 Disconnect PTFE line from pump 6 Route the line from the pump out through the Liquid Inlet opening in the front panel 7 Disconnect the harness from the pump motor Figure 3 12 Disconnect pump harness 8 On the Home Screen set the Gain GX to HPLC ...

Страница 27: ...ve minutes Once the unit is in the standby state turn off the laser and heaters If there are no leaks between your gas source and the ELSD you do not have to turn the gas off at the source Table 3 1 Suggested Operating Temperatures for HPLC Gradient Separations Solvent 1 0mL min Drift Tube Temperature C Spray Chamber Temperature C 90 10 Water METHANOL 45 15 90 10 Methanol WATER 65 50 50 50 Methano...

Страница 28: ...n Installer Initials Operator Initials 3 1 Unpacking 3 1 Unpacking Connections 3 2 1 Power 3 2 2 Exhaust 3 2 4 Communication 3 2 5 Digital I O Connections 3 2 6 Gas Connections 3 2 7 Fluid Connections 3 2 8 Nebulization Chamber Drain 3 3 1 Connecting to the CombiFlash Rf200 3 3 3 Connecting to the CombiFlash Torrent Module Certification of Section 3 Completion Installer Name print Installer Signat...

Страница 29: ...d off when the optics cell is cooling to a lower temperature 4 Laser power The green light is illuminated when the laser is on and stable and off when the laser is off The light blinks when laser power is low or unstable 5 Gas Supply The green light is on when the nebulizer gas pressure is within factory set limits and the gas valve is open The green light will blink if a pressure error exists The...

Страница 30: ...id is closed shutting off the flow of the nebuli zation gas the laser is turned off and the heaters are disabled To turn the power off press and hold the MENU POWER key for 5 seconds Wait at least 10 seconds before turning the instrument on after it has been turned off 4 1 4 Autozero Key When the AUTOZERO key is depressed the ELS Detection signal will reset to about 20mV An audible beep is heard w...

Страница 31: ...displayed If there is an asterisk at the upper right corner pushing MENU POWER key will bring up a screen to explain what s wrong XX SC DT OC ET RST BLN GAS FLT or BFT CL GX FS SC Spray Chamber setpoint 10 C to 70 C readout range 10 0 C to 120 0 C DT Drift Tube setpoint 22 C to 120 C readout range 10 0 C to120 0 C OC Optical Cell setpoint 22 C to 70 C readout range 10 0 C to 120 0 C ET Exhaust Tub...

Страница 32: ... reading Menu Screen Pressing the MENU POWER key once when no error conditions exist or twice times if an error condition exists enters the menu screen Push or keys to select the menus Push MENU POWER twice to return to the Home screen Available Menus Spray Chamber Page Sets Spray Chamber temper ature Drift Tube Page Sets Drift Tube optical cell and exhaust tube temperatures Control Page Sets Run ...

Страница 33: ...oint at 1 C increment Pushing MENU POWER key to accept the change and go back to Home screen Pushing AUTOZERO key to abolish the change and goes back to Home screen Control Page Run Standby Laser Off or Laser On Turn off Sound or Turn on Sound Use or keys to select an action Push MENU POWER key to take an action and go back Home screen Push AUTOZERO key to keep the current state and go back Home s...

Страница 34: ...to choose from FLT and BFT The filter type is selected at the top of the menu after weight 10 Select FLT or BFT and push the MENU POWER KEY to enter Then enter the filter menu again and selected the appropriate filter weight Filter weight is the level of baseline noise filtration OFF indi cates no filtration 10 is maximum filtration In most cases select BFT for baseline filtering The FLT setting a...

Страница 35: ...t conditions to the selected method number A method includes Spray Chamber Temperature Setpoint Drift Tube Temperature Setpoint Filter Weight Calibration Factor and Detector Gain Calibration Page 20 to 100 in 1 increments Use or keys to select an action Use 20 with Combi Flash systems Push MENU POWER key to take an action and go back Home screen Push AUTOZERO key to keep the current state and go b...

Страница 36: ...parameter Standby Countdown Screen Pressing the MENU POWER key twice when no error conditions exist or three times if an error condition exists enters the standby countdown screen Press MENU POWER and hold for 5 seconds if you wish to turn off the detector immediately It is rec ommended that the standby mode be activated when shutting down the detector The timer allows enough time for the vapor to...

Страница 37: ... and the system is pressurized the pump will automatically start to deliver the mobile phase to the detector Monitor the Thermo Split drain on the front of the unit It may take up to 10 minutes flowrate 0 75ml min to see liquid coming out of the drain tube if running reverse phase solvent If running normal phase solvents there may not be any flow from the drain tube 6 An asterisk may be blinking a...

Страница 38: ...apo rates shifting the particle size distribution low enough for essen tially all the particles to negotiate the bend So when highly organic mobile phases are used the Thermo Split chamber is used at ambient or elevated temperatures Under these condi tions a majority of the aerosol particles pass through the chamber and are carried into the evaporative zone For difficult to evaporate mobile phases...

Страница 39: ...e drift tube was held content at 70 C and the spray chamber was lowered The signal height decreased as the spray chamber temperature decreased because more of the vapor was diverted from the Drift Tube and sent to the drain Figure 4 2 Spray Chamber Temperature Effects Elevated Drift Tube Temperature The drift tube temperature can be set from ambient to 120 C The drift tube temperature is set at a ...

Страница 40: ...int The higher the flowrate of an aqueous mobile phase the lower the spray chamber temperature will need to be High flowrates of volatile mobile phases may require a higher drift tube temperature The ELSD may perform best at sub ambient spray chamber temperatures if the flowrate is extremely high even for volatile mobile phases Gradient Separations Suggested Operating Temperatures For gradient sep...

Страница 41: ... and comparing the noise to other known solvents We have found that not all HPLC grade solvents are acceptable for use with an ELSD Preservatives commonly used in Tetrahydro furan THF will increase the noise level If unstabilized THF is used ensure that it is fresh THF can contain peroxides that can increase noise and are potentially explosive if taken to dryness Mobile Phase Flowrate and Composit...

Страница 42: ...recommended that the chromatographic column be flushed with at least 10 column volumes before it is connected to the ELSD 4 5 Default Method The ELSD s default method Method 0 is factory set with run parameters that typically produce optimal sensitivity across a broad range of solvent types Spray Chamber SC 35 C Drift Tube DT 45 C Filter FLT 5 Calibration CL 20 Gain GX PREP Full Scale FS 5V ...

Страница 43: ...rol and Laboratory Use Standard Description Severity Applied Performance Criteria EN61000 4 2 Electrostatic Discharge Level 2 4kV contact discharge Level 3 8kV air discharge A EN61000 4 3 Radiated RF Immunity 80 MHz to 2 7Ghz 80 AM at 1kHz Level 2 3V m A EN61000 4 4 Electrical Fast Transient Level 2 1kV on AC lines A EN61000 4 5 Surge on AC Lines Level 2 1kV common mode Level 2 0 5KV differential ...

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Страница 45: ...e accident or lack of proper maintenance This warranty does not cover products not sold under the Teledyne Isco trademark or for which any other warranty is specifically stated No item may be returned for warranty service without a return authorization number issued by Teledyne Isco This warranty is expressly in lieu of all other warranties and obligations and Teledyne Isco specifically disclaims ...

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